RNA-Seq-Based WGCNA and Association Analysis Reveal the Key Regulatory Module and Genes Responding to Salt Stress in Wheat Roots
Abstract
:1. Introduction
2. Results
2.1. RNA-Seq Data Evaluation
2.2. Gene Co-Expression Modules Responding to Salt Stress in Roots
2.3. The GO Enrichment of the Blue Module
2.4. Association Analysis of the DEGs from the Key Terms
2.5. Candidate Genes Responding to Salt Stress in Roots
2.6. Gene Co-Expression Networks Responding to Short-Term Salt Stress in Roots
3. Discussion
3.1. The Blue Module Is Related to the Time Course of Salt-Stress Response in Wheat Roots
3.2. Four Novel Candidate Genes Responding to Salt Stress Discovered from the Blue Module
3.3. A Predicted Gene Co-Expression Network for Salt Tolerance
4. Materials and Methods
4.1. Plant Materials and Salt Treatment
4.2. RNA-Seq
4.3. Co-Expression Network Construction
4.4. Association Analysis
4.5. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Chen, J.; Zhang, L.; Liu, Y.; Shen, X.; Guo, Y.; Ma, X.; Zhang, X.; Li, X.; Cheng, T.; Wen, H.; et al. RNA-Seq-Based WGCNA and Association Analysis Reveal the Key Regulatory Module and Genes Responding to Salt Stress in Wheat Roots. Plants 2024, 13, 274. https://doi.org/10.3390/plants13020274
Chen J, Zhang L, Liu Y, Shen X, Guo Y, Ma X, Zhang X, Li X, Cheng T, Wen H, et al. RNA-Seq-Based WGCNA and Association Analysis Reveal the Key Regulatory Module and Genes Responding to Salt Stress in Wheat Roots. Plants. 2024; 13(2):274. https://doi.org/10.3390/plants13020274
Chicago/Turabian StyleChen, Jiating, Lei Zhang, Yingxi Liu, Xinyao Shen, Yujing Guo, Xiaofei Ma, Xiaojun Zhang, Xin Li, Tianling Cheng, Huiqin Wen, and et al. 2024. "RNA-Seq-Based WGCNA and Association Analysis Reveal the Key Regulatory Module and Genes Responding to Salt Stress in Wheat Roots" Plants 13, no. 2: 274. https://doi.org/10.3390/plants13020274