Integrating Full-Length Transcriptome and RNA Sequencing of Siberian Wildrye (Elymus sibiricus) to Reveal Molecular Mechanisms in Response to Drought Stress
Abstract
:1. Introduction
2. Results
2.1. The General Features of the Third-Generation and Second-Generation RNA-Seq
2.2. Differentially Expressed Genes under Drought Stress
2.3. Gene Expression Patterns between Two Genotypes with Distinct Drought Tolerance
2.4. Responses of E. sibiricus under Short-Term and Long-Term Drought Stress
2.5. Gene Expression Pattern of E. sibiricus in Response to Drought Stress
2.6. Key Drought Stress Response Pathways and Genes Identified
2.7. WGCNA Identifies Drought Stress Response Transcription Factors and Genes
3. Materials and Methods
3.1. Plant Materials, Drought Treatments and Sample Collection
3.2. RNA Extraction and Sequencing
3.3. Analysis of Full-Length Transcriptome Data
3.4. Analysis of RNA-Seq
3.5. Quantitative Real-Time PCR (qRT-PCR) Analysis
4. Discussion
4.1. Phenotypic and Genetic Differences of E. sibiricus in Response to Drought Stress
4.2. Metabolic Pathways of E. sibiricus in Response to Drought Stress
4.3. Drought-Responsive Transcription Factors of E. sibiricus
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Yu, Q.; Xiong, Y.; Su, X.; Xiong, Y.; Dong, Z.; Zhao, J.; Shu, X.; Bai, S.; Lei, X.; Yan, L.; et al. Integrating Full-Length Transcriptome and RNA Sequencing of Siberian Wildrye (Elymus sibiricus) to Reveal Molecular Mechanisms in Response to Drought Stress. Plants 2023, 12, 2719. https://doi.org/10.3390/plants12142719
Yu Q, Xiong Y, Su X, Xiong Y, Dong Z, Zhao J, Shu X, Bai S, Lei X, Yan L, et al. Integrating Full-Length Transcriptome and RNA Sequencing of Siberian Wildrye (Elymus sibiricus) to Reveal Molecular Mechanisms in Response to Drought Stress. Plants. 2023; 12(14):2719. https://doi.org/10.3390/plants12142719
Chicago/Turabian StyleYu, Qingqing, Yi Xiong, Xiaoli Su, Yanli Xiong, Zhixiao Dong, Junming Zhao, Xin Shu, Shiqie Bai, Xiong Lei, Lijun Yan, and et al. 2023. "Integrating Full-Length Transcriptome and RNA Sequencing of Siberian Wildrye (Elymus sibiricus) to Reveal Molecular Mechanisms in Response to Drought Stress" Plants 12, no. 14: 2719. https://doi.org/10.3390/plants12142719