Effect of Physical and Enzymatic Pre-Treatment on the Nutritional and Functional Properties of Fermented Beverages Enriched with Cricket Proteins
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Ultrasound Pre-Treatment
2.3. γ-Irradiation Treatment
2.4. Enzymatic Hydrolysis and Combined Treatments
2.5. Beverage Preparation
2.6. Protein Solubility
2.7. Measurement of Sulfhydryl (SH) Bonds
2.8. Surface Hydrophobicity
2.9. Molecular Characterization by Fourier Transform Infrared (FTIR) Spectroscopy
2.10. In Vitro Digestibility
2.11. Peptide Profile (SEC-HPLC)
2.12. Statistical Analysis
3. Results and Discussion
3.1. Protein Solubility
3.2. Sulfhydryl Groups and Surface Hydrophobicity
3.3. ATR-FTIR Analysis
3.4. In Vitro Digestibility
3.5. Molecular Weight (Mw) Distribution by SEC Analysis
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Acknowledgments
Conflicts of Interest
References
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Treatment Effect (Ratio Treatment/Control) | α-Helix 1653 cm−1 | β-Sheets 1623 cm−1 | β-Sheets 1637 cm−1 | Random Coils 1645 cm−1 | β-Turns 1663 cm−1 | β-Turns 1694 cm−1 |
---|---|---|---|---|---|---|
US effect | ||||||
10 min | 1.32 | 1.08 | 0.79 | 1.19 | 1.38 | 1.00 |
20 min | 1.17 | 1.29 | 1.20 | 1.74 | 3.11 | 1.21 |
30 min | 0.88 | 1.18 | 1.01 | 1.83 | 1.55 | 1.40 |
40 min | 0.96 | 1.18 | 1.19 | 1.36 | 1.77 | 1.22 |
IR effect | ||||||
3 kGy | 0.20 | 0.74 | 0.70 | 1.68 | 1.59 | 0.74 |
5 kGy | 0.96 | 0.70 | 0.48 | 1.35 | 1.35 | 0.42 |
7 kGy | 0.18 | 0.67 | 0.55 | 1.66 | 1.08 | 0.50 |
US-IR effect | ||||||
US-IR | 1.02 | 1.07 | 1.00 | 3.47 | 1.70 | 0.55 |
Percentage of MW Distribution, (%) | |||||||||
---|---|---|---|---|---|---|---|---|---|
Samples | MW (Da) | NF 1 (0 min) | NFP 2 (120 min) | NFPT 3 (240 min) | ΔNF7 | F 4 (0 min) | FP 5 (120 min) | FPT 6 (240 min) | ΔF 7 |
Control | >3000 | 6.7 ± 0.3 | 3.9 ± 0.1 | 2.2 ± 0.0 | −67.1 | 6.4 ± 0.0 | 4.6 ± 0.2 | 2.3 ± 0.1 | −62.5 |
3000–260 | 60.0 ± 0.5 | 61.6 ± 0.5 | 63.6 ± 0.8 | 6.0 | 59.1 ± 1.1 | 60.0 ± 0.4 | 63.4 ± 0.6 | 7.6 | |
<260 | 33.3 ± 0.9 | 34.5 ± 0.4 | 34.2 ± 0.7 | 2.7 | 34.5 ± 1.0 | 35.4 ± 0.1 | 34.3 ± 0.6 | −0.6 | |
Cr | >3000 | 5.0 ± 0.61 | 3.8 ± 0.41 | 3.2 ± 0.35 | −36.0 | 4.9 ± 0.7 | 3.8 ± 0.4 | 3.0 ± 0.4 | −38.7 |
3000–260 | 37.7 ± 2.9 | 38.8 ± 0.2 | 43.3 ± 0.1 | 17.9 | 41.4 ± 0.8 | 43.3 ± 0.6 | 45.5 ± 0.5 | 10 | |
<260 | 57.3 ± 3.2 | 57.4 ± 0.5 | 53.5 ± 0.3 | −6.6 | 53.7 ± 0.0 | 52.9 ± 0.2 | 51.5 ± 0.0 | −4.1 | |
US-IR | >3000 | 4.5 ± 0.1 | 3.0 ± 0.1 | 3.3 ± 0.4 | −26.6 | 4.5 ± 0.1 | 3.7 ± 0.2 | 3.5 ± 0.1 | −22.2 |
3000–260 | 37.1 ± 0.7 | 39.3 ± 0.3 | 49.1 ± 0.3 | 32.3 | 44.7 ± 1.61 | 43.1 ± 1.6 | 46.0 ± 0.4 | 2.9 | |
<260 | 58.4 ± 0.6 | 57.7 ± 0.2 | 47.6 ± 0.4 | −18.4 | 50.8 ± 1.4 | 53.2 ± 1.5 | 50.5 ± 0.5 | −0.6 | |
US-E | >3000 | 1.8 ± 0.0 | 1.7 ± 0.1 | 0.9 ± 0.2 | −50.0 | 2.2 ± 0.7 | 1.3 ± 0.0 | 0.9 ± 0.7 | −59.0 |
3000–260 | 38.7 ± 0.3 | 39.4 ± 0.0 | 41.7 ± 0.4 | 7.7 | 39.1 ± 0.4 | 40.5 ± 0.3 | 41.4 ± 1.2 | 5.9 | |
<260 | 59.5 ± 0.3 | 58.9 ± 0.1 | 57.4 ± 0.4 | −3.5 | 58.7 ± 0.3 | 58.2 ± 0.2 | 57.7 ± 0.1 | −1.7 | |
US-EWC | >3000 | 1.3 ± 0.6 | 0.8 ± 0.0 | 0.5 ± 0.0 | −61.5 | 1.8 ± 0.3 | 0.7 ± 0.0 | 0.5 ± 1.0 | −72.2 |
3000–260 | 29.4 ± 0.3 | 30.0 ± 0.0 | 30.5 ± 0.3 | 3.7 | 30.1 ± 0.3 | 31.1 ± 0.0 | 34.7 ± 2.5 | 15.3 | |
<260 | 69.3 ± 0.4 | 69.1 ± 0.5 | 69.0 ± 0.4 | −0.5 | 68.2 ± 0.4 | 68.2 ± 0.2 | 64.8 ± 2.8 | −4.9 | |
US-IRE | >3000 | 1.3 ± 0.2 | 0.8 ± 0.5 | 0.7 ± 0.6 | −85.7 | 1.3 ± 0.1 | 0.7 ± 0.4 | 0.3 ± 1.0 | −76.9 |
3000–260 | 18.9 ± 0.3 | 19.3 ± 0.4 | 18.5 ± 0.1 | −2.1 | 20.4 ± 0.4 | 19.4 ± 0.0 | 19.0 ± 2.5 | −6.8 | |
<260 | 79.8 ± 0.3 | 79.8 ± 1.0 | 80.7 ± 0.7 | 1.1 | 78.3 ± 0.4 | 79.8 ± 0.3 | 80.7 ± 2.8 | 3.1 |
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Dridi, C.; Millette, M.; Aguilar, B.; Manus, J.; Salmieri, S.; Lacroix, M. Effect of Physical and Enzymatic Pre-Treatment on the Nutritional and Functional Properties of Fermented Beverages Enriched with Cricket Proteins. Foods 2021, 10, 2259. https://doi.org/10.3390/foods10102259
Dridi C, Millette M, Aguilar B, Manus J, Salmieri S, Lacroix M. Effect of Physical and Enzymatic Pre-Treatment on the Nutritional and Functional Properties of Fermented Beverages Enriched with Cricket Proteins. Foods. 2021; 10(10):2259. https://doi.org/10.3390/foods10102259
Chicago/Turabian StyleDridi, Chaima, Mathieu Millette, Blanca Aguilar, Johanne Manus, Stephane Salmieri, and Monique Lacroix. 2021. "Effect of Physical and Enzymatic Pre-Treatment on the Nutritional and Functional Properties of Fermented Beverages Enriched with Cricket Proteins" Foods 10, no. 10: 2259. https://doi.org/10.3390/foods10102259