Ultrasound Improved the Non-Covalent Interaction of β-Lactoglobulin with Luteolin: Regulating Human Intestinal Microbiota and Conformational Epitopes Reduced Allergy Risks
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Sample Preparation
2.3. Far-UV Circular Dichroism Spectra
2.4. Fourier Transform Infrared Measurement
2.5. Intrinsic Fluorescence Intensity and UV Spectrometry Measurement
2.6. Surface Hydrophobicity Determination
2.7. Molecular Docking
2.8. Determination of IgG/IgE Binding Ability
2.9. Degranulation of Human Basophil (KU812) Cells
2.10. Determination of Human Intestinal Microbiota
2.11. High-Throughput Sequencing and Microbial Analysis
2.12. Statistical Analysis
3. Results and Discussion
3.1. Infrared Spectral Analysis
3.2. Circular Dichroism Analysis
3.3. Analysis of Intrinsic Fluorescence and UV Absorption Spectra
3.4. Surface Hydrophobicity Analysis
3.5. Molecular Docking
3.6. IgG/IgE Binding Ability and Degranulation Analysis of KU812 Cells
3.7. Human Intestinal Microbiota Analysis
3.8. Ultrasound Improved the Non-Covalent Interaction of β-Lactoglobulin with Luteolin: Effect on the Structure, Allergenicity and Human Intestinal Microbiota
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Sample | α-Helix (%) | β-Sheet (%) | β-Turn (%) | Random (%) |
---|---|---|---|---|
N-LG | 13.24 ± 0.38 b | 29.14 ± 0.74 c | 22.96 ± 0.17 a | 34.64 ± 0.13 a |
U-LG | 14.54 ± 0.20 a | 33.13 ± 0.31 b | 21.95 ± 0.23 a,b | 30.26 ± 0.36 c |
S-LG–LUT | 2.39 ± 0.23 c | 42.37 ± 0.48 a | 22.33 ± 0.38 a,b | 32.90 ± 0.52 b |
U-LG–LUT | 0.61 ± 0.12 d | 43.66 ± 0.76 a | 21.77 ± 0.71 b | 33.84 ± 0.81 a,b |
Samples | Shannon | Simpson | Chao | Coverages |
---|---|---|---|---|
Control | 3.21 ± 0.12 a | 0.09 ± 0.01 c | 252.03 ± 10.98 a | 0.99 ± 0.00 a |
N-LG | 1.68 ± 0.17 c | 0.45 ± 0.06 a | 227.84 ± 13.17 b | 0.99 ± 0.00 a |
U-LG | 1.96 ± 0.33 b,c | 0.38 ± 0.09 a,b | 252.26 ± 9.04 a | 0.99 ± 0.00 a |
S-LG–LUT | 2.39 ± 0.02 b | 0.29 ± 0.01 b | 251.65 ± 3.20 a | 0.99 ± 0.00 a |
U-LG–LUT | 2.32 ± 0.18 b | 0.29 ± 0.06 b | 237.41 ± 2.30 a,b | 0.99 ± 0.00 a |
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Wang, T.; Chen, W.; Shao, Y.; Liu, J.; Tu, Z. Ultrasound Improved the Non-Covalent Interaction of β-Lactoglobulin with Luteolin: Regulating Human Intestinal Microbiota and Conformational Epitopes Reduced Allergy Risks. Foods 2022, 11, 988. https://doi.org/10.3390/foods11070988
Wang T, Chen W, Shao Y, Liu J, Tu Z. Ultrasound Improved the Non-Covalent Interaction of β-Lactoglobulin with Luteolin: Regulating Human Intestinal Microbiota and Conformational Epitopes Reduced Allergy Risks. Foods. 2022; 11(7):988. https://doi.org/10.3390/foods11070988
Chicago/Turabian StyleWang, Titi, Wenmei Chen, Yanhong Shao, Jun Liu, and Zongcai Tu. 2022. "Ultrasound Improved the Non-Covalent Interaction of β-Lactoglobulin with Luteolin: Regulating Human Intestinal Microbiota and Conformational Epitopes Reduced Allergy Risks" Foods 11, no. 7: 988. https://doi.org/10.3390/foods11070988