MicroRNA-138-5p Targets Pro-Apoptotic Factors and Favors Neural Cell Survival: Analysis in the Injured Spinal Cord
Abstract
:1. Introduction
2. Materials and Methods
2.1. Surgical Procedures
2.2. Histology, Fluorescent in Situ Hybridization (FISH), Immunofluorescence, and Image Analysis
2.3. Cell Culture
2.4. Computational Prediction of miR-138-5p Targets
2.5. Microarray Data
2.6. RT-qPCR Analysis
2.7. Cell Death Assays
2.7.1. Calcein/Propidium Iodide Assay
2.7.2. Terminal Deoxynucleotidyl Transferase-Mediated Deoxyuridine Triphosphate (dUTP) Nick End-Labeling (TUNEL) Assay
2.7.3. MTT Assay
2.7.4. Quantification of Cell Death Using Flow Cytometry
2.8. Dual Luciferase Reporter Gene Construction and 3′UTR Luciferase Reporter Assays
2.9. Immunoblot Assay
2.10. Measurement of Caspase-3/7 Activity
2.11. Data Analysis
3. Results
3.1. MiR-138-5p Becomes Downregulated among Spinal Neurons during Subacute SCI
3.2. MiR-138-5p Protects Neurons In Vitro
3.3. Predicting Novel Targets of miR-138-5p among the Genes Dysregulated after SCI
3.4. Reporter Assays Validate miR-138-5p Targeting on Casp3, Casp7, and Bak1 3′UTRs
3.5. MiR-138-5p Reduces the Gene and Protein Expression of Caspase 3, Caspase 7, and BAK
3.6. MiR-138-5p Attenuates Caspase-Dependent Apoptosis
3.7. Expression of Caspases 3 and 7 Increases after Subacute SCI and Negatively Correlates with the Downregulation of miR-18-5p
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Maza, R.M.; Barreda-Manso, M.A.; Reigada, D.; Silván, Á.; Muñoz-Galdeano, T.; Soto, A.; del Águila, Á.; Nieto-Díaz, M. MicroRNA-138-5p Targets Pro-Apoptotic Factors and Favors Neural Cell Survival: Analysis in the Injured Spinal Cord. Biomedicines 2022, 10, 1559. https://doi.org/10.3390/biomedicines10071559
Maza RM, Barreda-Manso MA, Reigada D, Silván Á, Muñoz-Galdeano T, Soto A, del Águila Á, Nieto-Díaz M. MicroRNA-138-5p Targets Pro-Apoptotic Factors and Favors Neural Cell Survival: Analysis in the Injured Spinal Cord. Biomedicines. 2022; 10(7):1559. https://doi.org/10.3390/biomedicines10071559
Chicago/Turabian StyleMaza, Rodrigo M., María Asunción Barreda-Manso, David Reigada, Ágata Silván, Teresa Muñoz-Galdeano, Altea Soto, Ángela del Águila, and Manuel Nieto-Díaz. 2022. "MicroRNA-138-5p Targets Pro-Apoptotic Factors and Favors Neural Cell Survival: Analysis in the Injured Spinal Cord" Biomedicines 10, no. 7: 1559. https://doi.org/10.3390/biomedicines10071559