Anti-Allergic and Anti-Inflammatory Effects of Lidocaine-Derived Organic Compounds in a House Dust Mite-Induced Allergic Rhinitis Mouse Model
Abstract
:1. Introduction
2. Materials and Methods
2.1. Reagents
2.2. Preparation of Lidocaine-Derived Organic Compounds
2.3. Development of DP-Induced Allergic Rhinitis Mouse Model and Experimental Protocol
2.4. Evaluation of Allergic Symptoms
2.5. Evaluation of Inflammatory Cells in Nasal Lavage Fluid (NLF)
2.6. Measurement of Serum Total IgE and Cytokines
2.7. Measurement of Cytokines and Transcription Factor mRNA in Sinonasal Mucosa
2.8. Measurement of Cytokines from Splenocytes Stimulated with DP
2.9. Histological Evaluation of Sinonasal Mucosa
2.10. Statistical Analysis
3. Results
3.1. Effects of Lidocaine Analogs on the Allergy Symptoms
3.2. Effect of Lidocaine Analogs on Inflammatory Cell Differentiation in NLF
3.3. Effect of Lidocaine Analogs on Serum Total IgE and Cytokine Levels
3.4. Effect of Lidocaine Analogs on Cytokines and the Expression of Their Transcription Factor mRNA in Sinonasal Mucosa
3.5. Effect of Lidocaine Analogs on DP-Induced Splenocyte Activation
3.6. Effect of Lidocaine Analogs on Histologic Changes in Sinonasal Mucosa
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Shin, S.-H.; Ye, M.-K.; Chae, M.-H.; Geum, S.-Y.; Aboraia, A.S.; Abdel-Aal, A.-B.M.; Qayed, W.S.; El-wahab, H.A.A.A.; Abou-Ghadir, O.F.; Aboul-Fadl, T. Anti-Allergic and Anti-Inflammatory Effects of Lidocaine-Derived Organic Compounds in a House Dust Mite-Induced Allergic Rhinitis Mouse Model. Biomedicines 2024, 12, 1965. https://doi.org/10.3390/biomedicines12091965
Shin S-H, Ye M-K, Chae M-H, Geum S-Y, Aboraia AS, Abdel-Aal A-BM, Qayed WS, El-wahab HAAA, Abou-Ghadir OF, Aboul-Fadl T. Anti-Allergic and Anti-Inflammatory Effects of Lidocaine-Derived Organic Compounds in a House Dust Mite-Induced Allergic Rhinitis Mouse Model. Biomedicines. 2024; 12(9):1965. https://doi.org/10.3390/biomedicines12091965
Chicago/Turabian StyleShin, Seung-Heon, Mi-Kyung Ye, Mi-Hyun Chae, Sang-Yen Geum, Ahmed S. Aboraia, Abu-Baker M. Abdel-Aal, Wesam S. Qayed, Hend A. A. Abd El-wahab, Ola F. Abou-Ghadir, and Tarek Aboul-Fadl. 2024. "Anti-Allergic and Anti-Inflammatory Effects of Lidocaine-Derived Organic Compounds in a House Dust Mite-Induced Allergic Rhinitis Mouse Model" Biomedicines 12, no. 9: 1965. https://doi.org/10.3390/biomedicines12091965