Carfilzomib Mitigates Lipopolysaccharide/D-Galactosamine/Dimethylsulfoxide-Induced Acute Liver Failure in Mice
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Experimental Design
2.3. Serum Separation and Liver Preparations
2.4. Biochemical Analysis
2.5. Gene Expression
2.6. Western Blot
2.7. Histopathology
2.8. Statistical Analysis
3. Results
3.1. CFZ Decreases the LPS/GalN/DMSO-Induced Increase in ALT and AST in Serum
3.2. CFZ Decreases the LPS/GalN/DMSO-Induced Increase in Serum TNF-α, Hepatic NF-кB and Hepatic Caspase 3 Activity
3.3. CFZ Decreases the LPS/GalN/DMSO-Induced Increase in Hepatic COX-II
3.4. CFZ Ameliorates the LPS/GalN/DMSO-Induced Neutrophil Recruitment
3.5. CFZ Ameliorates the LPS/GalN/DMSO-Induced Altered Nitrosative and Oxidative Stress
3.6. CFZ Decreases LPS/GalN/DMSO-Caused-Histopathological Changes
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene | GenBank Accession # | Annealing Temperature | Orientation | Sequences (5’ to 3’) |
---|---|---|---|---|
COX-II | NM_011198 | 60 °C | Forward Reverse | TGGTGCCTGGTCTGATGATG GTGGTAACCGCTCAGGTGTTG |
iNOS | NM_010927 | 60 °C | Forward Reverse | CCTGGTACGGGCATTGCT GCTCATGCGGCCTCCTTT |
GAPDH | NM_008084 | 60 °C | Forward Reverse | TGAAGCAGGCATCTGAGGG CGAAGGTGGAAGAGTGGGAG |
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Alhareth, D.Y.; Alanazi, A.; Alanazi, W.A.; Ansari, M.A.; Nagi, M.N.; Ahmad, S.F.; Attia, M.S.M.; Nadeem, A.; Bakheet, S.A.; Attia, S.M. Carfilzomib Mitigates Lipopolysaccharide/D-Galactosamine/Dimethylsulfoxide-Induced Acute Liver Failure in Mice. Biomedicines 2023, 11, 3098. https://doi.org/10.3390/biomedicines11113098
Alhareth DY, Alanazi A, Alanazi WA, Ansari MA, Nagi MN, Ahmad SF, Attia MSM, Nadeem A, Bakheet SA, Attia SM. Carfilzomib Mitigates Lipopolysaccharide/D-Galactosamine/Dimethylsulfoxide-Induced Acute Liver Failure in Mice. Biomedicines. 2023; 11(11):3098. https://doi.org/10.3390/biomedicines11113098
Chicago/Turabian StyleAlhareth, Dhafer Y., Abdulrazaq Alanazi, Wael A. Alanazi, Mushtaq A. Ansari, Mahmoud N. Nagi, Sheikh F. Ahmad, Mohamed S. M. Attia, Ahmed Nadeem, Saleh A. Bakheet, and Sabry M. Attia. 2023. "Carfilzomib Mitigates Lipopolysaccharide/D-Galactosamine/Dimethylsulfoxide-Induced Acute Liver Failure in Mice" Biomedicines 11, no. 11: 3098. https://doi.org/10.3390/biomedicines11113098