Dowling, P.; et al. Characterization of Contractile Proteins from Skeletal Muscle Using Gel-Based Top-Down Proteomics. Proteomes 2019, 7, 25

Paul Dowling; Margit Zweyer; Dieter Swandulla; Kay Ohlendieck

doi:10.3390/proteomes7030028

,

and

¹

Department of Biology, Maynooth University, National University of Ireland, Maynooth, W23F2H6 Co. Kildare, Ireland

²

MU Human Health Research Institute, Maynooth University, Maynooth, W23F2H6 Co. Kildare, Ireland

³

Institute of Physiology II, University of Bonn, D-53115 Bonn, Germany

^*

Author to whom correspondence should be addressed.

Proteomes2019, 7(3), 28;https://doi.org/10.3390/proteomes7030028

This article belongs to the Special Issue Top-down Proteomics: In Memory of Dr. Alfred Yergey

Version Notes

Order Reprints

The authors wish to make the following correction to their paper [1]:

The Figure 2 and Figure 5 have some irregular typesetting. The corrected Figure 2 and Figure 5 should be:

Figure 2. Overview of the bioanalytical advantages versus potential technical limitations of gel-based top-down proteomics, especially in relation to two-dimensional gel electrophoresis (2DGE) approaches.

Figure 5. Overview of fluorescence two-dimensional difference in-gel electrophoresis (2D-DIGE) techniques for the comparative analysis of proteoforms. The upper panel shows the two main DIGE approaches for the systematic pre-electrophoretic labelling of protein fractions using fluorescent CyDyes. These methods utilise minimal sub-stochiometric labelling of assessable lysines in proteins or saturation labelling of assessable cysteines in proteins. The lower panel outlines three-dye (Cy2, Cy3, Cy5) versus two-dye (Cy3, Cy5) DIGE labelling methodology.

Reference

Dowling, P.; Zweyer, M.; Swandulla, D.; Ohlendieck, K. Characterization of Contractile Proteins from Skeletal Muscle Using Gel-Based Top-Down Proteomics. Proteomes 2019, 7, 25. [Google Scholar] [CrossRef] [PubMed]

© 2019 by the author. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

Dowling, P.; et al. Characterization of Contractile Proteins from Skeletal Muscle Using Gel-Based Top-Down Proteomics. Proteomes 2019, 7, 25

Reference

Article Metrics

Citations

Article Access Statistics