Assessment of the Release Profile of Fibroblast Growth Factor-2-Load Mesoporous Calcium Silicate/Poly-ε-caprolactone 3D Scaffold for Regulate Bone Regeneration
Abstract
:1. Introduction
2. Materials and Methods
2.1. CS Mesoporous Nanoparticles Synthesis
2.2. FGF-2 Loading
2.3. MCS and MCSF Scaffold Fabrication
2.4. FGF-2 Release and Bioactivity
2.5. Cell Proliferation
2.6. Western Blot
2.7. ALP Activity
2.8. Rabbit Model of Femoral Bone Defects
2.9. Microcomputed Tomography
2.10. Histological Staining
2.11. Statistical Analyses
3. Results and Discussion
3.1. Characterization of the MCSF/PCL Scaffold
3.2. In Vitro Soaking
3.3. In Vitro Experiments
3.4. In Vivo Experiments
4. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Kao, C.-T.; Chen, Y.-J.; Huang, T.-H.; Lin, Y.-H.; Hsu, T.-T.; Ho, C.-C. Assessment of the Release Profile of Fibroblast Growth Factor-2-Load Mesoporous Calcium Silicate/Poly-ε-caprolactone 3D Scaffold for Regulate Bone Regeneration. Processes 2020, 8, 1249. https://doi.org/10.3390/pr8101249
Kao C-T, Chen Y-J, Huang T-H, Lin Y-H, Hsu T-T, Ho C-C. Assessment of the Release Profile of Fibroblast Growth Factor-2-Load Mesoporous Calcium Silicate/Poly-ε-caprolactone 3D Scaffold for Regulate Bone Regeneration. Processes. 2020; 8(10):1249. https://doi.org/10.3390/pr8101249
Chicago/Turabian StyleKao, Chia-Tze, Yen-Jen Chen, Tsui-Hsien Huang, Yen-Hong Lin, Tuan-Ti Hsu, and Chia-Che Ho. 2020. "Assessment of the Release Profile of Fibroblast Growth Factor-2-Load Mesoporous Calcium Silicate/Poly-ε-caprolactone 3D Scaffold for Regulate Bone Regeneration" Processes 8, no. 10: 1249. https://doi.org/10.3390/pr8101249