Protective Effects of p-Coumaric Acid Isolated from Vaccinium bracteatum Thunb. Leaf Extract on Corticosterone-Induced Neurotoxicity in SH-SY5Y Cells and Primary Rat Cortical Neurons
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Preparation and Standardization of NET-D1602
2.3. SH-SY5Y Cell Cultures
2.4. Primary Culture of Rat Cortical Cells
2.5. MTT Assay
2.6. Measurement of SOD and Catalase Activities
2.7. Treatment of Drugs in Cultured Cells for Western Blotting
2.8. Nuclear and Cytoplasmic Fractionation and Whole Cell Lysates of Cultured Cells
2.9. Western Blotting
2.10. Statistical Analysis
3. Results
3.1. Detection of Neuroprotection and HPLC Analysis
3.2. Effects of NET-D1602 and p-Coumaric Acid on CORT-Induced Neurotoxicity in SH-SY5Y Cells and Primary Cultured Rat Cortical Neurons
3.3. Effects of NET-D1602 and p-Coumaric Acid on the Activities of SOD and Catalase in CORT-Treated SH-SY5Y Cells
3.4. Effects of NET-D1602 and p-Coumaric Acid on the ERK/CREB Signaling in Primary Cultured Rat Cortical Neurons
3.5. Effects of Kinase Inhibitors on the CREB Phosphorylation of NET-D1602 and p-Coumaric Acid in Primary Cultured Rat Cortical Neurons
3.6. Effects of NET-D1602 and p-Coumaric Acid on ERK, Akt/mTOR, and CREB Phosphorylation in CORT-Induced Neurotoxicity
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
- Graham, S.H. Introduction to special issue: Neurovascular aging-A driving force for neurological dysfunction in stroke and neurodegenerative diseases. Ageing Res. Rev. 2017, 34, 1–2. [Google Scholar] [CrossRef] [PubMed]
- Prentice, H.; Modi, J.P.; Wu, J.Y. Mechanisms of Neuronal Protection against Excitotoxicity, Endoplasmic Reticulum Stress, and Mitochondrial Dysfunction in Stroke and Neurodegenerative Diseases. Oxid. Med. Cell. Longev. 2015, 2015, 964518. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Doolin, K.; Farrell, C.; Tozzi, L.; Harkin, A.; Frodl, T.; O’Keane, V. Diurnal hypothalamic-pituitary-adrenal axis measures and inflammatory marker correlates in major depressive disorder. Int. J. Mol. Sci. 2017, 18, 2226. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Abraham, I.M.; Meerlo, P.; Luiten, P.G. Concentration dependent actions of glucocorticoids on neuronal viability and survival. Dose Response A Publ. Int. Hormesis Soc. 2006, 4, 38–54. [Google Scholar] [CrossRef] [Green Version]
- Tomita, H.; Ziegler, M.E.; Kim, H.B.; Evans, S.J.; Choudary, P.V.; Li, J.Z.; Meng, F.; Dai, M.; Myers, R.M.; Neal, C.R.; et al. G protein-linked signaling pathways in bipolar and major depressive disorders. Front. Genet. 2013, 4, 297. [Google Scholar] [CrossRef] [Green Version]
- Dworkin, S.; Mantamadiotis, T. Targeting CREB signalling in neurogenesis. Expert Opin. Ther. Targets. 2010, 14, 869–879. [Google Scholar] [CrossRef]
- Ferreira, P.S.; Victorelli, F.D.; Fonseca-Santos, B.; Chorilli, M.A. Review of Analytical Methods for p-Coumaric Acid in Plant-Based Products, Beverages, and Biological Matrices. Crit. Rev. Anal. Chem. 2019, 49, 21–31. [Google Scholar] [CrossRef]
- Vauzour, D.; Corona, G.; Spencer, J.P. Caffeic acid, tyrosol and p-coumaric acid are potent inhibitors of 5-S-cysteinyl-dopamine induced neurotoxicity. Arch. Biochem. Biophys. 2010, 501, 106–111. [Google Scholar] [CrossRef]
- Kim, H.B.; Lee, S.; Hwang, E.S.; Maeng, S.; Park, J.H. p-Coumaric acid enhances long-term potentiation and recovers scopolamine-induced learning and memory impairments. Biochem. Biophy. Res. Commun. 2017, 492, 493–499. [Google Scholar] [CrossRef] [PubMed]
- Wang, L.; Xu, H.N.; Yao, H.; Zhang, H. Phenolic composition and radical scavenging capacity of Vaccinium bracteatum Thunb. Leaves. Int. J. Food Prop. 2011, 14, 721–725. [Google Scholar] [CrossRef]
- Kwon, S.H.; Ma, S.X.; Ko, Y.H.; Seo, J.Y.; Lee, B.R.; Lee, T.H.; Taek, H.L.; Sun, Y.K.; Seok-Yong, L.; Choon-Gon, J. Vaccinium bracteatum Thunb. exerts anti-inflammatory activity by inhibiting NF-κB activation in BV-2 microglial cells. Biomol. Ther. 2016, 24, 543–551. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Zheng, Y.; Chen, L.; Liu, Y.; Shi, L.; Wan, S.; Wang, L. Evaluation of antimicrobial activity of water-soluble flavonoids extract from Vaccinium bracteatum Thunb. leaves. Food Sci. Biotechnol. 2019, 28, 1853–1859. [Google Scholar] [CrossRef] [PubMed]
- Wang, L.; Zhang, X.T.; Yao, H.Y. The protective effect of Vaccinium bracteatum Thunb. leaves and the extract against light injury of retina. J. Xi’an Jiaotong Uni. 2006, 27, 284–287. [Google Scholar]
- Wang, L.; Zhang, Y.; Xu, M.; Wang, Y.; Cheng, S.; Liebrecht, A.; Haifeng, Q.; Hui, Z.; Xiguang, Q. Anti-diabetic activity of Vaccinium bracteatum Thunb. leaves’ polysaccharide in STZ-induced diabetic mice. Int. J. Biol. Macromol. 2013, 61, 317–321. [Google Scholar] [CrossRef]
- Oh, D.R.; Yoo, J.S.; Kim, Y.; Kang, H.; Lee, H.; Lm, S.J.; Choi, E.J.; Jung, M.A.; Bae, D.; Oh, K.N.; et al. Vaccinium bracteatum leaf extract reverses chronic restraint stress-induced depression-like behavior in mice: Regulation of hypothalamic-pituitary-adrenal axis, serotonin turnover systems, and ERK/Akt phosphorylation. Front. Pharmacol. 2018, 9, 604. [Google Scholar] [CrossRef] [Green Version]
- Oh, D.R.; Kim, Y.; Im, S.; Oh, K.N.; Shin, J.; Jeong, C.; Kim, Y.; Choi, E.J.; Choi, C. Vaccinium bracteatum improves spatial learning and memory by regulating N-methyl-D-aspartate receptors and Tau phosphorylation in chronic restraint stress-induced memory impaired mice. Am. J. Chin. Med. 2021, 49, 69–94. [Google Scholar] [CrossRef]
- Kim, Y.; Shin, J.; Oh, D.R.; Kim, D.W.; Lee, H.S.; Choi, C. Complete chloroplast genome sequences of Vaccinium bracteatum Thunb.,V. vitis-idaea L., and V. uliginosum L. (Ericaceae). Mitochondrial DNA Part B. 2020, 5, 1843–1844. [Google Scholar] [CrossRef] [Green Version]
- Kim, S.; Yun, H.M.; Baik, J.H.; Chung, K.C.; Nah, S.Y.; Rhim, H. Functional interaction of neuronal Cav1.3 L-type calcium channel with ryanodine receptor type 2 in the rat hippocampus. J. Biol. Chem. 2007, 282, 32877–32889. [Google Scholar] [CrossRef] [Green Version]
- Odaka, H.; Adachi, N.; Numakawa, T. Impact of glucocorticoid on neurogenesis. Neural Regen. Res. 2017, 12, 1028–1035. [Google Scholar] [CrossRef] [PubMed]
- Lima Giacobbo, B.; Doorduin, J.; Klein, H.C.; Dierckx, R.; Bromberg, E.; de Vries, E.F.J. Brain-derived neurotrophic factor in brain disorders: Focus on neuroinflammation. Mol. Neurobiol. 2019, 56, 3295–3312. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Zhang, J.; Chu, C.J.; Li, X.L.; Yao, S.; Yan, B.; Ren, H.L.; Xu, N.Y.; Liang, Z.T.; Zhao, Z.Z. Isolation and identification of antioxidant compounds in Vaccinium bracteatum Thunb. by UHPLC-Q-TOF LC/MS and their kidney damage protection. J. Funct. Foods. 2014, 11, 62–70. [Google Scholar] [CrossRef]
- Motta, J.; Jung, I.; Azzolin, V.; Teixeira, C.F.; Braun, L.E.; de Oliveira Nerys, D.A.; Motano, M.A.E.; Duarte, M.; Maia-Ribeiro, E.A.; da Cruz, I.D.; et al. Avocado oil (Persea americana) protects SH-SY5Y cells against cytotoxicity triggered by cortisol by the modulation of BDNF, oxidative stress, and apoptosis molecules. J. Food Biochem. 2021, 45. [Google Scholar] [CrossRef]
- Pusceddu, M.M.; Nolan, Y.M.; Green, H.F.; Robertson, R.C.; Stanton, C.; Kelly, P.; Cryan, J.F.; Dinan, T.G. The omega-3 polyunsaturated fatty acid docosahexaenoic acid (DHA) reverses corticosterone-induced changes in cortical neurons. Int. J. Neuropsychopharmacol. 2016, 19. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Lee, S.; Kim, H.B.; Hwang, E.S.; Kim, E.S.; Kim, S.S.; Jeon, T.D.; Song, M.C.; Lee, J.S.; Chung, M.C.; Maeng, S.; et al. Antidepressant-like effects of p-coumaric acid on LPS-induced depressive and inflammatory changes in rats. Exp. Neurobiol. 2018, 27, 189–199. [Google Scholar] [CrossRef]
- Sakamula, R.; Thong-Asa, W. Neuroprotective effect of p-coumaric acid in mice with cerebral ischemia reperfusion injuries. Metab. Brain Dis. 2018, 33, 765–773. [Google Scholar] [CrossRef]
- Guven, M.; Aras, A.B.; Akman, T.; Sen, H.M.; Ozkan, A.; Salis, O.; Sehitoglu, I.; Kalkan, Y.; Silan, C.; Deniz, M.; et al. Neuroprotective effect of p-coumaric acid in rat model of embolic cerebral ischemia. Iran. J. Basic Med. Sci. 2015, 18, 356–363. [Google Scholar] [PubMed]
- Velusamy, T.; Panneerselvam, A.S.; Purushottam, M.; Anusuyadevi, M.; Pal, P.K.; Jain, S.; Essa, M.M.; Guillemin, G.J.; Kandasamy, M. Protective effect of antioxidants on neuronal dysfunction and plasticity in Huntington’s disease. Oxid. Med. Cell. Longev. 2017, 2017, 3279061. [Google Scholar] [CrossRef]
- Blendy, J.A. The role of CREB in depression and antidepressant treatment. Biol. Psychiatry 2006, 59, 1144–1150. [Google Scholar] [CrossRef] [PubMed]
- Notaras, M.; Buuse, M. Neurobiology of BDNF in fear memory, sensitivity to stress, and stress-related disorders. Mol. Psychatry. 2020, 25, 2251–2274. [Google Scholar] [CrossRef]
- Gibon, J.; Deloulme, J.C.; Chevallier, T.; Ladeveze, E.; Abrous, D.N.; Bouron, A. The antidepressant hyperforin increases the phosphorylation of CREB and the expression of TrkB in a tissue-specific manner. Int. J. Neuropsychopharmacol. 2013, 16, 189–198. [Google Scholar] [CrossRef] [Green Version]
- Wang, H.; Xu, J.; Lazarovici, P.; Quirion, R.; Zheng, W. cAMP Response Element-Binding Protein (CREB): A possible signaling molecule link in the pathophysiology of schizophrenia. Front. Mol. Neurosci. 2018, 11, 255. [Google Scholar] [CrossRef]
- Sabitha, R.; Nishi, K.; Gunasekaran, V.P.; Agilan, B.; David, E.; Annamalai, G.; Vinothkumar, R.; Perumal, M.; Subbiah, L.; Ganeshan, M. p-Coumaric acid attenuates alcohol exposed hepatic injury through MAPKs, apoptosis and Nrf2 signaling in experimental models. Chem. Biol. Interact. 2020, 321, 109044. [Google Scholar] [CrossRef]
- Han, X.; Guo, J.; You, Y.; Zhan, J.; Huang, W. p-Coumaric acid prevents obesity via activating thermogenesis in brown adipose tissue mediated by mTORC1-RPS6. FASEB J. Off. Publ. Fed. Am. Soc. Exp. Biol. 2020, 34, 7810–7824. [Google Scholar] [CrossRef] [PubMed]
- Duman, R.S.; Voleti, B. Signaling pathways underlying the pathophysiology and treatment of depression: Novel mechanisms for rapid-acting agents. Trends. Neurosci. 2012, 35, 47–56. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Matsuda, S.; Ikeda, Y.; Murakami, M.; Nakagawa, Y.; Tsuji, A.; Kitagishi, Y. Roles of PI3K/AKT/GSK3 pathway involved in psychiatric illnesses. Diseases 2019, 7, 22. [Google Scholar] [CrossRef] [PubMed] [Green Version]
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. |
© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Share and Cite
Oh, D.-R.; Kim, M.-J.; Choi, E.-J.; Kim, Y.; Lee, H.-S.; Bae, D.; Choi, C. Protective Effects of p-Coumaric Acid Isolated from Vaccinium bracteatum Thunb. Leaf Extract on Corticosterone-Induced Neurotoxicity in SH-SY5Y Cells and Primary Rat Cortical Neurons. Processes 2021, 9, 869. https://doi.org/10.3390/pr9050869
Oh D-R, Kim M-J, Choi E-J, Kim Y, Lee H-S, Bae D, Choi C. Protective Effects of p-Coumaric Acid Isolated from Vaccinium bracteatum Thunb. Leaf Extract on Corticosterone-Induced Neurotoxicity in SH-SY5Y Cells and Primary Rat Cortical Neurons. Processes. 2021; 9(5):869. https://doi.org/10.3390/pr9050869
Chicago/Turabian StyleOh, Dool-Ri, Moon-Jong Kim, Eun-Jin Choi, Yujin Kim, Hak-Sung Lee, Donghyuck Bae, and Chulyung Choi. 2021. "Protective Effects of p-Coumaric Acid Isolated from Vaccinium bracteatum Thunb. Leaf Extract on Corticosterone-Induced Neurotoxicity in SH-SY5Y Cells and Primary Rat Cortical Neurons" Processes 9, no. 5: 869. https://doi.org/10.3390/pr9050869