Selective Inhibition of Cardiac Late Na+ Current Is Based on Fast Offset Kinetics of the Inhibitor
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Isolation of Cardiomyocytes
2.3. Electrophysiology
2.3.1. Action Potential Voltage Clamp
2.3.2. Conventional Voltage Clamp
2.3.3. Recording of APs from Multicellular Ventricular Preparations
2.3.4. Determination of Offset Kinetics
2.3.5. Determination of Onset Kinetics
2.4. Statistics
3. Results
3.1. Effects of GS967, Mexiletine and Quinidine under APVC Conditions
3.2. Effects of GS967, Mexiletine and Quinidine on INaL under Conventional Voltage Clamp Conditions
3.3. Effects of GS967, Mexiletine and Quinidine on Action Potential Upstroke
3.4. Selectivity of INaL over INaP Inhibition
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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Naveed, M.; Mohammed, A.S.A.; Topal, L.; Kovács, Z.M.; Dienes, C.; Ovári, J.; Szentandrássy, N.; Magyar, J.; Bányász, T.; Prorok, J.; et al. Selective Inhibition of Cardiac Late Na+ Current Is Based on Fast Offset Kinetics of the Inhibitor. Biomedicines 2023, 11, 2383. https://doi.org/10.3390/biomedicines11092383
Naveed M, Mohammed ASA, Topal L, Kovács ZM, Dienes C, Ovári J, Szentandrássy N, Magyar J, Bányász T, Prorok J, et al. Selective Inhibition of Cardiac Late Na+ Current Is Based on Fast Offset Kinetics of the Inhibitor. Biomedicines. 2023; 11(9):2383. https://doi.org/10.3390/biomedicines11092383
Chicago/Turabian StyleNaveed, Muhammad, Aiman Saleh A. Mohammed, Leila Topal, Zsigmond Máté Kovács, Csaba Dienes, József Ovári, Norbert Szentandrássy, János Magyar, Tamás Bányász, János Prorok, and et al. 2023. "Selective Inhibition of Cardiac Late Na+ Current Is Based on Fast Offset Kinetics of the Inhibitor" Biomedicines 11, no. 9: 2383. https://doi.org/10.3390/biomedicines11092383