Determination of Ten Plant Growth Regulators in Bean Sprouts by Mixed Solid Phase Extraction Columns Separation and LC-MS/MS
Abstract
:1. Introduction
2. Results and Discussion
2.1. LC-MS/MS Method Development
2.2. Accuracy, Precision, and Quality Control of the Developed Method
2.3. Sample Analysis
3. Materials and Methods
3.1. Reagents and Instruments
3.2. Preparation of Standard Solutions and Reagents
3.3. Sample Preparation
3.3.1. Sample Extraction
3.3.2. Purification
3.4. LC-MS/MS Conditions
3.5. Method Validation
3.5.1. Standard Curve Preparation
3.5.2. Accuracy, Precision, and Recovery
3.5.3. Limit of Detection and Limit of Quantification
3.6. Calculation
- X = concentration of PRGs in the sample (mg/kg);
- C = concentration of PRGs in the prepared sample solutions (ng/mL);
- m = mass of bean sprout samples subject to extraction (g);
- 2 = volume of MeCN extract taken for purification (mL);
- 20 = volume of MeCN used to extract the bean sprout samples (mL);
- 1000 = the dilution factor
- All the data were average values from three trials.
3.7. Statistical Analysis
4. Conclusions
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Chemicals | Retention Time (min) | Quantitative Ions * | Qualitative Ions * | Ratio of Quantitative to Qualitative Ions |
---|---|---|---|---|
IAA | 2.52 | 176.3 > 130.1 (10, 24) | 176.3 > 103.0 (10, 28) | 0.659 |
GA3 | 2.64 | 345.1 > 143.1 (15, 35) | 345.1 > 239.1.1 (15, 20) | 0.695 |
4-CPA | 2.83 | 185.0 > 127.0 (15, 15) | 187.0 > 129.0 (15, 10) | 0.193 |
IBA | 2.94 | 204.0 > 130.1 (24, 40) | 204.0 > 186.0 (24, 25) | 0.194 |
6-KT | 3.01 | 216.0 > 81.0 (2, 12) | 216.0 > 148.0 (2, 16) | 0.243 |
2,4-D | 3.02 | 218.9 > 160.9 (10, 18) | 218.9 > 124.9 (10, 35) | 0.028 |
6-BA | 3.24 | 224.0 > 133.1 (15, 36) | 224.0 > 106.2 (15, 21) | 0.056 |
TDZ | 3.54 | 219.1 > 100.1 (8, 13) | 219.1 > 71.2 (8, 35) | 0.095 |
FCF | 3.81 | 246.0 > 127.0 (10, 15) | 246.0 > 91.0 (10, 35) | 0.065 |
PBZ | 4.10 | 294.0 > 70.0 (10, 25) | 294.0 > 124.9 (10, 40) | 0.142 |
Chemicals | Standard Spiked (ng/g) | Average Recovery (%) | RSD% | LOQ (ng/g) | LOD (ng/g) |
---|---|---|---|---|---|
GA3 | 5 | 68.3 | 15.2 | 5 | 2 |
50 | 81.2 | 9.7 | 5 | 2 | |
500 | 86.4 | 6.4 | 5 | 2 | |
2,4-D | 5 | 68.3 | 15.2 | 5 | 2 |
50 | 74.6 | 11.7 | 5 | 2 | |
500 | 90.5 | 7.2 | 5 | 2 | |
4-CPA | 5 | 75.7 | 10.6 | 5 | 2 |
50 | 88.6 | 9.1 | 5 | 2 | |
500 | 95.3 | 5.8 | 5 | 2 | |
IBA | 5 | 73.1 | 14.2 | 5 | 2 |
50 | 78.8 | 10.8 | 5 | 2 | |
500 | 84.2 | 8.2 | 5 | 2 | |
IAA | 5 | 74.3 | 13.4 | 5 | 2 |
50 | 80.2 | 8.4 | 5 | 2 | |
500 | 83.7 | 6.3 | 5 | 2 | |
FCF | 5 | 80.3 | 9.2 | 5 | 2 |
50 | 83.4 | 7.6 | 5 | 2 | |
500 | 89.3 | 5.2 | 5 | 2 | |
TDZ | 5 | 76.3 | 11.3 | 5 | 2 |
50 | 79.3 | 9.4 | 5 | 2 | |
500 | 83.4 | 6.1 | 5 | 2 | |
6-BA | 5 | 78.6 | 9.3 | 5 | 2 |
50 | 82.5 | 8.4 | 5 | 2 | |
500 | 91.3 | 5.6 | 5 | 2 | |
PBZ | 5 | 83.4 | 10.3 | 5 | 2 |
50 | 86.4 | 7.8 | 5 | 2 | |
500 | 97.3 | 4.6 | 5 | 2 | |
6-KT | 5 | 77.8 | 12.4 | 5 | 2 |
50 | 81.2 | 9.1 | 5 | 2 | |
500 | 89.1 | 6.7 | 5 | 2 |
Chemicals | Content Range (mg/kg) | Soybean Sprout Detection Rate (%) | Mungbean Sprouts Detection Rate (%) | Total Detection Rate (%) |
---|---|---|---|---|
Gibberellin | <LOD | / | / | / |
2,4-D | <LOD | / | / | / |
4-CPA | 0.02–0.26 * | 19.4 (7/36) | 15.2 (5/33) | 17.4 (12/69) |
IBA | <LOD | / | / | / |
IAA | 0.04–2.5 * | 11.1 (4/36) | 27.3 (9/33) | 18.8 (13/69) |
FCF | <LOD | / | / | / |
TDZ | <LOD | / | / | / |
6-BA | <LOD | / | / | / |
PBZ | <LOD | / | / | / |
6-KT | <LOD | / | / | / |
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Cai, C.; Yao, F.; Li, C.; Xiang, Y.; Wu, P.; Hu, Z.; Wang, J.; Zhu, R. Determination of Ten Plant Growth Regulators in Bean Sprouts by Mixed Solid Phase Extraction Columns Separation and LC-MS/MS. Processes 2023, 11, 2586. https://doi.org/10.3390/pr11092586
Cai C, Yao F, Li C, Xiang Y, Wu P, Hu Z, Wang J, Zhu R. Determination of Ten Plant Growth Regulators in Bean Sprouts by Mixed Solid Phase Extraction Columns Separation and LC-MS/MS. Processes. 2023; 11(9):2586. https://doi.org/10.3390/pr11092586
Chicago/Turabian StyleCai, Chenggang, Feng Yao, Chuanpeng Li, Yannan Xiang, Pinggu Wu, Zhengyan Hu, Junlin Wang, and Ruiyu Zhu. 2023. "Determination of Ten Plant Growth Regulators in Bean Sprouts by Mixed Solid Phase Extraction Columns Separation and LC-MS/MS" Processes 11, no. 9: 2586. https://doi.org/10.3390/pr11092586