Enhanced Stability and Oral Bioavailability of Cannabidiol in Zein and Whey Protein Composite Nanoparticles by a Modified Anti-Solvent Approach
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Fabrication of CBD-Loaded Composite Nanoparticles
2.3. Particle Size and Zeta-Potential
2.4. High Performance Liquid Chromatography (HPLC)
2.5. Encapsulation Efficiency (EE) and Loading Capacity (LC)
2.6. Water Solubility
2.7. Re-Dispersibility of Freeze-Dried Nanoparticles
2.8. X-ray Diffraction (XRD)
2.9. Fourier Transform Infrared Spectrometry (FT-IR)
2.10. Transmission Electron Microscopy (TEM)
2.11. Stability
2.12. In Vivo Bioavailability Study
2.13. Statistical Analysis
3. Results and Discussion
3.1. Particle Size and Zeta-Potential
3.2. Encapsulation Efficiency (EE), Loading Capacity (LC) and Water Solubility
3.3. Re-Dispersibility of Freeze-Dried Composite Nanoparticles
3.4. XRD Diffractogram
3.5. FT-IR Spectra
3.6. Microstructure Observed by TEM
3.7. Physicochemical Stability
3.8. Storage Stability
3.9. Bioavailability Analysis of CBD
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Wang, C.; Wang, J.; Sun, Y.; Freeman, K.; Mchenry, M.A.; Wang, C.; Guo, M. Enhanced Stability and Oral Bioavailability of Cannabidiol in Zein and Whey Protein Composite Nanoparticles by a Modified Anti-Solvent Approach. Foods 2022, 11, 376. https://doi.org/10.3390/foods11030376
Wang C, Wang J, Sun Y, Freeman K, Mchenry MA, Wang C, Guo M. Enhanced Stability and Oral Bioavailability of Cannabidiol in Zein and Whey Protein Composite Nanoparticles by a Modified Anti-Solvent Approach. Foods. 2022; 11(3):376. https://doi.org/10.3390/foods11030376
Chicago/Turabian StyleWang, Ce, Jia Wang, Yonghai Sun, Kalev Freeman, Monique Alyssa Mchenry, Cuina Wang, and Mingruo Guo. 2022. "Enhanced Stability and Oral Bioavailability of Cannabidiol in Zein and Whey Protein Composite Nanoparticles by a Modified Anti-Solvent Approach" Foods 11, no. 3: 376. https://doi.org/10.3390/foods11030376
APA StyleWang, C., Wang, J., Sun, Y., Freeman, K., Mchenry, M. A., Wang, C., & Guo, M. (2022). Enhanced Stability and Oral Bioavailability of Cannabidiol in Zein and Whey Protein Composite Nanoparticles by a Modified Anti-Solvent Approach. Foods, 11(3), 376. https://doi.org/10.3390/foods11030376