1. Introduction
The meat from slow-growing native chicken breeds is considered an alternative to commercial white broiler meat. In recent years, the consumer demand for the meat of native chickens has been growing immensely. This rising demand is mostly attributed to better taste and flavour, and low-fat content. The meat of the slow-growing native chickens is considered healthy due to their slow growth and environmentally friendly as they do not require feeding of antimicrobial/antibiotic growth promoters, that is a usual practice in the rapidly growing broilers. Furthermore, native chickens are hardy and reported to be resistant to most of the commonly occurring bacterial and parasitic diseases [
1,
2] and therefore they are least likely to be administered with antimicrobial agents for prophylactic and therapeutic purposes.
Kadaknath is the unique and most popular breed among the notified and registered chicken breeds of India. These birds can thrive under low input backyard and free-range farming in tropical conditions that are otherwise harsh to the improved exotic breeds. Kadaknath is a slow-growing bird, and its growth rate is lesser than that reported for Aseel, a famous native chicken breed of India [
3]. The meat of this breed is considered to be a delicacy due to its perceived taste and flavour. Furthermore, the meat of this breed is presumably known to have better nutrient profile as compared to commercial broilers. Therefore, Kadaknath meat is being sold in niche market with a premium price (2 to 3 times) than that of broiler meat. This breed has attracted tremendous attention due to its unique black colour hyper-pigmentation. The hyper-pigmentation is due to the
fibromelanosis (
Fm) phenomenon caused by the excessive deposition of eumelanin in almost all parts of the body including muscles and internal organs. The meat of this breed is also black [
4]. The black pigmentation character may be similar to the one noticed in the other two famous breeds; Silkie breed from China and Ayam Cemani of Indonesia [
5]. Kadaknath is a slow growing native chicken with low to moderate egg production potential [
6].
Meat quality is a function of the genotype, nutrition and rearing practises. Previous studies have reported the differences in carcass and meat quality traits between slow-growing and fast-growing chicken lines [
7,
8]. Further, the genotype of birds (growth rate) is also known to play an important role in the nutrient composition (proximate composition and amino acid profile) of the meat. Previously, it was reported that the meat of slow growing birds (Thai indigenous chickens and meat type birds) had more protein, less fat and ash content as compared to the meat of fast growing commercial broilers and they differed in the amino acid composition as well [
9,
10]. It was also reported that the fat and cholesterol content of slow growing Thai native chicken’s meat was lower as compared to the fast-growing Rhode Island Red chickens [
11]. The differences were also observed in the colour of muscles between slow growing native chicken (Aseel) and broilers [
12,
13]. Recently, it was reported that the genotype of birds not only affected the proximate principles but also the amino acid composition of meat [
14]. The meat of Polverara chickens was reported to contain more protein and less fat and had a better profile of indispensable amino acids as compared to commercial broilers [
14].
The meat of Kadaknath is reported to be a rich source of functional biomolecules such as carnosine [
15]. However, there is a little information about the nutritional value of the Kadaknath meat
vis à vis broiler chicken. It is hypothesized that the meat of this slow-growing Kadaknath differs from that of fast-growing chickens for various carcass and meat quality traits including the nutritional profile. Therefore, the aim of the present study was to characterize the Kadaknath breed for carcass and meat quality traits, including nutritional and amino acid profile in comparison with the broilers.
4. Discussion
The information about the meat quality traits and nutrient composition of Kadaknath meat
vis à vis broiler helps the consumers to make an informed decision in purchasing and consuming the meat of this breed. The growth of the Kadaknath like any other native chicken is slow. They attain the market weight of about 1.5 to 2.0 kg (live bodyweight) only by 24–28 weeks of age as compared to 5 to 6 weeks required for broilers to reach this weight. In this study, it was seen that even with similar bodyweight as that of broilers, Kadaknath had significantly lesser dressed weight and hence significantly lesser dressing and breast yield. The lesser yield observed in the Kadaknath breed might be due to less muscle mass and higher percentage of non-edible portions such as head, shank, and blood and feathers as a proportion of live bodyweight of this breed. Further, high muscle and bone mass of broilers might have contributed to the higher dressing yield in broilers. The dressing percentage of Kadaknath was similar to that of other native chickens such as Aseel Peela (69.54, 70.8%) [
4,
12], and Ghagus (70.2%) [
19]. However, the dressing percentage recorded in this study is higher than those observed in other studies for Kadaknath (65.2%) birds slaughtered at a younger age of 20 weeks [
20] and Nicobari (65.2%) breed (Haunshi et al., Unpublished).
Breast yield was higher in broilers while the yield of legs, wings, back and neck were higher in Kadaknath. This difference was due to the selection of broilers for higher breast yield. As native birds are required to run faster and fly to escape from predators in the free-range rearing systems, they are naturally selected for stronger legs, wings, neck and back muscles leading to higher proportion in legs, wings, and back (
Figure 1). Weights of leg and breast parts of Kadaknath were comparable to those of other native chickens such as Aseel (22.7 and 17.1%), Ghagus (23.4 and 16.3%) and Nicobari (20.51 and 15.91%) breeds [
12,
20], (Haunshi et al., unpublished). Higher yield of liver, heart and gizzard (giblets) observed in broilers is due to higher feed intake, assimilation of nutrients and metabolism that are required for rapid growth in such a short period of time (35–42 days). Native birds are slow in growth and their feed intake is also very low hence have a lesser percentage of internal organs related to digestion and metabolism as compared to the fast-growing broilers. The giblet percentage observed for broilers in the present study was comparable with those reported for white broilers [
13].
The abdominal fat of Kadaknath was also negligible while broilers had more abdominal and subcutaneous fat (
Figure S1). Like any other native chickens, Kadaknath birds had less abdominal fat as observed in slow-growing native breeds such as Aseel Peela (0.02%) chicken [
4], but it was lower than that reported for Aseel (0.75%) [
12] and Nicobari (1.5%) breed. The abdominal fat observed in broilers is similar to the one (1.85%) reported previously for broilers [
12]. Significantly higher meat–bone ratio observed in broilers can be explained by the fact that broilers were selected for a high growth rate and hence resulted in higher meat yield. Drum part of Kadaknath has significantly more meat and hence had higher MBR as compared to that of broiler. This finding is desirable and economically significant as Indian consumers prefer the drum part of the chicken leg as compared to the breast meat of the chicken.
Although the weight of the dressed weight and cut up parts was higher in male birds there was no difference in most of the carcass traits including dressing percentage between male and female birds of Kadaknath. These findings were almost similar to the ones observed in Vanashree (evolved from native chicken) chicken [
21]. However, the effect of sex on meat quality traits in this breed needs to be investigated.
The colour of the meat plays an important role in consumers’ preference for the meat. Generally, the meat of the Kadaknath is black in colour as compared to any other chickens. Instrumental measurements also showed that both breast and thigh meats of Kadaknath were significantly darker and had more yellowness than broilers. The colour of the chicken meat is influenced by various factors such as muscle type, genotype, age, sex, diet, amount of myoglobin and heme pigments in the muscles, processing, etc. [
22]. The meat of the slow-growing chickens is reported to be darker and show more yellow colour as compared to the fast-growing chickens [
9,
11,
12,
13]. The lightness, redness and yellowness colour values of breast muscles of fast-growing broilers were reported to be in the range of 53.59 to 55.54, 3.05 to −0.93, 4.97 to 8.48, respectively [
7,
23,
24]. The colour values of breast muscles of broilers observed in our study were almost within this range. The colour values of the Kadaknath breed were comparable to those reported (39.32, 2.49 and 4.02) for Thai indigenous chickens [
9], but lesser than that reported for (53.92, 2.30 and 7.66) Aseel breed of chicken [
13]. In Kadaknath birds, the excess deposition of melanin pigment in muscles contributed to the dark colour of the meat. Furthermore, the black colour pigmentation appears to reduce the chroma of the Kadaknath meat. To our knowledge, this is the first study to objectively measure the colour of the Kadaknath meat in comparison with the broilers.
The pH of the meat plays a vital role in meat quality. The low ultimate pH may lead to acid meat with undesirable characteristics of pale soft exudate meat as it is negatively correlated with drip loss, cooking loss and texture [
25]. The less decline in pH of Kadaknath meat is in a desirable direction and indicates better meat quality. It was reported that pH and colour of muscles are highly correlated. As muscles with lower pH tend to be lighter in colour while muscles with higher pH tend to be darker in colour [
7]. Like the present study, Debut [
8] observed significant negative correlation of pH24h with lightness and yellowness. Further, Le Bihan-Duval [
25] reported negative correlation (genetic) between pH with lightness and yellowness colours of muscles. Colour variables (
L*,
a* and
b*) are related to each other. As observed in the present study, other studies also reported a positive correlation between lightness with yellowness and chroma while a negative correlation between lightness and redness in breast meat of male broilers [
8,
26]. It was well documented that breast and thigh muscles differ in colour characteristics as breast muscles tend to be lighter, yellower and less red as compared to the leg meats as seen in the Kadaknath breed. In this study, it was observed that breast meat of both Kadaknath and broilers was lighter and less red as compared to their respective thigh meats. Chen et al. [
27] observed that irrespective of breeds, the breast meat was lighter and less red as compared to their respective thigh meats.
The Kadaknath meat had a favourable nutrient composition as it had relatively high protein content, less fat, less moisture and less ash content compared to the meat of broilers. The significant difference observed between these two types of birds in the nutrient composition may be attributed to the genetic makeup (breed) and the age difference the birds. It was reported that as the age of the bird increases, the composition of body tissues changes and that the protein content increases and moisture content decrease [
28]. There was a considerable variation in the age of the Kadaknath and broilers because of their different market age. Furthermore, selection for rapid growth in broilers resulted in excess fat deposition in muscles as it was observed for the abdominal fat. The protein content of Kadaknath was higher while that of fat and ash was lesser than those reported for the Aseel breed with more or less similar moisture content [
29]. Similar to our findings, Thai native chickens were reported to have higher protein, lesser fat and lesser ash as compared to broilers [
9]. Likewise, Fanatico [
10] reported that the breast muscles of slow-growing birds had more protein and less fat compared to fast-growing ones. The breast muscles of Polverara, a slow-growing native chicken breed of Italy had higher protein and less fat and moisture content as compared to commercial hybrid birds [
14]. It appears that growth rate of birds besides genotype, greatly influences the nutrient composition of the chicken meat.
Considerable difference was seen in the amino acid content of breast muscles of Kadaknath and broilers. Out of 19 amino acids investigated, the Kadaknath was significantly higher in the content of 11 amino acids, while the broiler was significantly higher in 3 amino acids content. Similar to the proximate composition, variation seen in the amino acid composition between two types of bids might be due to either the genetic makeup or the age of the birds. The higher content of methionine and lysine in broilers might be due to the supplementation of synthetic amino acids such as lysine and methionine to support the rapid growth of broiler diets. while the male breeder diet fed to Kadaknath had no additional supplementation of these synthetic amino acids.
When the WHO/FAO/UNA recommendations [
30] for the daily requirements of indispensable amino acids for a 60 kg adult were taken into consideration, the consumption 100 g meat of Kadaknath or broilers met more than 100% of the daily requirement of lysine, methionine, phenyl alanine, valine, methionine + cysteine, phenyl alanine + tyrosine, and threonine (97% in Kadaknath) amino acids. However, the daily requirement of leucine (49 vs. 40%), histidine (5 vs. 6%) and tryptophan (2 vs. 1%) amino acids was partially met by Kadaknath and broiler meats. Broiler meat had slight edge over Kadaknath meat in meeting the requirement of threonine amino acid while Kadaknath had edge over broiler for leucine amino acid. However, both breeds met almost same level of requirements of histidine and tryptophan amino acids. Therefore, Kadaknath and broilers did not differ considerably in meeting the daily requirements of indispensable amino acids.
Amino acids which are known to provide sweet and umami (savoury) taste attributes to the meat are alanine, asparagine, glutamic acid, glycine, serine and threonine [
31,
32]. Kadaknath breast meat was significantly higher in these tasty amino acids (with the exception of threonine) as compared to the broilers. Similarly, the meat of the Thai indigenous chickens was reported to have higher glutamic acid content than broiler muscles [
8]. Likewise, the Korean native chickens considered to be superior in flavour had higher content of tasty amino acids than broilers [
33]. These findings explain the preference or likeness of people for the meat of native chickens including the Kadaknath breed over that of broilers. There is a perception among the consumers that native chicken meat has better taste and flavour as compared to broiler meat and that difference in amino acid content might be contributing the perception of consumers.