Synthesis, In Silico, and In Vitro Biological Evaluation of New Furan Hybrid Molecules
Abstract
:1. Introduction
2. Materials and Methods
2.1. Synthesis
2.2. Biological Evaluation
2.3. Biological Experiments
2.3.1. Hydrogen Peroxide Scavenging Activity (HPSA)
2.3.2. Metal-Chelating Activity (MChA) on Ferrous Ions
2.3.3. Inhibition of Albumin Denaturation (IAD)
2.3.4. Antitryptic Activity (ATA)
2.4. Physicochemical Characterization
2.4.1. Determination of Lipophilicity as RM Values
2.4.2. Prediction of Anti-Inflammatory and Anti-Arthritic Activity
2.5. Statistical Analysis
3. Results
3.1. Synthesis
3.2. Biological Evaluation
3.2.1. Hydrogen Peroxide Scavenging Activity
3.2.2. Metal-Chelating Activity (MChA) on Ferrous Ions
3.2.3. Inhibition of Albumin Denaturation (IAD)
3.2.4. Antitryptic Activity (ATA)
3.2.5. Lipophilicity
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Compounds | IC50± SD, μg/mL | IC50± SD, mg/mL | RM ± SD | Pa | |||
---|---|---|---|---|---|---|---|
HPSA | IAD | ATA | MChA | cAnti-I | cAnti-A | ||
AA | 24.84 ± 0.35 | - | - | - | - | - | - |
Qrc | 69.25 ± 1.82 | - | - | - | - | - | - |
Ibu | - | 81.50 ± 4.95 | 259.82 ± 9.14 | - | 1.11 ± 0.010 | 0.903 | 0.573 |
Ket | - | 126.58 ± 5.00 | 720.57 ± 19.78 | - | 1.54 ± 0.015 | 0.925 | 0.469 |
H1 | 123.33 ± 7.41 | 114.31 ± 2.88 | 83.25 ± 1.69 | 4.92 ± 0.89 | 1.04 ± 0.023 | 0.224 | - |
H2 | 105.52 ± 10.33 | 116.76 ± 1.61 | 60.21 ± 8.16 | 4.26 ± 0.79 | 0.94 ± 0.016 | 0.226 | - |
H3 | 77.75 ± 0.67 | 150.99 ± 1.16 | 85.33 ± 7.26 | 1.02 ± 0.12 | 1.01 ± 0.030 | - | - |
H4 | 71.72 ± 4.63 | 119.08 ± 0.92 | 62.23 ± 0.83 | 1.46 ± 0.06 | 0.87 ± 0.015 | 0.201 | - |
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Manolov, S.; Ivanov, I.; Bojilov, D.; Nedialkov, P. Synthesis, In Silico, and In Vitro Biological Evaluation of New Furan Hybrid Molecules. Processes 2022, 10, 1997. https://doi.org/10.3390/pr10101997
Manolov S, Ivanov I, Bojilov D, Nedialkov P. Synthesis, In Silico, and In Vitro Biological Evaluation of New Furan Hybrid Molecules. Processes. 2022; 10(10):1997. https://doi.org/10.3390/pr10101997
Chicago/Turabian StyleManolov, Stanimir, Iliyan Ivanov, Dimitar Bojilov, and Paraskev Nedialkov. 2022. "Synthesis, In Silico, and In Vitro Biological Evaluation of New Furan Hybrid Molecules" Processes 10, no. 10: 1997. https://doi.org/10.3390/pr10101997