Kinetic Transition in Amyloid Assembly as a Screening Assay for Oligomer-Selective Dyes
Abstract
:1. Introduction
2. Materials and Methods
2.1. Protein and Chemicals
2.2. Preparation of HewL, Aβ40, Aβ42, CV, and ThT Solutions
2.3. Measuring Amyloid Growth Kinetics with Thioflavin T and Crystal Violet
2.4. Atomic Force Microscopy
2.5. Transmission Electron Microscopy
2.6. Fourier-Transform Infrared Spectroscopy (FTIR)
3. Results
3.1. Crystal Violet as Oligomer-Selective Indicator Dye
3.2. CV and ThT Report Identical Amyloid Kinetics but with Distinctly Different Sensitivities
3.3. Separating Amyloid Assembly into its gO/CF and RF Components
3.4. Biphasic Kinetics of Alzheimer Peptides Aβ40 and Aβ42 upon Onset of gO/CF Formation
4. Discussion
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Barton, J.; Arias, D.S.; Niyangoda, C.; Borjas, G.; Le, N.; Mohamed, S.; Muschol, M. Kinetic Transition in Amyloid Assembly as a Screening Assay for Oligomer-Selective Dyes. Biomolecules 2019, 9, 539. https://doi.org/10.3390/biom9100539
Barton J, Arias DS, Niyangoda C, Borjas G, Le N, Mohamed S, Muschol M. Kinetic Transition in Amyloid Assembly as a Screening Assay for Oligomer-Selective Dyes. Biomolecules. 2019; 9(10):539. https://doi.org/10.3390/biom9100539
Chicago/Turabian StyleBarton, Jeremy, D. Sebastian Arias, Chamani Niyangoda, Gustavo Borjas, Nathan Le, Saefallah Mohamed, and Martin Muschol. 2019. "Kinetic Transition in Amyloid Assembly as a Screening Assay for Oligomer-Selective Dyes" Biomolecules 9, no. 10: 539. https://doi.org/10.3390/biom9100539