Supercritical CO₂-Based Extraction and Detection of Phenolic Compounds and Saponins from the Leaves of Three Medicago varia Mart. Varieties by Tandem Mass Spectrometry

Round 1

Reviewer 1 Report

Comments and Suggestions for Authors

The manuscript seems interesting as apparently new species of alfalfa have been compared and characterized by HPLC-MS. However, minor changes should be done before publishing.

 

Please check carefully words spelling and English.

 

Introduction:

-If doing this statement “ but also in expanding the raw 39 material base of the food, cosmetic and pharmaceutical industries.” Include the references that corroborate that alfalfa is used in cosmetic and pharmaceutical.

-Please, highlight the novelty of the research.

Materials and methods:

-2.3., 2.4 and 2.5 include references

 

Results:

-“The maximum yield of bioactive substances 114 from alfalfa aerial parts (variety Sarga) was observed under the following extraction condition:” If these two conditions are mentioned, please include in materials all the conditions tested. It it was performed a quadratic model, please include this data.

-Line 169: The table mentioned does not match with the number of the following table.

-I would appreciate if authors focused a little more on the extraction process, and also in the discussion section.

Best regards

Author Response

Dear Reviewer

The team of authors would like to thank you for considering our scientific article.

Having considered and taken into account all your comments, we have corrected as much as possible all the inaccuracies and errors found in the article. We also significantly added to the text of the article. It is highlighted in blue.
Additionally, the number of cited articles increased, reaching 143.

We hope for your positive attitude and are ready to cooperate in every possible way.

Sincerely yours

 

Author Response File: Author Response.pdf

Reviewer 2 Report

Comments and Suggestions for Authors

The authors must implement the introduction, with indexed bibliographical references; in this part the references are not entirely appropriate.

There are some inaccuracies in the text, please correct.

What is the extraction yield?

The research identified new compounds not yet identified in alfalfa, as indicated in the conclusions.

Correct the subscripts in all CO2 terms.

Indicate the names of the species in italics.

Author Response

Dear Reviewer

The team of authors would like to thank you for considering our scientific article.

Having considered and taken into account all your comments, we have corrected as much as possible all the inaccuracies and errors found in the article. We also significantly added to the text of the article. It is highlighted in blue.
Additionally, the number of cited articles increased, reaching 143.

We hope for your positive attitude and are ready to cooperate in every possible way.

Sincerely yours

 

Author Response File: Author Response.pdf