Prior Appendicectomy and Gut Microbiota Re-Establishment in Adults after Bowel Preparation and Colonoscopy
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Design
2.2. Study Participants and Setting
2.3. Data Collection
2.4. Intervention
2.5. Outcome Measures
2.6. Covariates
2.7. DNA Extraction
2.8. Sequencing and Annotation
2.9. Pre-Processing
2.10. Statistical Analyses
3. Results
3.1. Participant Characteristics
3.2. Between-Group Differences in Gut Microbiota Composition at Baseline and Follow Up
3.3. Within-Group Changes in Gut Microbiota Composition Post-Procedure
3.4. Post Hoc Analyses of Appendicectomy as an Effect Modifier of Genus Re-Establishment
4. Discussion
4.1. Summary of Findings
4.2. Potential Clinical Implications
4.3. Challenges to Studying the Potential Function of the Human Appendix
4.4. Limitations of the Present Study
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Variable | With Appendix | Without Appendix |
---|---|---|
Participants, n (%) | 45 (78%) | 13 (22%) |
Age, mean (SD) | 58.4 (11.1) | 60.5 (9.9) |
Sex, female n (%) | 26 (58%) | 5 (39%) |
BMI, mean (SD) | 30.9 (6.5) | 26.0 (4.8) |
Currently smoking, yes n (%) | 5 (11%) | 1 (8%) |
IBS, yes n (%) | 11 (24%) | 2 (15%) |
Missing | 1 (2.2%) | 0 (0%) |
Diet quality ‡, mean (SD) | 44.2 (11.5) | 48.9 (11.4) |
Depression symptomatology, mean (SD) | 4.13 (3.31) | 2.15 (1.21) |
Depression severity, mean (SD) | 4.29 (5.88) | 1.15 (2.15) |
Anxiety symptomatology, mean (SD) | 5.78 (3.98) | 3.92 (2.56) |
Physical quality of life, mean (SD) | 0.689 (0.178) | 0.790 (0.103) |
Psychosocial quality of life, mean (SD) | 0.456 (0.213) | 0.581 (0.173) |
Overall quality of life, mean (SD) | 0.746 (0.199) | 0.868 (0.106) |
Gastrointestinal medications, yes n (%) | ||
Hyperacidity/reflux medication, | 19 (42%) | 3 (23%) |
Digestive supplements/cholelitholytics | 1 (2%) | 1 (8%) |
Self-reported prior gastrointestinal disease, yes n (%) | ||
Hiatus hernia/oesophageal reflux | 5 (11%) | 1 (8%) |
Chronic diarrhoea | 3 (7%) | 0 (0%) |
Chronic gastritis | 1 (2%) | 0 (0%) |
Bowel cancer § | 1 (2%) | 0 (0%) |
Peptic ulcer disease § | 0 (0%) | 1 (8%) |
History of sleeve gastrectomy | 1 (2%) | 0 (0%) |
Missing | 1 (2%) | 1 (8%) |
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McGuinness, A.J.; O’Hely, M.; Stupart, D.; Watters, D.; Dawson, S.L.; Hair, C.; Berk, M.; Mohebbi, M.; Loughman, A.; Guest, G.; et al. Prior Appendicectomy and Gut Microbiota Re-Establishment in Adults after Bowel Preparation and Colonoscopy. Biomedicines 2024, 12, 1938. https://doi.org/10.3390/biomedicines12091938
McGuinness AJ, O’Hely M, Stupart D, Watters D, Dawson SL, Hair C, Berk M, Mohebbi M, Loughman A, Guest G, et al. Prior Appendicectomy and Gut Microbiota Re-Establishment in Adults after Bowel Preparation and Colonoscopy. Biomedicines. 2024; 12(9):1938. https://doi.org/10.3390/biomedicines12091938
Chicago/Turabian StyleMcGuinness, Amelia J., Martin O’Hely, Douglas Stupart, David Watters, Samantha L. Dawson, Christopher Hair, Michael Berk, Mohammadreza Mohebbi, Amy Loughman, Glenn Guest, and et al. 2024. "Prior Appendicectomy and Gut Microbiota Re-Establishment in Adults after Bowel Preparation and Colonoscopy" Biomedicines 12, no. 9: 1938. https://doi.org/10.3390/biomedicines12091938