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Review

Effect of Oxaliplatin on Voltage-Gated Sodium Channels in Peripheral Neuropathic Pain

by
Woojin Kim
Department of Physiology, College of Korean Medicine, Kyung Hee University, Seoul 02453, Korea
Processes 2020, 8(6), 680; https://doi.org/10.3390/pr8060680
Submission received: 6 May 2020 / Revised: 29 May 2020 / Accepted: 6 June 2020 / Published: 9 June 2020
(This article belongs to the Special Issue Cancer Systems Biology and Natural Products)
Versions Notes

Abstract

:
Oxaliplatin is a chemotherapeutic drug widely used to treat various types of tumors. However, it can induce a serious peripheral neuropathy characterized by cold and mechanical allodynia that can even disrupt the treatment schedule. Since the approval of the agent, many laboratories, including ours, have focused their research on finding a drug or method to decrease this side effect. However, to date no drug that can effectively reduce the pain without causing any adverse events has been developed, and the mechanism of the action of oxaliplatin is not clearly understood. On the dorsal root ganglia (DRG) sensory neurons, oxaliplatin is reported to modify their functions, such as the propagation of the action potential and induction of neuropathic pain. Voltage-gated sodium channels in the DRG neurons are important, as they play a major role in the excitability of the cell by initiating the action potential. Thus, in this small review, eight studies that investigated the effect of oxaliplatin on sodium channels of peripheral neurons have been included. Its effects on the duration of the action potential, peak of the sodium current, voltage–response relationship, inactivation current, and sensitivity to tetrodotoxin (TTX) are discussed.

1. Introduction

Oxaliplatin is a third-generation platinum-based chemotherapeutic drug that is widely used to treat various types of cancer, such as breast, colorectal, and lung cancers [1]. Although it is an effective drug as it does not induce significant ototoxicity and nephrotoxicity like other platinum derivatives, it has a serious side effect, which is characterized by paresthesia and dysesthesia of hands and feet, which can often lead to the discontinuation of therapy [2]. Hence, understanding the mechanism of action and finding an effective treatment for oxaliplatin-induced neuropathic pain are critical issues for treatment, and for the patients’ quality of life.
Although the mechanism underlying oxaliplatin-induced neuropathic pain remains unclear, mechanistic studies have reported various pathways. Sodium, potassium, and calcium ion channels and different types of transient receptor potential family (TRPA1, TRPM8, and TRPV1) were shown to be involved, as their function altered after oxaliplatin injection [3,4,5,6,7]. In other studies, increased activity of spinal glia cells and pro-inflammatory and neuroexcitatory cytokines (tumor necrosis factor alpha and interleukin-1beta) in the dorsal horn of the spinal cord were associated with oxaliplatin [8,9]. Furthermore, environmental changes owing to oxaliplatin administration, such as toxic effects on mitochondria [10] and pH acidification [11] in the dorsal root ganglia (DRG) sensory neurons have also been suggested as the cause of allodynia and hyperexcitability.
For several years, we have focused on finding an effective method to decrease this pain. Using an animal model of oxaliplatin-induced neuropathic pain, which showed similar symptoms to patients, such as cold and mechanical allodynia [12,13,14], we have reported that various treatments with well-known drugs, such as morphine [13] and duloxetine [15], and alternative methods [16], such as using acupuncture [17] and bee venom [13,18,19], can significantly attenuate allodynia.
Furthermore, by using in vivo spinal cord extracellular recording methods, we demonstrated that the activities of wide dynamic range (WDR) neurons, which are present in the spinal dorsal horn and encode the information received from both the innocuous and noxious signs that transmit peripheral neurons [20], are highly upregulated after oxaliplatin treatment compared to that after vehicle injection in rats [15,16,18]. These results let us focus on the role of peripheral sensory neurons in oxaliplatin-induced neuropathic pain [21]. The function of DRG sensory neurons, such as conduction velocity, was affected after administration of the chemotherapeutic agent. However, the number of cells remained unchanged, as did central and motor nerve conduction, suggesting that the agent mostly affects the function of the sensory neurons [21].
DRG sensory neurons play a major role in pain sensation, and their excitability is closely related to the propagation of action potentials, which transmit noxious information to the central nervous system [22]. Oxaliplatin is known to induce an immediate effect on the axonal excitability rather than structural changes, suggesting that ion channels in the DRG neurons may be involved [23]. Indeed, abnormalities in the functions of potassium and calcium ion channels were reported as the expression of pro-excitatory channels (hyperpolarization-activated channels) [24] and calcium ion influx were significantly increased following oxaliplatin injection, respectively. However, as the membrane potential of a neuron depends mostly on multiple isoforms of a voltage-gated sodium channel and the function of the sodium channels in primary DRG neurons can determine the occurrence and development of neuropathic pain, sodium channels may play a key role in oxaliplatin-induced neuropathic pain [25,26]. Moreover, administration of sodium channel blockers was reported to be effective against oxaliplatin-induced neuropathic pain in humans [27,28] and animals [29,30,31], suggesting that concentrating on the voltage-gated sodium channel may be an effective treatment approach.
Since the study published by Adelsberger et al. [31], seven more studies have investigated the effect of oxaliplatin on voltage-gated sodium channels, during the past 20 years. Thus, in this review, we included all eight studies. Clinical trials were excluded, and in vitro and in vivo studies conducted with animals were included. Animal species and nerves investigated all differed from paper to paper. Three used rats and the other three studies used mice. In addition, frogs, cockroaches, and human nerves were also analyzed in other studies.
Considering the importance of the peripheral sensory neurons and voltage-gated sodium channels in the development and maintenance of neuropathic pain, it appeared to be important to combine all studies published during the past 20 years, and report on them for a better understanding of the action of oxaliplatin and the development of an effective drug (Table 1).

2. Effect of Oxaliplatin on Peripheral Nerve Fibers

2.1. Oxaliplatin Mostly Affects Myelinated A-Fibers, but Not Unmyelinated C-Fibers

Adelsberger et al. [31] conducted an experiment on the sural, peroneal, and vagal nerves of adult rats, by evoking a stimulus of 100 V for 15 μs in rat sural nerves. They demonstrated that the A-fiber compound action potential (CAP) increased significantly (218 ± 68%, n = 7). In contrast, the C-fiber CAP did not change significantly (36 ± 29%, n = 7) after 45 min of 250 μM oxaliplatin treatment, showing that oxaliplatin mostly affects A rather than C-fibers. This change in A-fiber was completely blocked by 1 mM of a sodium channel blocker, carbamazepine. Following Adelsberger et al., Sittl et al. [32] also reported that oxaliplatin affected only myelinated A-fibers but not C-fibers. An electrical stimulation in the human nerve fascicle (from five male patients, 57 ± 9 years of age) bathed in 100 μM of oxaliplatin for 90 min induced after-potentials in the A but not C-fiber CAP. In addition, in the sural nerves of wild-type mice, the stimulus evoked repetitive action potential discharge only in myelinated fibers, and action potentials increased upon cooling the media to 20 °C.

2.2. Oxaliplatin Increases the Length of the Action Potential

By stimulating the rat sural nerve with twin pulse action potentials, Adelsberger et al. [31] reported that 250 μM of oxaliplatin application increased both the amplitude and duration of action potentials. In addition, the time needed for an action potential to reach the baseline increased dramatically after 45 min of chemotherapeutic drug application, from 500 μs to 10 ms. These effects were induced by lengthening the refractory period. Grolleau et al. [33] also demonstrated that 500 μM of intracellularly-applied oxaliplatin reduced the spike amplitude by 19.4%. However, it increased the action potential duration while reducing both the depolarizing phase and post-hyperpolarization. In their experiment, action potentials were evoked by 50 ms of 0.8 nA depolarizing current-pulse stimulation in cockroach dorsal unpaired median (DUM) neurons. However, with their experiments conducted on the myelinated frog neurons, Benoit et al. [34] suggested that oxaliplatin may decrease the duration of action potentials, as in their experiments, the voltage-dependence of sodium channel inactivation moved towards negative. Although Alberti et al. [35] did not mention the duration of the action potential, they showed that intravenous administration of oxaliplatin decreased the refractory period compared to the control (control: 0.365 vs. oxaliplatin: 0.276 ms, respectively, P = 0.02), suggesting that the length of the action potential may have shortened.

3. Oxaliplatin Modifies Activities of Voltage-Gated Sodium Channels

3.1. Oxaliplatin Decreases the Peak of the Sodium Current

In their experiment, Adelsberger et al. [31] reported that 250 μM of oxaliplatin decreased the maximal amplitude of the sodium current to 0.65 ± 0.23 in 13 out of 18 cells tested. In DUM neurons, Grolleau et al. [33] also reported that 20 min extracellular application of 100 μM of oxaliplatin induced a slight reduction in the maximum peak sodium current (16.7 ± 5.8% of control). In contrast, the intracellular treatment at the same dose and time inhibited the current highly (52.8 ± 3.3% of control). This effect of intracellular treatment was observed in seven of the eleven treated cells. They further conducted experiments with dichlorodiaminocyclohexane platinum (Dach-Cl2-platin) and oxalate, which are the two major metabolites of oxaliplatin [36]. They found that oxalate, but not Dach-Cl2-platin, could decrease the maximum sodium current peak similarly to oxaliplatin. When 500 μM of oxalate was administered intracellularly, the sodium current decreased by 36 ± 2% of the control. These results suggested that oxalate may play an important role in the effect of oxaliplatin. In accordance with previously reported results, Benoit et al. [34] showed that the peak sodium current consistently decreased to 77 ± 8% and 41 ± 7% of its control value after 10 μM and 100 μM oxaliplatin application, respectively.

3.2. Oxaliplatin Shifts the Voltage–Response Relationship of the Sodium Current

Adelsberger et al. [31] reported that 250 μM of oxaliplatin shifted the voltage–response relationship of 10 mV toward more negative potentials. Benoit et al. [34] reported that oxaliplatin shifted the peak sodium current voltage curves toward negative membrane potentials, showing that oxaliplatin induces current activation at a more negative potentials. Under the chemotherapeutic agent, the sodium current started to be activated at −50 mV with 10 or 100 μM, whereas no current was active under control conditions.

3.3. Oxaliplatin Slowdown Inactivation Kinetics of Voltage-Gated Sodium Channels

On the effect of oxaliplatin on the inactivation kinetics, there is conflicting evidence that whether oxaliplatin induces a slowdown of the inactivation current. First, Adelsberger et al. [31] reported that the slowdown of the inactivation kinetics of the channel occurred after oxaliplatin injection, and that this slowdown of the inactivation sodium current resulted in an increase in the total current by 2.6 ± 1.45%. This effect could be observed only in the DRG and not in the hippocampal neurons, suggesting that oxaliplatin preferentially acts on certain types of voltage-gated sodium channel isoforms expressed mainly in the peripheral nervous system. Sittl et al. [32] also demonstrated that oxaliplatin significantly induced slow inactivation at a negative potential in isolation in neuron-derived ND7/23 cells transfected with the murine Nav1.6r and auxiliary β4-subunit. The slowing inactivation at a negative membrane potential prolonged Nav open times and increased resurgent and persistent current in native DRG neurons. However, in experiments conducted on frog myelinated neurons, Benoit et al. [34] reported that oxaliplatin did not induce slow down inactivation of the sodium current. This discrepancy may be due to the difference in the animals used (rat DRG and cockroach DUM neurons vs. frog sciatic nerve).

4. Effect of Oxaliplatin on TTX-Sensitive or Resistant Sodium Channels

4.1. Oxaliplatin Modulates the Activity of TTX-Sensitive Sodium Channels

DRG neurons express different types of voltage-gated sodium channel isoforms: TTX-resistant isoforms Nav1.8 and Nav1.9, and TTX-sensitive isoforms Nav1.1, Nav1.2, Nav1.3, Nav1.6, and Nav1.7 [37]. To investigate whether oxaliplatin affects TTX-sensitive or TTX-resistant voltage-gated sodium channels, Webster et al. [29] evoked potential in the phrenic nerve hemidiaphragm and vas deferens to evaluate the effect of oxaliplatin on both the motor nerve function and autonomic system. They demonstrated that more than 30 min of 0.5 mM of oxaliplatin application induced increased endplate potential (EPP), miniature endplate potentials (MEPPs), and frequency of spontaneous excitatory junction potential (sEJP). However, these effects were all blocked by 1 μM of TTX, showing that oxaliplatin modifies the activity of TTX-sensitive voltage-gated sodium channels. Furthermore, in accordance with the data of Adelsberger et al. [31], 0.3 mM of sodium channel blocker, carbamazepine, also prevented the multiple EPPs and increased spontaneous activities.

4.2. Effect of Oxaliplatin on Nav1.6

Nav1.6 is a TTX-sensitive voltage-gated sodium channel expressed in various types of neuronal cells. Studies have shown evidence that Nav1.6 is expressed both in the central and peripheral nervous system, but mostly on myelinated axons [25,38]. Sittl et al. [32] reported that after potentials induced by oxaliplatin were completely absent in Nav1.6 knockout mice, but not in heterozygous mice. Moreover, by using Nav1.6 deficient mice (Scn8amed/med), they showed that cooling the media to 20 °C induced stimulus evoked action potential bursts in single axons, whereas these action potentials could not be observed on physiological skin temperature (i.e., 30–32 °C). As reported by Sittl et al. [32], Deuis et al. [39] also showed that Nav1.6 plays an important role in the activity of oxaliplatin. In their experiment, Nav1.8-/- and 1.9-/- mice as well as Nav1.8-selective small-molecule inhibitor’s application showed oxaliplatin-induced cold allodynia, suggesting that TTX-sensitive, but not resistant voltage-gated sodium channels play an important role in the development of allodynia. To prove this, they injected a low dose of TTX and demonstrated that TTX can indeed inhibit cold allodynia. Subsequently, to determine which TTX-sensitive sodium channel subtype plays a major role in the development of pain, they conducted experiments with Nav1.3-/- and Nav1.7, 1.1, 1.2, and 1.4 subtype selective inhibitors (ProTxII and μ-Conotoxin TIIIA, respectively), but none of these inhibitors affected the cold allodynia induced by oxaliplatin. However, intraplantar injection of 10 μM GIIIA, which, in addition to Nav1.1, also inhibits Nav1.6 at high concentrations [40], achieved near-complete reversal of oxaliplatin-induced cold allodynia (14 ± 9% of control), demonstrating the functional contribution of Nav1.6 to cold pain pathways at the behavioral level. Li et al. [30] showed that following intraperitoneal oxaliplatin injection (2.4, 3.2, or 4.0 mg/kg twice weekly for 4.5 weeks), expression levels of Nav1.6 mRNA (F(3,15) = 60.98, P < 0.0001), and protein (F(3,15) = 15.00, P = 0.0002) were significantly increased compared to naïve rats. Moreover, the expression of Nav1.6 (F(3,11) = 18.67, P = 0.0006, and F(3,11) = 52.54, P < 0.0001) in the DRG was also markedly upregulated within each dose of oxaliplatin. Furthermore, immunofluorescent staining showed a significant increase in the ratio of Nav1.6-positive DRG neurons in oxaliplatin-treated rats compared to control rats (P < 0.001). These results provide strong evidence indicating that oxaliplatin alters the expression of TTX-sensitive Nav1.6 in DRG neurons.

5. Conclusions and Perspectives

In this review, the effect of oxaliplatin on voltage-gated sodium channels was observed based on the results obtained from eight studies. Oxaliplatin was shown to affect myelinated A-fibers more compared to unmyelinated C-fibers, as CAP induced in C-fiber did not change following oxaliplatin injection [31,32]. Although in these studies, the subtypes of A-fibers affected by oxaliplatin were not mentioned, it has been reported that Aδ-fibers are affected by oxaliplatin [41]. Aδ-fibers, which are sensory myelinated fibers with the thinnest myelin sheaths, are related to paresthesias (tickling or tingling sensations in the skin, whether distal or perioral), which is one of the most frequent symptoms of oxaliplatin-treated patients [42]. In addition, by using quantitative sensory testing, it was reported that cold pain are served by Aδ-fibers [43], and a clinical study demonstrated that blockade of A-fibers reduced the cold hyperalgesia in oxaliplatin treated patients [44].
In addition, two studies reported that oxaliplatin increased the duration of the action potential [31,33], while other data showed that the duration of the action potential was reduced [34]. However, as mentioned above, these discrepancies may result from differences in the animals (rat DRG and cockroach DUM neurons vs. frog sciatic nerve) or the doses used (250 and 500 μM vs. 10 and 100 μM). In contrast, three studies concur that oxaliplatin decreased the peak of the sodium current [31,33,34], as also reported in a clinical trial [45]. In this review, the precise mechanism has not been mentioned; however, Benoit et al. [34] and Grolleau et al. [33] mentioned that oxalate, which is a major metabolite of oxaliplatin, may act as a calcium ion chelator, blocking the voltage-gated sodium channel by controlling the external surface membrane potentials [33,34,46].
The importance of calcium ions in oxaliplatin-induced allodynia was reported in several articles [47,48], and some clinical studies have recommended calcium and magnesium infusion to prevent oxaliplatin-induce neuropathic pain [49,50]. Indeed, in the experiments of Deuis et al. [39], intraplantar injection of BAPTA (1,2-bis(o-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid), which is a calcium ion chelator, mimicked the allodynia induced by oxaliplatin, showing that removal of the extracellular calcium ion plays an important role in neuropathic pain. BAPTA injection decreased the threshold potential and membrane resistance, producing effects on inward sodium currents. As a divalent cation, calcium ion is able to modify the properties of the voltage-gated sodium channel by hyperpolarizing the cell membrane; thus, increased extracellular calcium ion concentration may induce sodium channel closing and reduce the effect of oxaliplatin on the activity of sodium channels.
Most studies included in the review reported that oxaliplatin acts on the TTX-sensitive pathway [29,32,39]. DRG neurons express several voltage-gated sodium channel isoforms, including the TTX-resistant isoforms Nav1.8 and Nav1.9, and the TTX-sensitive isoforms Nav1.1, Nav1.2, Nav1.3, Nav1.6, and Nav1.7 [37]. In the experiments of Deuis et al. [39], neither TTX-resistant sodium channel knockout mice nor inhibitor injection altered the pain induced by oxaliplatin, and among the various subtypes of TTX-resistant sodium channel, Nav1.6 was shown to be the most affected by oxaliplatin, as inhibition of this channel led to attenuation of cold allodynia. Voltage-gated sodium channels play a central role in the development of pain, as they are important for the initial transduction of sensory stimuli, the electrogenesis of the action potential, and neurotransmitter release from sensory neuron terminals [51]. Clinical trials have reported that carbamazepine, an anticonvulsant and a voltage-dependent sodium channel blocker, could attenuate oxaliplatin-induced neuropathic pain [27,52,53]. Duloxetine, a well-established anticonvulsants and analgesic drug, is recommended by the American Society of Clinical Organization (ASCO) guidelines [54] and the Journal of the American Medical Association (JAMA) [55] to prevent neuropathic pain induced by oxaliplatin. Duloxetine is a serotonin and norepinephrine reuptake inhibitor (SNRI); however, in addition to potentiation of serotonergic and noradrenergic activity in the central nervous system, duloxetine has been shown to be able to block neuronal sodium ion currents for its efficacy in neuropathic pain [56,57]. These studies show that duloxetine may decrease oxaliplatin-induced pain by modulating voltage-gated sodium channels.
In this review, only the effects of oxaliplatin on voltage-gated sodium channels have been noted. However, for a better understanding of the mechanism, the simultaneous effects of oxaliplatin and its metabolites on voltage-gated sodium channels and other ion channels, such as potassium and calcium ion channels, should be investigated. Furthermore, the effects of these ion channel blockers’ co-administration should also be evaluated, as the activities of these ion channels are closely related to each other.

Funding

This work was supported by a grant from Kyung Hee University in 2018 (KHU-20181294).

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Misset, J.L.; Bleiberg, H.; Sutherland, W.; Bekradda, M.; Cvitkovic, E. Oxaliplatin clinical activity: A review. Crit. Rev. Oncol. Hematol. 2000, 35, 75–93. [Google Scholar] [CrossRef]
  2. Argyriou, A.A.; Polychronopoulos, P.; Iconomou, G.; Chroni, E.; Kalofonos, H.P. A review on oxaliplatin-induced peripheral nerve damage. Cancer Treat. Rev. 2008, 34, 368–377. [Google Scholar] [CrossRef] [PubMed]
  3. Nativi, C.; Gualdani, R.; Dragoni, E.; Mannelli, L.D.C.; Sostegni, S.; Norcini, M.; Gabrielli, G.; La Marca, G.; Richichi, B.; Francesconi, O. A trpa1 antagonist reverts oxaliplatin-induced neuropathic pain. Sci. Rep. 2013, 3, 2005. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  4. Nassini, R.; Gees, M.; Harrison, S.; De Siena, G.; Materazzi, S.; Moretto, N.; Failli, P.; Preti, D.; Marchetti, N.; Cavazzini, A. Oxaliplatin elicits mechanical and cold allodynia in rodents via trpa1 receptor stimulation. Pain® 2011, 152, 1621–1631. [Google Scholar] [CrossRef] [PubMed]
  5. Zhao, M.; Isami, K.; Nakamura, S.; Shirakawa, H.; Nakagawa, T.; Kaneko, S. Acute cold hypersensitivity characteristically induced by oxaliplatin is caused by the enhanced responsiveness of trpa1 in mice. Mol. Pain 2012, 8, 55. [Google Scholar] [CrossRef] [Green Version]
  6. Descoeur, J.; Pereira, V.; Pizzoccaro, A.; Francois, A.; Ling, B.; Maffre, V.; Couette, B.; Busserolles, J.; Courteix, C.; Noel, J. Oxaliplatin-induced cold hypersensitivity is due to remodelling of ion channel expression in nociceptors. EMBO Mol. Med. 2011, 3, 266–278. [Google Scholar] [CrossRef]
  7. Kawashiri, T.; Egashira, N.; Kurobe, K.; Tsutsumi, K.; Yamashita, Y.; Ushio, S.; Yano, T.; Oishi, R. L type ca2+ channel blockers prevent oxaliplatin-induced cold hyperalgesia and trpm8 overexpression in rats. Mol. Pain 2012, 8, 7. [Google Scholar] [CrossRef] [Green Version]
  8. Yoon, S.-Y.; Robinson, C.R.; Zhang, H.; Dougherty, P.M. Spinal astrocyte gap junctions contribute to oxaliplatin-induced mechanical hypersensitivity. J. Pain 2013, 14, 205–214. [Google Scholar] [CrossRef] [Green Version]
  9. Janes, K.; Wahlman, C.; Little, J.W.; Doyle, T.; Tosh, D.K.; Jacobson, K.A.; Salvemini, D. Spinal neuroimmune activation is independent of t-cell infiltration and attenuated by a3 adenosine receptor agonists in a model of oxaliplatin-induced peripheral neuropathy. Brain Behav. Immun. 2015, 44, 91–99. [Google Scholar] [CrossRef] [Green Version]
  10. Xiao, W.H.; Bennett, G.J. Effects of mitochondrial poisons on the neuropathic pain produced by the chemotherapeutic agents, paclitaxel and oxaliplatin. Pain 2012, 153, 704–709. [Google Scholar] [CrossRef] [Green Version]
  11. Riva, B.; Dionisi, M.; Potenzieri, A.; Chiorazzi, A.; Cordero-Sanchez, C.; Rigolio, R.; Carozzi, V.A.; Lim, D.; Cavaletti, G.; Marmiroli, P. Oxaliplatin induces ph acidification in dorsal root ganglia neurons. Sci. Rep. 2018, 8, 1–12. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  12. Krishnan, A.V.; Goldstein, D.; Friedlander, M.; Kiernan, M.C. Oxaliplatin-induced neurotoxicity and the development of neuropathy. Muscle Nerve Off. J. Am. Assoc. Electrodiagn. Med. 2005, 32, 51–60. [Google Scholar] [CrossRef] [PubMed]
  13. Kim, W.; Kim, M.J.; Go, D.; Min, B.-I.; Na, H.S.; Kim, S.K. Combined effects of bee venom acupuncture and morphine on oxaliplatin-induced neuropathic pain in mice. Toxins 2016, 8, 33. [Google Scholar] [CrossRef] [PubMed]
  14. Ling, B.; Authier, N.; Balayssac, D.; Eschalier, A.; Coudore, F. Behavioral and pharmacological description of oxaliplatin-induced painful neuropathy in rat. Pain 2007, 128, 225–234. [Google Scholar] [CrossRef]
  15. Kim, W.; Chung, Y.; Choi, S.; Min, B.-I.; Kim, S.K. Duloxetine protects against oxaliplatin-induced neuropathic pain and spinal neuron hyperexcitability in rodents. Int. J. Mol. Sci. 2017, 18, 2626. [Google Scholar] [CrossRef] [Green Version]
  16. Chae, H.K.; Kim, W.; Kim, S.K. Phytochemicals of cinnamomi cortex: Cinnamic acid, but not cinnamaldehyde, attenuates oxaliplatin-induced cold and mechanical hypersensitivity in rats. Nutrients 2019, 11, 432. [Google Scholar] [CrossRef] [Green Version]
  17. Lee, J.H.; Go, D.; Kim, W.; Lee, G.; Bae, H.; Quan, F.S.; Kim, S.K. Involvement of spinal muscarinic and serotonergic receptors in the anti-allodynic effect of electroacupuncture in rats with oxaliplatin-induced neuropathic pain. Korean J. Physiol. Pharmacol. 2016, 20, 407–414. [Google Scholar] [CrossRef] [Green Version]
  18. Choi, S.; Chae, H.K.; Heo, H.; Hahm, D.-H.; Kim, W.; Kim, S.K. Analgesic effect of melittin on oxaliplatin-induced peripheral neuropathy in rats. Toxins 2019, 11, 396. [Google Scholar] [CrossRef] [Green Version]
  19. Li, D.; Kim, W.; Shin, D.; Jung, Y.; Bae, H.; Kim, S.K. Preventive effects of bee venom derived phospholipase a2 on oxaliplatin-induced neuropathic pain in mice. Toxins 2016, 8, 27. [Google Scholar] [CrossRef] [Green Version]
  20. Zain, M.; Bonin, R.P. Alterations in evoked and spontaneous activity of dorsal horn wide dynamic range neurons in pathological pain: A systematic review and analysis. Pain 2019, 160, 2199–2209. [Google Scholar] [CrossRef]
  21. Jamieson, S.; Liu, J.; Connor, B.; McKeage, M. Oxaliplatin causes selective atrophy of a subpopulation of dorsal root ganglion neurons without inducing cell loss. Cancer Chemother. Pharmacol. 2005, 56, 391–399. [Google Scholar] [CrossRef] [PubMed]
  22. Hains, B.C.; Saab, C.Y.; Waxman, S.G. Changes in electrophysiological properties and sodium channel nav1. 3 expression in thalamic neurons after spinal cord injury. Brain 2005, 128, 2359–2371. [Google Scholar] [CrossRef] [PubMed]
  23. Park, S.; Krishnan, A.; Lin, C.; Goldstein, D.; Friedlander, M.; Kiernan, M. Mechanisms underlying chemotherapy-induced neurotoxicity and the potential for neuroprotective strategies. Curr. Med. Chem. 2008, 15, 3081–3094. [Google Scholar] [CrossRef] [PubMed]
  24. Kagiava, A.; Tsingotjidou, A.; Emmanouilides, C.; Theophilidis, G. The effects of oxaliplatin, an anticancer drug, on potassium channels of the peripheral myelinated nerve fibres of the adult rat. Neurotoxicology 2008, 29, 1100–1106. [Google Scholar] [CrossRef] [PubMed]
  25. Cummins, T.R.; Sheets, P.L.; Waxman, S.G. The roles of sodium channels in nociception: Implications for mechanisms of pain. Pain 2007, 131, 243–257. [Google Scholar] [CrossRef] [Green Version]
  26. Rogers, M.; Tang, L.; Madge, D.J.; Stevens, E.B. The role of sodium channels in neuropathic pain. In Seminars in Cell & Developmental Biology; Elsevier: Amsterdam, The Netherlands, 2006; pp. 571–581. [Google Scholar]
  27. Eckel, F.; Schmelz, R.; Adelsberger, H.; Erdmann, J.; Quasthoff, S.; Lersch, C. Prevention of oxaliplatin-induced neuropathy by carbamazepine. A pilot study. Deutsche Medizinische Wochenschrift 1946 2002, 127, 78–82. [Google Scholar] [CrossRef]
  28. Gamelin, E.; Gamelin, L.; Bossi, L.; Quasthoff, S. Clinical aspects and molecular basis of oxaliplatin neurotoxicity: Current management and development of preventive measures. Semin. Oncol. 2002, 29 (Suppl. 15), 21–33. [Google Scholar] [CrossRef]
  29. Webster, R.G.; Brain, K.L.; Wilson, R.H.; Grem, J.L.; Vincent, A. Oxaliplatin induces hyperexcitability at motor and autonomic neuromuscular junctions through effects on voltage-gated sodium channels. Br. J. Pharmacol. 2005, 146, 1027–1039. [Google Scholar] [CrossRef]
  30. Li, L.; Shao, J.; Wang, J.; Liu, Y.; Zhang, Y.; Zhang, M.; Zhang, J.; Ren, X.; Su, S.; Li, Y. Mir-30b-5p attenuates oxaliplatin-induced peripheral neuropathic pain through the voltage-gated sodium channel nav1. 6 in rats. Neuropharmacology 2019, 153, 111–120. [Google Scholar] [CrossRef]
  31. Adelsberger, H.; Quasthoff, S.; Grosskreutz, J.; Lepier, A.; Eckel, F.; Lersch, C. The chemotherapeutic oxaliplatin alters voltage-gated na+ channel kinetics on rat sensory neurons. Eur. J. Pharmacol. 2000, 406, 25–32. [Google Scholar] [CrossRef]
  32. Sittl, R.; Lampert, A.; Huth, T.; Schuy, E.T.; Link, A.S.; Fleckenstein, J.; Alzheimer, C.; Grafe, P.; Carr, R.W. Anticancer drug oxaliplatin induces acute cooling-aggravated neuropathy via sodium channel subtype nav1. 6-resurgent and persistent current. Proc. Natl. Acad. Sci. USA 2012, 109, 6704–6709. [Google Scholar] [CrossRef] [Green Version]
  33. Grolleau, F.; Gamelin, L.; Boisdron-Celle, M.; Lapied, B.; Pelhate, M.; Gamelin, E. A possible explanation for a neurotoxic effect of the anticancer agent oxaliplatin on neuronal voltage-gated sodium channels. J. Neurophysiol. 2001, 85, 2293–2297. [Google Scholar] [CrossRef] [PubMed]
  34. Benoit, E.; Brienza, S.; Dubois, J. Oxaliplatin, an anticancer agent that affects both Na+ and K+ channels in frog peripheral myelinated axons. Gen. Physiol. Biophys. 2006, 25, 263. [Google Scholar] [PubMed]
  35. Alberti, P.; Canta, A.; Chiorazzi, A.; Fumagalli, G.; Meregalli, C.; Monza, L.; Pozzi, E.; Ballarini, E.; Rodriguez-Menendez, V.; Oggioni, N. Topiramate prevents oxaliplatin-related axonal hyperexcitability and oxaliplatin induced peripheral neurotoxicity. Neuropharmacology 2020, 164, 107905. [Google Scholar] [CrossRef] [PubMed]
  36. Mauldin, S.K.; Gibbons, G.; Wyrick, S.D.; Chaney, S.G. Intracellular biotransformation of platinum compounds with the 1, 2-diaminocyclohexane carrier ligand in the l1210 cell line. Cancer Res. 1988, 48, 5136–5144. [Google Scholar] [PubMed]
  37. Rush, A.M.; Cummins, T.R.; Waxman, S.G. Multiple sodium channels and their roles in electrogenesis within dorsal root ganglion neurons. J. Physiol. 2007, 579, 1–14. [Google Scholar] [CrossRef]
  38. Caldwell, J.H.; Schaller, K.L.; Lasher, R.S.; Peles, E.; Levinson, S.R. Sodium channel nav1. 6 is localized at nodes of ranvier, dendrites, and synapses. Proc. Natl. Acad. Sci. USA 2000, 97, 5616–5620. [Google Scholar] [CrossRef] [Green Version]
  39. Deuis, J.R.; Zimmermann, K.; Romanovsky, A.A.; Possani, L.D.; Cabot, P.J.; Lewis, R.J.; Vetter, I. An animal model of oxaliplatin-induced cold allodynia reveals a crucial role for nav1. 6 in peripheral pain pathways. Pain® 2013, 154, 1749–1757. [Google Scholar] [CrossRef] [Green Version]
  40. Wilson, M.J.; Yoshikami, D.; Azam, L.; Gajewiak, J.; Olivera, B.M.; Bulaj, G.; Zhang, M.-M. Μ-conotoxins that differentially block sodium channels nav1. 1 through 1.8 identify those responsible for action potentials in sciatic nerve. Proc. Natl. Acad. Sci. USA 2011, 108, 10302–10307. [Google Scholar] [CrossRef] [Green Version]
  41. Zedan, A.H.; Hansen, T.F.; Svenningsen, Å.F.; Vilholm, O.J. Oxaliplatin-induced neuropathy in colorectal cancer: Many questions with few answers. Clin. Colorectal Cancer 2014, 13, 73–80. [Google Scholar] [CrossRef] [PubMed]
  42. Heide, R.; Bostock, H.; Ventzel, L.; Grafe, P.; Bergmans, J.; Fuglsang-Frederiksen, A.; Finnerup, N.B.; Tankisi, H. Axonal excitability changes and acute symptoms of oxaliplatin treatment: In vivo evidence for slowed sodium channel inactivation. Clin. Neurophysiol. 2018, 129, 694–706. [Google Scholar] [CrossRef]
  43. Horowitz, S.H. The diagnostic workup of patients with neuropathic pain. Anesthesiol. Clin. 2007, 25, 699–708. [Google Scholar] [CrossRef] [PubMed]
  44. Forstenpointner, J.; Oberlojer, V.C.; Naleschinski, D.; Höper, J.; Helfert, S.M.; Binder, A.; Gierthmühlen, J.; Baron, R. A-fibers mediate cold hyperalgesia in patients with oxaliplatin-induced neuropathy. Pain Pract. 2018, 18, 758–767. [Google Scholar] [CrossRef] [PubMed]
  45. Krishnan, A.V.; Goldstein, D.; Friedlander, M.; Kiernan, M.C. Oxaliplatin and axonal na+ channel function in vivo. Clin. Cancer Res. 2006, 12, 4481–4484. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  46. Hille, B. Charges and potentials at the nerve surface: Divalent ions and ph. J. Gen. Physiol. 1968, 51, 221–236. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  47. Schmitt, L.-I.; Leo, M.; Kleinschnitz, C.; Hagenacker, T. Oxaliplatin modulates the characteristics of voltage-gated calcium channels and action potentials in small dorsal root ganglion neurons of rats. Mol. Neurobiol. 2018, 55, 8842–8855. [Google Scholar] [CrossRef]
  48. Yamamoto, K.; Tsuboi, M.; Kambe, T.; Abe, K.; Nakatani, Y.; Kawakami, K.; Utsunomiya, I.; Taguchi, K. Oxaliplatin administration increases expression of the voltage-dependent calcium channel α2δ-1 subunit in the rat spinal cord. J. Pharmacol. Sci. 2016, 130, 117–122. [Google Scholar] [CrossRef] [Green Version]
  49. Gamelin, L.; Boisdron-Celle, M.; Delva, R.; Guérin-Meyer, V.; Ifrah, N.; Morel, A.; Gamelin, E. Prevention of oxaliplatin-related neurotoxicity by calcium and magnesium infusions: A retrospective study of 161 patients receiving oxaliplatin combined with 5-fluorouracil and leucovorin for advanced colorectal cancer. Clin. Cancer Res. 2004, 10, 4055–4061. [Google Scholar] [CrossRef] [Green Version]
  50. Grothey, A.; Nikcevich, D.A.; Sloan, J.A.; Kugler, J.W.; Silberstein, P.T.; Dentchev, T.; Wender, D.B.; Novotny, P.J.; Chitaley, U.; Alberts, S.R. Intravenous calcium and magnesium for oxaliplatin-induced sensory neurotoxicity in adjuvant colon cancer: Ncctg n04c7. J. Clin. Oncol. 2011, 29, 421. [Google Scholar] [CrossRef]
  51. Bennett, D.L.; Clark, A.J.; Huang, J.; Waxman, S.G.; Dib-Hajj, S.D. The role of voltage-gated sodium channels in pain signaling. Physiol. Rev. 2019, 99, 1079–1151. [Google Scholar] [CrossRef]
  52. Lersch, C.; Schmelz, R.; Eckel, F.; Erdmann, J.; Mayr, M.; Schulte-Frohlinde, E.; Quasthoff, S.; Grosskreutz, J.; Adelsberger, H. Prevention of oxaliplatin-induced peripheral sensory neuropathy by carbamazepine in patients with advanced colorectal cancer. Clin. Colorectal Cancer 2002, 2, 54–58. [Google Scholar] [CrossRef]
  53. Hoffman, K. Potential prevention and treatment of oxaliplatin associated peripheral neuropathy. J. Clin. Oncol. 2004, 22, 8093. [Google Scholar] [CrossRef]
  54. Hershman, D.L.; Lacchetti, C.; Dworkin, R.H.; Smith, E.M.L.; Bleeker, J.; Cavaletti, G.; Chauhan, C.; Gavin, P.; Lavino, A.; Lustberg, M.B.; et al. Prevention and management of chemotherapy-induced peripheral neuropathy in survivors of adult cancers: American society of clinical oncology clinical practice guideline. J. Clin. Oncol. 2014, 32, 1941–1967. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  55. Smith, E.M.L.; Pang, H.; Cirrincione, C.; Fleishman, S.; Paskett, E.D.; Ahles, T.; Bressler, L.R.; Fadul, C.E.; Knox, C.; Le-Lindqwister, N. Effect of duloxetine on pain, function, and quality of life among patients with chemotherapy-induced painful peripheral neuropathy: A randomized clinical trial. Jama 2013, 309, 1359–1367. [Google Scholar] [CrossRef] [PubMed]
  56. Wang, S.-Y.; Calderon, J.; Wang, G.K. Block of neuronal na+ channels by antidepressant duloxetine in a state-dependent manner. Anesthesiol. J. Am. Soc. Anesthesiol. 2010, 113, 655–665. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  57. Rolland, J.-F.; Madge, D.; Ford, J.; Rogers, M. Biophysical characterization of duloxetine activity on voltage-gated sodium channels involved in pain transmission. Biophys. J. 2009, 96, 252a. [Google Scholar] [CrossRef] [Green Version]
Table
Table 1. Summary table of studies on oxaliplatin and voltage-gated sodium channels.
Table 1. Summary table of studies on oxaliplatin and voltage-gated sodium channels.
Published YearAuthorModelsInvestigated NerveOxaliplatin DosesDiscoveries
2000Helmuth Adelsberger
et al. [31]		Rat
(1)
Sural, peroneal and vagal nerve
(2)
DRG
(3)
Hippocampal neurons
250 μM
(1)
Increase CAP amplitude in A- than C-fiber (218 ± 68% vs. 36 ± 29%, n = 7)
(2)
Shift of the voltage–response relationship to negative potentials (10 mV, n = 13/18)
(3)
Reduction of the maximal amplitude (0.65 ± 0.23, n = 13)
(4)
Slowdown of the inactivation kinetics of the channels (2.6 ± 1.45, n = 13)
(5)
No effect on Na+ current in hippocampal neurons (n = 8)
2001Francoise Grolleau
et al. [33]		Cockroach
(Periplaneta americana)		DUM neurons100
And
500 μM
(1)
Effect of extracellular and intracellular application on maximum peak Na+ currents (100 μM, 16.7 ± 5.8% vs. 52.8 ± 3.3%, n = 6 and 5, respectively)
(2)
Maximum blocking effect of Na+ currents (1 mM, 59.2 ± 1.6%, n = 3)
(3)
Dose dependent effect of oxalate (1 mM, 50.5 ± 7.5%, n = 3)
(4)
Reduction of both depolarizing phase and posthyperpolarization associated with increase in AC duration (500 μM)
2005Richard G. Webster
et al. [29]		Mouse
(C57/BL6 and Balb/c)
(1)
Hemidiaphragm (Phrenic nerve, C57/BL6)
(2)
Vas deferens (Balb/c)
500 μM
(1)
Increased MEPP frequency in hemidiaphragm compared to control (0.5 mM, 1.07 ± 0.08 vs. 32.10 ± 7.49, respectively, n = 4)
(2)
Increase MEPP prevented by TTX pretreatment (1 μM)
(3)
Carbamazepine reduced MEPP and EPP amplitudes (0.3 mM, 16.1% and 21.6%, respectively, n = 4)
(4)
β-pompilidotoxin induced multiple EPPs (0.1 mM)
(5)
Frequency of sEJP increased (0.5 mM, 0.11 ± 0.03 s−1 vs. 0.042 ± 0.015 s−1, np = 7)
(6)
TTX administration abolished all sEJPs (100 nM)
(7)
Carbamazepine reduced amplitude of EJPs (0.3 mM, 55 ± 12%, n = 5)
2006Evelyne Benoit
et al. [34]		Frog
(Rana esculenta)		Sciatic nerve (Nodes of Ranvier of single sensory or motor myelinated axon)1,
10
And
100 μM
(1)
Decreased nodal Na+ currents peak after 5–7 min application (10 and 100 μM, 77 ± 8% and 41 ± 7% of its control value, respectively, n = 10–11)
(2)
Shift of Na+ currents peak towards negative membrane potentials (1 and 100 μM, −3.5 ± 0.5 mV and −8.8 ± 0.3 mV compared to control, n = 3–4)
(3)
A negative shift in the steady-state inactivation-voltage curve of Na+ currents peak (1 and 100 μM, 8.5 ± 2.8 mV and 10 ± 2.2 mV more negative compared to control, n = 3–4)
2012Ruth Sittl
et al. [32]		Human (Five male patients 57 ± 9 years of age)
and Scn8amed Mice
(1)
Sural nerves
(CAP recording)
(2)
Saphenous nerve
(single-fiber recordings)
10
And
100 μM
(1)
Cooling-induced CAP after potentials observed in A-, but not C-fibers both in human and mice nerves (100 μM, at ~20 °C, n = 8 and 55 for human and mouse respectively)
(2)
After potentials absent in nerves from Scn8amed/med mice
(3)
Enhancement of Nav1.6-mediated resurgent sodium currents in myelinated DRG neurons (control 22.2 ± 3.4 pA/pF at 30 °C vs. 21.9 ± 4.9 at 22 °C pA/pF, n = 5–8, P = 0.56; oxaliplatin, 29.6 ± 3.8 pA/pF at 30 °C vs. 57.3 ± 10.6 pA/pF at 22 °C, n = 5–11, P < 0.05).
(4)
TTX-sensitive Nav activation and steady-state fast inactivation not affected (n = 4–10)
2013Jennifer R. Deuis
et al. [39]		Mouse
(C57BL/6J)
Nav1.3–/–
Nav1.8–/–
And
Nav1.9–/–		Behavioral measurement on the left hind paw2.5 mM;
40 μg/paw
(1)
Cold allodynia not affected in Nav1.3–/–, Nav1.8–/– and Nav1.9–/– mice (2.5 mM; 103 ± 11%, 98 ± 15% and 107 ± 8% of control, respectively)
(2)
Cold allodynia unchanged by Nav1.7 selective inhibitor ProTxII and 1.8 selective small molecule inhibitor A803467 injection (3 nM and 10 μM; 115 ± 17% and 90 ± 17% of control, respectively)
(3)
Intraplantar injection of TTX inhibited cold allodynia upon exposure to a cooled surface (3 μM; 19 ± 4% of control)
(4)
Intraplantar injection of μ-Conotoxin TIIIA (Nav1.1, 1.2 and 1.4 inhibiter) did not decrease cold allodynia (10 or 100 μM)
(5)
Intraplantar injection of GIIIA (Nav1.1 and Nav1.6 inhibitor) showed complete reversal of cold allodynia (10 μM; 14 ± 9% of control)
(6)
Intraplantar Cn2 (Nav1.6 activator) injection elicited mechanical, but not cold allodynia (1 and 30 nM)
2019Paola Alberti
et al. [35]		Rat
(Wistar)		Caudal Nerve5 mg/kg (i.v.)
(1)
Proximal and distal caudal nerve sensory nerve AC amplitude and distal caudal nerve sensory conduction velocity were significantly reduced compared to control (0.001, < 0.001, 0.04, respectively)
(2)
Increase in refractory period (P = 0.002) and refractoriness at 2 and 2.5 msec (P = 0.049)
2019Lei Li
et al. [30]		Rat (Sprague-Dawley)DRG (L4-6)2.4,
3.2,
4.0 mg/kg (i.p.)
And
0.1 μM
(1)
Intragastric administration of Bulleyaconitine A (a sodium channel inhibitor) significantly attenuated mechanical allodynia (0.1 mg/kg; F(2,66) = 218.92, P < 0.0001)
(2)
The expression levels of Nav1.6 mRNA (F(3,15) = 60.98, P < 0.0001), protein (F(3,15) = 15.00, P = 0.0002) and Nav1.6 (F(3,11) = 18.67, P = 0.0006 and F(3,11) = 52.54, P < 0.0001) in the DRG were significantly increased compared to control (measured by RT-qPCR and immunofluorescent staining)
(3)
Both Scn8a mRNA (F(4,14) = 26.95, P < 0.0001) and Nav1.6 protein (F(4,19) = 57.93, P < 0.0001) level increased, while miR-30b (F(4,14) = 14.60, P = 0.0004) decreased after dose dependent administration of oxaliplatin (0.1, 0.2, and 0.3 μM) into the culture medium
(4)
miR-30b agomir treatment markedly mitigated the oxaliplatin induced increase in Scn8a mRNA and Nav1.6 protein
(5)
Intrathecal administration of the miR-30b agomir inhibited the expression of Nav1.6 in DRG neurons (F (3.11) = 17.87, P = 0.0007)
Abbreviations: AC, Action Potential; CAP, Compound Action Potential; DRG, Dorsal Root Ganglia; DUM, Dorsal Unpaired Median; EJP, Excitatory Junction Potential; MEPP, Miniature Endplate Potential; I.P., Intraperitoneal; I.V., Intravenous; TTX, Tetrodotoxin.

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Kim, W. Effect of Oxaliplatin on Voltage-Gated Sodium Channels in Peripheral Neuropathic Pain. Processes 2020, 8, 680. https://doi.org/10.3390/pr8060680

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Kim W. Effect of Oxaliplatin on Voltage-Gated Sodium Channels in Peripheral Neuropathic Pain. Processes. 2020; 8(6):680. https://doi.org/10.3390/pr8060680

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Kim, Woojin. 2020. "Effect of Oxaliplatin on Voltage-Gated Sodium Channels in Peripheral Neuropathic Pain" Processes 8, no. 6: 680. https://doi.org/10.3390/pr8060680

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Kim, W. (2020). Effect of Oxaliplatin on Voltage-Gated Sodium Channels in Peripheral Neuropathic Pain. Processes, 8(6), 680. https://doi.org/10.3390/pr8060680

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