Biofabrication of Gingival Fibroblast Cell-Laden Collagen/Strontium-Doped Calcium Silicate 3D-Printed Bi-Layered Scaffold for Osteoporotic Periodontal Regeneration
Abstract
:1. Introduction
2. Materials and Methods
2.1. Fabrication of SrCS Scaffolds
2.2. Characterization of Physicochemical Properties of SrCS Scaffolds
2.3. Assessment of In Vitro Bioactivity
2.4. Fabrication of Cell-Laden Col Hydrogel and Bi-Layer Scaffold
2.5. Cellular Viability and Proliferation
2.6. Secretion Protein Analysis
2.7. Osteogenesis Capabilities
2.8. Establishment of Osteoporotic Animal Models
2.9. µCT Evaluation
2.10. Histology Evaluation
2.11. Statistical Analyses
3. Results and Discussion
3.1. The Characterizations of SrCS Scaffolds
3.2. In Vitro Bioactivity
3.3. Cytotoxicity and Proliferation of hGF Laden in Col Ink
3.4. Quantification of FGF-2, BMP-2, and VEGF Secreted from hGF Encapsulated in One-Layer or Bi-Layer Scaffold
3.5. Effect of hGF-Laden Col Bio-Ink on Osteogenesis
3.6. In Vivo Bone Regeneration
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Wang, C.-Y.; Chiu, Y.-C.; Lee, A.K.-X.; Lin, Y.-A.; Lin, P.-Y.; Shie, M.-Y. Biofabrication of Gingival Fibroblast Cell-Laden Collagen/Strontium-Doped Calcium Silicate 3D-Printed Bi-Layered Scaffold for Osteoporotic Periodontal Regeneration. Biomedicines 2021, 9, 431. https://doi.org/10.3390/biomedicines9040431
Wang C-Y, Chiu Y-C, Lee AK-X, Lin Y-A, Lin P-Y, Shie M-Y. Biofabrication of Gingival Fibroblast Cell-Laden Collagen/Strontium-Doped Calcium Silicate 3D-Printed Bi-Layered Scaffold for Osteoporotic Periodontal Regeneration. Biomedicines. 2021; 9(4):431. https://doi.org/10.3390/biomedicines9040431
Chicago/Turabian StyleWang, Chen-Ying, Yung-Cheng Chiu, Alvin Kai-Xing Lee, Yun-An Lin, Ping-Yi Lin, and Ming-You Shie. 2021. "Biofabrication of Gingival Fibroblast Cell-Laden Collagen/Strontium-Doped Calcium Silicate 3D-Printed Bi-Layered Scaffold for Osteoporotic Periodontal Regeneration" Biomedicines 9, no. 4: 431. https://doi.org/10.3390/biomedicines9040431
APA StyleWang, C. -Y., Chiu, Y. -C., Lee, A. K. -X., Lin, Y. -A., Lin, P. -Y., & Shie, M. -Y. (2021). Biofabrication of Gingival Fibroblast Cell-Laden Collagen/Strontium-Doped Calcium Silicate 3D-Printed Bi-Layered Scaffold for Osteoporotic Periodontal Regeneration. Biomedicines, 9(4), 431. https://doi.org/10.3390/biomedicines9040431