Characterization of Terpene Synthase from Tea Green Leafhopper Being Involved in Formation of Geraniol in Tea (Camellia sinensis) Leaves and Potential Effect of Geraniol on Insect-Derived Endobacteria
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials and Treatments
2.2. Collection and Analysis of Geraniol
2.3. Extraction and Assay Activity of Geraniol Synthase from Tea Leaves
2.4. Extraction and Activity Assay of Geraniol Synthase from Empoasca (Matsumurasca) onukii
2.5. Empoasca (Matsumurasca) onukii Terpene Synthase Genes Cloning
2.6. Recombinant Expression of Empoasca (Matsumurasca) onukii Terpene Synthases in Escherichia Coli
2.7. Recombinant Expression of Empoasca (Matsumurasca) onukii Terpene Synthases in Insect Cell Sf9
2.8. Recombinant Empoasca (Matsumurasca) onukii Terpene Synthase Enzyme Assay
2.9. Extraction and Activity Assay of Geraniol Synthase from Other Insects
2.10. Empoasca (Matsumurasca) onukii Endobateria Isolation, Identification, and Antibacterial Activity Assay of Geraniol
2.11. Statistical Analyses
3. Results
3.1. Empoasca (Matsumurasca) onukii Attack Significantly Increased Geraniol Emission from Tea Leaves
3.2. Crude Enzyme Extract of Empoasca (Matsumurasca) onukii Produced Geraniol from Greanyl Diphosphate
3.3. Terpene synthase from Empoasca (Matsumurasca) onukii Was Able to Convert Greanyl Diphosphate to Geraniol
3.4. Geraniol Synthase Also Existed in Other Insects
3.5. Geraniol Had in Vitro Ability to Inhibit the Growth of Endobacterial Isolated from Empoasca (Matsumurasca) onukii
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Geraniol Concentration (μg/mL) | OD600 |
---|---|
0 | >3 |
100 | >3 |
250 | 11.980 ± 0.232 |
500 | 0.018 ± 0.006 |
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Zhou, Y.; Liu, X.; Yang, Z. Characterization of Terpene Synthase from Tea Green Leafhopper Being Involved in Formation of Geraniol in Tea (Camellia sinensis) Leaves and Potential Effect of Geraniol on Insect-Derived Endobacteria. Biomolecules 2019, 9, 808. https://doi.org/10.3390/biom9120808
Zhou Y, Liu X, Yang Z. Characterization of Terpene Synthase from Tea Green Leafhopper Being Involved in Formation of Geraniol in Tea (Camellia sinensis) Leaves and Potential Effect of Geraniol on Insect-Derived Endobacteria. Biomolecules. 2019; 9(12):808. https://doi.org/10.3390/biom9120808
Chicago/Turabian StyleZhou, Ying, Xiaoyu Liu, and Ziyin Yang. 2019. "Characterization of Terpene Synthase from Tea Green Leafhopper Being Involved in Formation of Geraniol in Tea (Camellia sinensis) Leaves and Potential Effect of Geraniol on Insect-Derived Endobacteria" Biomolecules 9, no. 12: 808. https://doi.org/10.3390/biom9120808