Immunomodulation of Oxidative Stress during Organ Donation Process: Preliminary Results
, Ana Rodríguez-Rodríguez 2, Luis Martín-Villén 1, María Mendoza-Prieto 1, Zaida Ruiz de Azúa-López 1, Lluis Sempere-Bordes 2, Laura Boyero-Corral 2, Domingo Daga-Ruiz 3, Antonio Gordillo-Brenes 4, María Pacheco-Sánchez 5, José Miguel Perez-Villares 6, Ángel Vilches-Arenas 7,* and Juan José Egea-Guerrero 1Round 1
Reviewer 1 Report
The manuscript titled “Melatonin Treatment for The Immunomodulation of Oxidative Stress in Transplant Organs during Controlled Donation after Circulatory Death. Preliminary Results from A Randomized Clinical Trial” is an interesting work. However, there are some significant drawbacks in the manuscript.
These are few comments that need to be addressed:
- The title is long and confusing. Rewording will be needed
- Abstract, lines 18-20, the objective of the work is not clear. Paraphrasing is required.
- Different font types in the abstract text should be avoided.
- Line 37, the word “organic” is not relevant
- Again, the last sentence (lines 73-75) of the introduction is not clear.
- Recipients’ and donors’ biological characteristics were not considered in analyzing the results or explaining the results in the discussion
- Mechanistic explanation of melatonin and a summary figure will be required
- The conclusion is only applicable to DCD but not DBD. Then also DCD is significant only at 0-90 but not 0-60. The conclusions are overstretched. The discussion and conclusion sections need to be updated/rewritten based on the results.
- Table 4 is missing from the text (though it’s there in the attached supplementary).
- Conclusions and experimental designs are not consistent. Also, factors associated with the effects of melatonin are not considered and discussed in the manuscript.
Author Response
Please see the attachment
Author Response File: 
Author Response.pdf
Reviewer 2 Report
Thank you for permitting me to review this manuscript
please better explain the difference between DBD and DCD
Line 51-54 please enumerate more molecules free radicals present in this process
Line 70 please provide a reference (PPR)
Is MDA measurement a significant part of oxyydative stress ? please develop
Line 87 Please define SNG
Fig 2 and table 3 the difference is not significant ?
since the difference is not significant , why the autors claim a reduction in oxydative stress ?
The authors should state assumptions and conclsions strictly based on their findings putative statements aare not justified when they are not supported by the results
Author Response
Please see the attachment
Author Response File: Author Response.pdf
Round 2
Reviewer 1 Report
Authors have addressed the comments to a reasonable extent.
Author Response
We want to thank this reviewer for his/her positive and constructive comments on our manuscript, we believe our paper has increased its quality following his/her suggestions.Reviewer 2 Report
The authors have improved the manuscript
Fig 4 is useless since table 4 provides the same result and can be deleted
the authors have provided only one result significantly different for MDA
A more cautious conclusion is needed
Author Response
Please see the attachment
Author Response File: Author Response.pdf
