Use of MALDI Mass Spectrometry Imaging to Identify Proteomic Signatures in Aortic Aneurysms after Endovascular Repair
Abstract
:1. Introduction
2. Experimental Section
2.1. Patient and Sample Cohort
2.2. MALDI-MSI
2.3. Protein Identification by Electrospray Ionization Tandem Mass Spectrometry
2.4. MALDI-MSI Data Processing for Statistical Analyses
2.5. Statistical Data Analysis
3. Results
3.1. Clinical Characterization
3.2. MALDI-MSI Data
3.3. Discriminative Proteins from TAA, AAA, and EVAR Tissue Sections Based on MALDI-MSI Data
4. Discussion
4.1. Summary
4.2. Impaired Vascular Smooth Muscle Cell Contractility after EVAR
4.3. Increased ECM Degradation after EVAR
4.4. Alteration of the Energy Supply after EVAR
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Subgroup | Gender | Age | Maximum Aneurysm Diameter (mm) | Comorbidities |
---|---|---|---|---|
TAA_1 | M | 62 | 58 | CAD, HI |
TAA_2 | M | 67 | 59 | HI |
TAA_3 | M | 54 | 52 | HI |
TAA_4 | M | 43 | 57 | HI |
TAA_5 | M | 68 | 46 | CAD, HI, AHT, HLP, COPD, CRF |
AAA_1 | M | 78 | 60 | CAD, AHT, HLP |
AAA_2 | M | 72 | 81 | CRF, AHT |
AAA_3 | M | 78 | 80 | CAD, HLP |
AAA_4 | M | 63 | 110 | AHT |
AAA_5 | M | 76 | 68 | CAD, HI, AHT |
EVAR_1 | M | 67 | 75 | CAD, PAD, AHT |
EVAR_2 | M | 64 | 90 | CAD, AHT, HLP |
EVAR_3 | M | 80 | 65 | AF, PAD |
EVAR_4 | M | 73 | 59 | CAD, CRF |
EVAR_5 | M | 65 | 69 | AHT, PAD |
MALDI-MSI m/z Value [Da] | ROC [AUC] Media AAA vs. EVAR | ROC [AUC] Media AAA vs. TAA | ROC [AUC] Media EVAR vs. TAA | LC-MS [MH + Calc.] | Scores | Sequence | Gene Symbol | Protein |
---|---|---|---|---|---|---|---|---|
976.43 | 0.62 | 0.35 | 0.22 | 976.4468194 | 88.56 | AGFAGDDAPR | ACTA2 | Actin, aortic smooth muscle |
1198.65 | 0.66 | 0.29 | 0.10 | 1198.51941 | 57.19 | DSYVGDEAQSK | ||
1198.65 | 0.66 | 0.29 | 0.10 | 1198.703002 | 41.77 | AVFPSIVGRPR | ||
1580.684 | 0.56 | 0.45 | 0.39 | 1580.800411 | 43.04 | MQKEITALAPSTMK | ||
1790.904 | 0.57 | 0.36 | 0.27 | 1790.891339 | 28.06 | SYELPDGQVITIGNER | ||
1564.883 | 0.59 | 0.42 | 0.33 | 1564.905355 | 31.49 | SIVVSPILIPENQR | CDH13 | Cadherin-13 |
1835.908 | 0.58 | 0.44 | 0.34 | 1836.842546 | 32.84 | MTAFDADDPATDNALLR | ||
836.417 | 0.59 | 0.42 | 0.31 | 836.4361428 | 49.08 | GPAGPQGPR | COL1A1 | Collagen alpha-1(I) chain |
852.418 | 0.61 | 0.40 | 0.28 | 851.4249814 | 43.88 | GFSGLDGAK | ||
868.42 | 0.59 | 0.42 | 0.31 | 868.4253882 | 34.63 | GEAGPQGPR | ||
886.421 | 0.56 | 0.43 | 0.36 | 886.4359955 | 47.39 | GSEGPQGVR | ||
784.412 | 0.58 | 0.45 | 0.37 | 785.3889089 | 48.12 | GDQGPVGR | COL1A2 | Collagen alpha-2(I) chain |
1561.883 | 0.60 | 0.48 | 0.37 | 1562.79067 | 87.57 | GETGPSGPVGPAGAVGPR | ||
1237.653 | 0.57 | 0.43 | 0.37 | 1238.651334 | 47.18 | VAVFFSNTPTR | COL6A3 | Collagen alpha-3(VI) chain |
1459.673 | 0.61 | 0.43 | 0.32 | 1459.860756 | 25.39 | IGDLHPQIVNLLK | ||
1462.674 | 0.55 | 0.44 | 0.39 | 1462.763096 | 27.26 | QINVGNALEYVSR | ||
1508.678 | 0.60 | 0.40 | 0.32 | 1507.799786 | 83.26 | LSVEALNSLTGEFK | CKM | Creatine kinase M-type Desmin |
1508.678 | 0.60 | 0.40 | 0.32 | 1507.699725 | 58.36 | GGDDLDPNYVLSSR | ||
1767.902 | 0.57 | 0.47 | 0.39 | 1768.83488 | 32.75 | DGEVVSEATQQQHEVL | ||
2088.931 | 0.56 | 0.45 | 0.39 | 2088.091512 | 51.57 | TFGGAPGFPLGSPLSSPVFPR | ||
853.418 | 0.61 | 0.40 | 0.27 | 853.5233919 | 55.31 | LGPLQVAR | ETFB | Electron transfer flavoprotein subunit beta |
1340.663 | 0.60 | 0.44 | 0.34 | 1339.720602 | 51.07 | LSVISVEDPPQR | ||
974.429 | 0.56 | 0.43 | 0.38 | 973.6021152 | 45.36 | SGVSLAALKK | H1-3 | Histone H1.3 |
1106.641 | 0.58 | 0.42 | 0.32 | 1107.565851 | 47.87 | ALAAAGYDVEK | ||
1198.65 | 0.66 | 0.29 | 0.10 | 1198.666651 | 64.14 | ASGPPVSELITK | ||
1325.661 | 0.59 | 0.44 | 0.36 | 1325.752447 | 45.61 | DNIQGITKPAIR | H4C1 | Histone H4 |
1466.674 | 0.60 | 0.42 | 0.32 | 1466.801839 | 61.03 | TVTAMDVVYALKR | ||
1533.68 | 0.58 | 0.44 | 0.34 | 1533.775171 | 82.04 | VVDSLQTSLDAETR | MYO6 | Myosin-6 |
1850.909 | 0.59 | 0.44 | 0.34 | 1851.041427 | 48.84 | VQLLHSQNTSLINQKK | ||
2088.931 | 0.56 | 0.45 | 0.39 | 2088.123001 | 33.09 | YRILNPVAIPEGQFIDSR | ||
2199.941 | 0.57 | 0.46 | 0.39 | 2200.123705 | 49.68 | GTLEDQIIQANPALEAFGNAK | ||
976.43 | 0.62 | 0.35 | 0.22 | 975.4887038 | 36.63 | AMEAVAAQGK | PGAM2 | Phosphoglycerate mutase 2 |
1150.645 | 0.56 | 0.43 | 0.38 | 1150.666958 | 45.55 | VLIAAHGNSLR | ||
875.42 | 0.57 | 0.44 | 0.37 | 875.4465169 | 30.27 | SLEAQAEK | TPM1 | Tropomyosin alpha-1 chain |
1460.674 | 0.62 | 0.43 | 0.31 | 1460.731208 | 39.4 | KATDAEADVASLNR | ||
1516.679 | 0.61 | 0.43 | 0.32 | 1516.819568 | 27.34 | SKQLEDELVSLQK | ||
1305.659 | 0.58 | 0.41 | 0.34 | 1306.638768 | 44.38 | KNIDALSGMEGR | TNNI3 | Troponin I, cardiac muscle |
1479.675 | 0.54 | 0.43 | 0.40 | 1479.727686 | 42.27 | ISADAMMQALLGAR | ||
1889.913 | 0.57 | 0.46 | 0.38 | 1890.031221 | 44.46 | NITEIADLTQKIFDLR | ||
758.41 | 0.55 | 0.45 | 0.40 | 757.4673223 | 35.39 | ILAERR | TNNT2 | Troponin T, cardiac muscle |
906.423 | 0.55 | 0.43 | 0.37 | 906.5021046 | 26.65 | YEINVLR | ||
1797.904 | 0.57 | 0.46 | 0.40 | 1796.934971 | 29.11 | SFMPNLVPPKIPDGER | ||
1143.445 | 0.60 | 0.42 | 0.31 | 1143.632775 | 28.94 | LAVNMVPFPR | TUBB | Tubulin beta chain |
1320.661 | 0.64 | 0.38 | 0.24 | 1319.701066 | 49.82 | IMNTFSVVPSPK | ||
1269.656 | 0.56 | 0.43 | 0.37 | 1270.559399 | 37.39 | LGDLYEEEMR | VIM | Vimentin |
1428.671 | 0.55 | 0.33 | 0.28 | 1428.710851 | 40.41 | SLYASSPGGVYATR | ||
2498.168 | 0.55 | 0.49 | 0.44 | 2497.256473 | 42.86 | LLQDSVDFSLADAINTEFKNTR |
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Buerger, M.; Klein, O.; Kapahnke, S.; Mueller, V.; Frese, J.P.; Omran, S.; Greiner, A.; Sommerfeld, M.; Kaschina, E.; Jannasch, A.; et al. Use of MALDI Mass Spectrometry Imaging to Identify Proteomic Signatures in Aortic Aneurysms after Endovascular Repair. Biomedicines 2021, 9, 1088. https://doi.org/10.3390/biomedicines9091088
Buerger M, Klein O, Kapahnke S, Mueller V, Frese JP, Omran S, Greiner A, Sommerfeld M, Kaschina E, Jannasch A, et al. Use of MALDI Mass Spectrometry Imaging to Identify Proteomic Signatures in Aortic Aneurysms after Endovascular Repair. Biomedicines. 2021; 9(9):1088. https://doi.org/10.3390/biomedicines9091088
Chicago/Turabian StyleBuerger, Matthias, Oliver Klein, Sebastian Kapahnke, Verena Mueller, Jan Paul Frese, Safwan Omran, Andreas Greiner, Manuela Sommerfeld, Elena Kaschina, Anett Jannasch, and et al. 2021. "Use of MALDI Mass Spectrometry Imaging to Identify Proteomic Signatures in Aortic Aneurysms after Endovascular Repair" Biomedicines 9, no. 9: 1088. https://doi.org/10.3390/biomedicines9091088