Association of Telomere Length in T Lymphocytes, B Lymphocytes, NK Cells and Monocytes with Different Forms of Age-Related Macular Degeneration
Abstract
:1. Introduction
2. Materials and Methods
2.1. Demographic Characteristics of Study Participants
2.2. Isolation of Leukocytes and Monocytes from Peripheral Blood
2.3. Analysis of Leukocyte Telomere Length by Quantitative PCR (qPCR)
2.4. Statistics
3. Results
3.1. Cell Purity, DNA Integrity and Construction of Standard Curves
3.2. TLs in Different Subpopulations of Leukocytes from Control Patients
3.3. TLs in Leukocytes from Patients with Different Types of AMD
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Leukocytes | Control (n = 22) | NVAMD MA (n = 21) | NVAMD No MA (n = 20) | GA (n = 21) |
---|---|---|---|---|
T cells | 3.83 (2.28–5.62) | 8.9 (6.94–9.98) | 8.13 (6.94–9.26) | 5.7 (2.9–7.44) |
B cells | 8.34 (7.39–10.39) | 4.27 (2.82–5.85) | 5.49 (3.83–6.92) | 5.1 (3.54–6.75) |
NK cells | 5.83 (4.52–6.83) | 6.08 (3.69–7.86) | 7.07 (5.32–8.66) | 6.44 (5.11–8.36) |
Monocytes | 9.41 (8.41–10.42) | 2.77 (2.31–4.78) | 3.62 (2.89–5.46) | 5.13 (3.79–6.89) |
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Khalatyan, A.S.; Shishparenok, A.N.; Avetisov, K.S.; Gladilina, Y.A.; Blinova, V.G.; Zhdanov, D.D. Association of Telomere Length in T Lymphocytes, B Lymphocytes, NK Cells and Monocytes with Different Forms of Age-Related Macular Degeneration. Biomedicines 2024, 12, 1893. https://doi.org/10.3390/biomedicines12081893
Khalatyan AS, Shishparenok AN, Avetisov KS, Gladilina YA, Blinova VG, Zhdanov DD. Association of Telomere Length in T Lymphocytes, B Lymphocytes, NK Cells and Monocytes with Different Forms of Age-Related Macular Degeneration. Biomedicines. 2024; 12(8):1893. https://doi.org/10.3390/biomedicines12081893
Chicago/Turabian StyleKhalatyan, Anait S., Anastasiya N. Shishparenok, Konstantin S. Avetisov, Yulia A. Gladilina, Varvara G. Blinova, and Dmitry D. Zhdanov. 2024. "Association of Telomere Length in T Lymphocytes, B Lymphocytes, NK Cells and Monocytes with Different Forms of Age-Related Macular Degeneration" Biomedicines 12, no. 8: 1893. https://doi.org/10.3390/biomedicines12081893