Regulation of MRP4 Expression by circHIPK3 via Sponging miR-124-3p/miR-4524-5p in Hepatocellular Carcinoma
Abstract
:1. Introduction
2. Materials and Methods
2.1. Patient Samples
2.2. Cell Lines
2.3. Cell Transfection
2.4. qRT-PCR Analysis
2.5. Western Blot
2.6. Luciferase Reporter Assay
2.7. circRNAs In Vivo Precipitation (circRIP)
2.8. Statistical Analysis
3. Results
3.1. MRP4 Is Upregulated in HCC
3.2. miR-124-3p Directly Regulates the Expression of MRP4 in HCC
3.3. circHIPK3 Interacted with miR-124-3p and a New Binder miR-4524-5p
3.4. circHIPK3-miR-124-3p/miR-4524-5p Axis Is Involved in MRP4 Upregulation
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Hu, H.; Wang, Y.; Qin, Z.; Sun, W.; Chen, Y.; Wang, J.; Wang, Y.; Nie, J.; Chen, L.; Cai, S.; et al. Regulation of MRP4 Expression by circHIPK3 via Sponging miR-124-3p/miR-4524-5p in Hepatocellular Carcinoma. Biomedicines 2021, 9, 497. https://doi.org/10.3390/biomedicines9050497
Hu H, Wang Y, Qin Z, Sun W, Chen Y, Wang J, Wang Y, Nie J, Chen L, Cai S, et al. Regulation of MRP4 Expression by circHIPK3 via Sponging miR-124-3p/miR-4524-5p in Hepatocellular Carcinoma. Biomedicines. 2021; 9(5):497. https://doi.org/10.3390/biomedicines9050497
Chicago/Turabian StyleHu, Haihong, Yu Wang, Zhiyuan Qin, Wen Sun, Yanhong Chen, Jiaqi Wang, Yingying Wang, Jing Nie, Lu Chen, Sheng Cai, and et al. 2021. "Regulation of MRP4 Expression by circHIPK3 via Sponging miR-124-3p/miR-4524-5p in Hepatocellular Carcinoma" Biomedicines 9, no. 5: 497. https://doi.org/10.3390/biomedicines9050497