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Biomedicines
Volume 10
Issue 12
10.3390/biomedicines10123100
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Article
Peer-Review Record

Immunohistochemical Detection of Estrogen Receptor-Beta (ERβ) with PPZ0506 Antibody in Murine Tissue: From Pitfalls to Optimization

Biomedicines 2022, 10(12), 3100; https://doi.org/10.3390/biomedicines10123100
by Sarah K. Schröder 1, Carmen G. Tag 1, Jan C. Kessel 1, Per Antonson 2 and Ralf Weiskirchen 1,*
Reviewer 2: Anonymous
Biomedicines 2022, 10(12), 3100; https://doi.org/10.3390/biomedicines10123100
Submission received: 7 November 2022 / Revised: 25 November 2022 / Accepted: 27 November 2022 / Published: 1 December 2022
(This article belongs to the Special Issue Editorial Board Members’ Collection Series: Utilization of Immune Checkpoint Inhibitors: Past, Present and Future Therapeutics in Cancer)

Round 1

Reviewer 1 Report

The article is very interesting. The research methodology and results were meticulously presented by the Authors. Thanks to this, the article has great potential for the instructions provided to be used in future research by other scientists.

A month ago, an article (doi: 10.1267/ahc.22-00043) on similar issues was published. Can the methodology and results of this study also be included in your discussion?

Author Response

Dear reviewer 1,

many thanks for reviewing our paper. Please find attached a pdf-File in which we describe how we dealt with your concerns/suggestions.

Regards

Ralf Weiskirchen

Author Response File: Author Response.pdf

Reviewer 2 Report

In this manuscript the authors present an immunohistochemical staining protocol to detect ERβ in mouse tissue. It is a well written manuscript with clearly presented methodology and results. I have the following comments:

1.       In which diseases has the role of ERβ been established?

2.       ERβ is expressed in the granulosa cells of the ovary. What is the role of hormonal treatment in granulosa cell tumours of the ovary? A short comment should be added to the introduction section.

3.       In the discussion section the authors should further discuss possible clinical implications of these findings. In what way do these findings have a potential to improve clinical practice?

Author Response

Dear reviewer 2,

many thanks for reviewing our paper. Please find attached a pdf-File in which we describe how we dealt with your concerns/suggestions.

Regards

Ralf Weiskirchen

Author Response File: Author Response.pdf

Reviewer 3 Report

The authors have done a wonderful job describing this novel technique of immunohistochemical detection of ER. I do not find a part of the study that required major modification; hence, i believe it can published in its present form.

Author Response

Dear reviewer 3,

many thanks for reviewing our paper. Please find attached a pdf-File in which we describe how we dealt with your concerns/suggestions.

Regards

Ralf Weiskirchen

Author Response File: Author Response.pdf

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