Genome-Wide Identification and Characterization of WRKY Transcription Factors and Their Expression Profile in Loropetalum chinense var. rubrum
Abstract
:1. Introduction
2. Results
2.1. Identification and Characterization of LcWRKYs
2.2. Phylogenetic Analysis of the LcWRKYs, Classification, and Multiple Sequence Alignment
2.3. Analysis of Conserved Motifs and Gene Structures
2.4. cis-Element Analysis of LcWRKY Genes
2.5. Duplication and Synteny Analysis of LcWRKY Genes
2.6. Expression Analysis of the LcWRKY Genes in Leaves and Flowers of Different Cultivars
2.7. Expression Analysis of the LcWRKY Genes in Leaves at Different Developmental Stages
2.8. The Expression Analysis of the LcWRKY Genes under White and Blue Light Treatment
3. Discussion
4. Materials and Methods
4.1. Identification, Chromosome Localization, and Characteristic Analysis of LcWRKYs
4.2. Construction of Phylogenetic Trees and Multiple Sequence Alignment
4.3. Conserved Motifs and Gene Structure Analysis
4.4. cis-Regulatory Element Analysis
4.5. Gene Duplication, Calculation of (ka/ks) Non-Synonymous/Synonymous Ratio, and Synteny Analysis
4.6. Generation of Transcriptome Data and the Gene Expression Pattern Analysis
4.7. Plant Material and Treatments
4.8. Real-Time Fluorescence Quantitative PCR
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Liu, Y.; Zhang, Y.; Liu, Y.; Lin, L.; Xiong, X.; Zhang, D.; Li, S.; Yu, X.; Li, Y. Genome-Wide Identification and Characterization of WRKY Transcription Factors and Their Expression Profile in Loropetalum chinense var. rubrum. Plants 2023, 12, 2131. https://doi.org/10.3390/plants12112131
Liu Y, Zhang Y, Liu Y, Lin L, Xiong X, Zhang D, Li S, Yu X, Li Y. Genome-Wide Identification and Characterization of WRKY Transcription Factors and Their Expression Profile in Loropetalum chinense var. rubrum. Plants. 2023; 12(11):2131. https://doi.org/10.3390/plants12112131
Chicago/Turabian StyleLiu, Yang, Yifan Zhang, Yang Liu, Ling Lin, Xingyao Xiong, Donglin Zhang, Sha Li, Xiaoying Yu, and Yanlin Li. 2023. "Genome-Wide Identification and Characterization of WRKY Transcription Factors and Their Expression Profile in Loropetalum chinense var. rubrum" Plants 12, no. 11: 2131. https://doi.org/10.3390/plants12112131