Beneficial Effects of Three Dietary Cyclodextrins on Preventing Fat Accumulation and Remodeling Gut Microbiota in Mice Fed a High-Fat Diet
Round 1
Reviewer 1 Report
The manuscript requires some editing. Suggestions are below:
- Title is vague- it sounds like the CD had beneficial effects on fat accumulation and not in its prevention- pls reword
- Intro line 48- unclear
- Procedure 2.2
: More info on the experimental period- how long were the animals fed with the diet? and why?
how was feces collected to ensure it e was carried out under sterile conditions
: why 45 % fat- some literature suggest 60 %
: why fibre was used explain more about the fibre, type etc
: using the notation N- denotes that it is a normal diet- strongly suggest to change it to HFD
baseline data?
4. Results: is 9 weeks of supplementation sufficient?- 12 weeks recommended, pls discuss
- Notations a,b,c on all graphs is not explained Also mention sig difference on the graphs
- lines 205-207- rephrase not clear
- lines 220-222- rephrase not clear
- lines 247-250: this is not good enough to establish hepatotoxicity- mention as limitation of study
- line 263- RER in full
- Fig 5d-f what is HEAT, explain
- Interchange of use of fiber and cellulose- be consistent
- limes 311-312 and Fig 7 legend and graph- ensure correct abbreviation of the genes
- Include a section in Discussion/ Conclusion , on the limitations and future work of this/from this study
Author Response
Please see the attachment.
Author Response File: Author Response.docx
Reviewer 2 Report
This is an interesting project and a well-prepared report.
Abstract Lines 19-21: Please include data on alpha-CD responses.
Abstract: Please note in Abstract if the responses to the three cyclodextrins are different, otherwise the last sentence is not logical.
Lines 74-78: Please include a hypothesis for this study.
What do these doses of cyclodextrins represent in a human dose using the Reagan-Shaw equations? Is this a realistic dose in humans?
Line 175: 20% is not a factor.
Author Response
Please see the attachment.
Author Response File: Author Response.docx