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Article

The Separation and Purification of Ellagic Acid from Phyllanthus urinaria L. by a Combined Mechanochemical-Macroporous Resin Adsorption Method

Key Laboratory for Green Pharmaceutical Technologies and Related Equipment of the Ministry of Education, College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou 310014, China
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Author to whom correspondence should be addressed.
Separations 2021, 8(10), 186; https://doi.org/10.3390/separations8100186
Submission received: 10 September 2021 / Revised: 2 October 2021 / Accepted: 7 October 2021 / Published: 14 October 2021

Abstract

Ellagic acid is a phenolic compound that exhibits both antimutagenic and anticarcinogenic activity in a wide range of assays in vitro and in vivo. It occurs naturally in some foods such as raspberries, strawberries, grapes, and black currants. In this study, a valid and reliable method based on mechanochemical-assisted extraction (MCAE) and macroporous adsorption resin was developed to extract and prepare ellagic acid from Phyllanthus urinaria L. (PUL). The MCAE parameters, acidolysis, and macroporous adsorption resin conditions were investigated. The key MCAE parameters were optimized as follows: the milling time was 5 min, the ball mill speed was 100 rpm, and the ball mill filling rate was 20.9%. Sulfuric acid with a concentration of 0.552 mol/L was applied for the acidolysis with the optimized acidolysis time of 30 min and acidolysis temperature of 40 °C. Additionally, the XDA-8D macroporous resin was chosen for the purification work. Both the static and dynamic adsorption tests were carried out. Under the optimized conditions, the yield of ellagic acid was 10.2 mg/g, and the content was over 97%. This research provided a rapid and efficient method for the preparation of ellagic acid from the cheaply and easily obtained PUL. Meanwhile, it is relatively low-cost work that can provide a technical basis for the comprehensive utilization of PUL.
Keywords: Phyllanthus urinaria L.; ellagic acid; mechanochemical-assisted extraction; macroporous adsorption resin Phyllanthus urinaria L.; ellagic acid; mechanochemical-assisted extraction; macroporous adsorption resin

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MDPI and ACS Style

Guo, Z.; Xiong, S.; Xie, Y.; Liang, X. The Separation and Purification of Ellagic Acid from Phyllanthus urinaria L. by a Combined Mechanochemical-Macroporous Resin Adsorption Method. Separations 2021, 8, 186. https://doi.org/10.3390/separations8100186

AMA Style

Guo Z, Xiong S, Xie Y, Liang X. The Separation and Purification of Ellagic Acid from Phyllanthus urinaria L. by a Combined Mechanochemical-Macroporous Resin Adsorption Method. Separations. 2021; 8(10):186. https://doi.org/10.3390/separations8100186

Chicago/Turabian Style

Guo, Zili, Shuting Xiong, Yuanyuan Xie, and Xianrui Liang. 2021. "The Separation and Purification of Ellagic Acid from Phyllanthus urinaria L. by a Combined Mechanochemical-Macroporous Resin Adsorption Method" Separations 8, no. 10: 186. https://doi.org/10.3390/separations8100186

APA Style

Guo, Z., Xiong, S., Xie, Y., & Liang, X. (2021). The Separation and Purification of Ellagic Acid from Phyllanthus urinaria L. by a Combined Mechanochemical-Macroporous Resin Adsorption Method. Separations, 8(10), 186. https://doi.org/10.3390/separations8100186

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