Improved Cordycepin Production by Cordyceps militaris KYL05 Using Casein Hydrolysate in Submerged Conditions
Abstract
:1. Introduction
2. Experimental
2.1. Microorganisms
2.2. Media and Culture Conditions
2.2.1. Seed Culture of C. Militaris
2.2.2. Main Culture of C. Militaris
2.2.3. Effects of Temperature, Initial pH, and Shaking Speed
2.2.4. Effects of Carbon Source and Nitrogen Source
2.3. Analytical Methods
3. Results and Discussion
3.1. Microbial Strain Selection for Cordycepin Production
3.2. Determination of Culture Conditions for Cordycepin Production
3.3. Effect of Carbon and Nitrogen Source on Cordycepin Production
4. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Strain | Culture Conditions | Carbon Source (g/L) | Nitrogen Source (g/L) | Cordycepin Production (mg/L) | Cordycepin Productivity (mg/L∙day) | Ref. | |||
---|---|---|---|---|---|---|---|---|---|
Temp. (°C) | RPM | pH | Time (day) | ||||||
C. militaris | 25 | 110 | 6 | 20 | Glucose 40 | Peptone 10 | 597 | 29.9 | [9] |
C. militaris | 25 | 110 | 4.8 | 18 | Glucose 42 | Peptone 16 | 245 | 13.6 | [30] |
C. militaris | 25 | 110 | 5 | 17 | Glucose 10 | Peptone 10 | 346 | 20.4 | [32] |
C. militaris KCTC6862 | 24 | 120 | 6.5 | 5 | Glucose 10 | Peptone 10 | 22 | 4.4 | [33] |
C. militaris KCTC16932 | 24 | 120 | 6.5 | 5 | Glucose 10 | Peptone 10 | 23 | 4.6 | |
C. militaris DGUM32003 | 24 | 120 | 6.5 | 5 | Glucose 10 | Peptone 10 | 39 | 7.8 | |
C. militaris | 25 | 200 | 4.8 | 17 | Glucose 40 | Peptone 10 | 201 | 11.8 | [34] |
C. militaris KYL05 | 25 | 150 | 6 | 6 | Glucose 20 | CH 20 | 445 | 74.2 | This study |
Amino Acid | Amino Acid Concentration (g-Amino Acid/100 g-Nitrogen Source) | ||
---|---|---|---|
CH | Yeast Extract | Peptone | |
Aspartic acid | 6.12 | 6.54 | 5.82 |
Threonine | 3.69 | 3.19 | 2.18 |
Serine | 4.42 | 3.29 | 3.33 |
Glutamate | 18.01 | 12.44 | 9.97 |
Proline | 7.48 | 2.80 | 10.13 |
Glycine | 1.69 | 3.07 | 20.00 |
Alanine | 2.75 | 5.75 | 8.87 |
Valine | 5.58 | 4.16 | 2.88 |
Isoleucine | 4.33 | 3.45 | 1.76 |
Leucine | 7.46 | 4.92 | 3.48 |
Tyrosine | 3.19 | 0.70 | 0.48 |
Phenylalanine | 3.88 | 2.90 | 2.15 |
Lysine | 6.65 | 5.29 | 3.89 |
Histidine | 2.11 | 1.38 | 0.82 |
Arginine | 2.84 | 3.41 | 7.10 |
Cysteine | 0.47 | 1.07 | 0.34 |
Methionine | 2.46 | 1.08 | 0.84 |
Total content | 83.13 | 65.42 | 84.06 |
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Lee, S.K.; Lee, J.H.; Kim, H.R.; Chun, Y.; Lee, J.H.; Yoo, H.Y.; Park, C.; Kim, S.W. Improved Cordycepin Production by Cordyceps militaris KYL05 Using Casein Hydrolysate in Submerged Conditions. Biomolecules 2019, 9, 461. https://doi.org/10.3390/biom9090461
Lee SK, Lee JH, Kim HR, Chun Y, Lee JH, Yoo HY, Park C, Kim SW. Improved Cordycepin Production by Cordyceps militaris KYL05 Using Casein Hydrolysate in Submerged Conditions. Biomolecules. 2019; 9(9):461. https://doi.org/10.3390/biom9090461
Chicago/Turabian StyleLee, Soo Kweon, Ju Hun Lee, Hyeong Ryeol Kim, Youngsang Chun, Ja Hyun Lee, Hah Young Yoo, Chulhwan Park, and Seung Wook Kim. 2019. "Improved Cordycepin Production by Cordyceps militaris KYL05 Using Casein Hydrolysate in Submerged Conditions" Biomolecules 9, no. 9: 461. https://doi.org/10.3390/biom9090461