The Effect of Dimethyl Sulfoxide on the Lysozyme Unfolding Kinetics, Thermodynamics, and Mechanism
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. The Differential Scanning Calorimetry (DSC) Method
2.3. Circular Dichroism (CD) Spectroscopy Study of Unfolding Equilibrium
2.4. Spectrofluorimetric Study of Unfolding Equilibrium
2.5. Spectrofluorimetric Study of Unfolding Kinetics
3. Results
3.1. DSC Experiments
3.2. Circular Dichroism Spectroscopy
3.3. Spectrofluorimetry
3.4. Notes on Kinetic Models of Denaturation
3.5. Isothermal Kinetics of Lysozyme Unfolding
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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x1 | Td (K) | ΔdHcal (kJ·mol−1) |
---|---|---|
0 | 351.0 | 494 ± 10 |
0.05 | 347.6 | 539 ± 9 |
0.1 | 344.7 | 569 ± 10 |
0.2 | 337.2 | 508 ± 9 |
0.25 | 331.3 | 476 ± 9 |
0.3 | 326.0 | 415 ± 12 |
0.35 | 319.4 | 337 ± 18 |
x1 | v (K·min−1) | Td (K) | Td(eq). (K) |
---|---|---|---|
0 | 0.1 | 350.4 | 350.3 |
0.25 | 350.4 | ||
0.5 | 350.6 | ||
1.0 | 351.0 | ||
2.0 | 351.4 | ||
0.05 | 0.5 | 347.2 | 347.0 |
1.0 | 347.6 | ||
2.0 | 348.1 | ||
0.10 | 0.5 | 343.9 | 343.7 |
1.0 | 344.7 | ||
2.0 | 345.2 | ||
0.20 | 0.5 | 336.5 | 336.1 |
1.0 | 337.2 | ||
2.0 | 338.1 | ||
0.25 | 0.5 | 330.2 | 329.4 |
1.0 | 331.3 | ||
2.0 | 332.9 | ||
0.30 | 0.5 | 324.4 | 323.8 |
1.0 | 326.0 | ||
2.0 | 327.2 | ||
0.35 | 0.5 | 316.1 | 312.8 |
1.0 | 319.4 |
x1 | Td − TCD (K) |
---|---|
0 | 0.6 |
0.1 | 1.7 |
0.2 | 3.6 |
0.25 | 4.4 |
0.3 | 7.3 |
0.35 | 11.7 |
x1 | E1 (kJ·mol−1) | ln(A1/min−1) |
---|---|---|
0.25 | 231 ± 20 | 86.6 ± 4 |
0.30 | 233 ± 18 | 88.5 ± 4 |
0.35 | 222 ± 15 | 85.7 ± 2 |
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Magsumov, T.; Fatkhutdinova, A.; Mukhametzyanov, T.; Sedov, I. The Effect of Dimethyl Sulfoxide on the Lysozyme Unfolding Kinetics, Thermodynamics, and Mechanism. Biomolecules 2019, 9, 547. https://doi.org/10.3390/biom9100547
Magsumov T, Fatkhutdinova A, Mukhametzyanov T, Sedov I. The Effect of Dimethyl Sulfoxide on the Lysozyme Unfolding Kinetics, Thermodynamics, and Mechanism. Biomolecules. 2019; 9(10):547. https://doi.org/10.3390/biom9100547
Chicago/Turabian StyleMagsumov, Timur, Alisa Fatkhutdinova, Timur Mukhametzyanov, and Igor Sedov. 2019. "The Effect of Dimethyl Sulfoxide on the Lysozyme Unfolding Kinetics, Thermodynamics, and Mechanism" Biomolecules 9, no. 10: 547. https://doi.org/10.3390/biom9100547