Production, Processing, and Protection of Microalgal n-3 PUFA-Rich Oil
Abstract
:1. Introduction
2. Literature Search and Analysis
3. Characteristics of Microalgal Polyunsaturated Fatty Acids
3.1. Microalgal Production of n-3 PUFA
3.1.1. Production of n-3 PUFA in Wild-Type Microalgae
3.1.2. Production of PUFA in Recombinant Microalgae
3.2. Synthetic Pathways of PUFA in Microalgae
4. Commercial Production of PUFA Using Microalgae
4.1. Environmental Factors Influencing Microalgal PUFA Production
4.1.1. Light
4.1.2. Temperature
4.1.3. Salinity
4.1.4. Carbon
4.1.5. Nitrogen
4.1.6. Phosphorus
4.1.7. Other Minerals
4.2. Commercial Cultivation Systems for Microalgal PUFA Production
4.2.1. Photoautotrophic Cultivation
4.2.2. Heterotrophic Fermentation
4.3. Harvesting and Drying of Microalgae
4.4. Pretreatment of Microalgae by Cell Wall Disruption
4.5. Extraction of Microalgae Oil
4.6. Concentration and Purification of Microalgae Oil
5. Protection of PUFA via Microencapsulation
5.1. Spray Drying
5.2. Spray Cooling/Chilling (or Prilling)
5.3. Freeze Drying
5.4. Complex Coacervation
5.5. Nanoemulsions and Self-Emulsifying Emulsions
5.6. Liposome
6. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Species | 14:0 | 16:0 | 16:1 | 18:0 | 18:1 | 18:2 | 18:3 | 20:5 EPA | 22:6 DHA | Total (%DM) | Ref. |
---|---|---|---|---|---|---|---|---|---|---|---|
% of Total Fatty Acids | |||||||||||
Aurantiochytrium | 2.9 | 39.8 | 0.5 | 0.5 | 0.1 | 0.4 | 0.5 | 46.7 | 40–55 | [52,53] | |
Chlamydomonas reinhardtii | 4–20 | 3.8 | 1–16 | 1–10 | 2–22 | 0–5.4 | 12–64 | [54] | |||
Crypthecodiniumcohnii | 18 | 12–45 | 3 | 8 | 13–55 | 25–63 | [53,55,56] | ||||
Dunaliella sp. | 10–28 | 12–16 | 8–11 | 5.9 | 12–36 | 14–21 | 12–46 | [36,53] | |||
Emiliana huxleyi | 18.9 | 10.3 | 10.8 | 19.8 | 9.2 | [41] | |||||
Euglena gracilis | 0.9 | 11.3 | 1.3 | 3.1 | 3.5 | 19.3 | 9.0 | [41] | |||
Heterococcus chodati | 10.0 | 30.6 | 8.1 | 32.6 | [41] | ||||||
Nannochloropsis oculata | 4.2 | 14–24 | 24–30 | 3–5 | 2.9 | 0–9 | 27–49 | 22–37 | [36,37] | ||
Pavlova lutheri | 9.7 | 20.1 | 26.3 | 1.7 | 0.5 | 0.4 | 18.2 | 9.8 | 35 | [41] | |
Phaeodactylum tricornutum | 4.4 | 14–16 | 40–60 | 8.1 | 1.0 | 20–30 | 18.4 | 1.4 | 32 | [57] | |
Scenedesmus obliquus | 30.7 | 23.3 | 6–25 | 8–18 | 10–33 | 21–58 | [58] | ||||
Schizochytrium | 2–8 | 20–45 | 4.8 | 38.4 | 7.9 | 1.2 | 5–12 | 5–50 | 51–71 | [53,59,60] | |
Thraustochytrium sp. | 1.6 | 16.8 | 0.2 | 0.2 | 0.2 | 7.5 | 69 | 13 | [52] | ||
Tribonema vulgare | 4.1 | 13.3 | 34.4 | 10.5 | 17.4 | [41] | |||||
Ulkenia sp. | 25–30 | 10–12 | 5–15 | 15–30 | 20–52 | [52] |
Method | Description | Advantage | Disadvantage | Example | Ref. |
---|---|---|---|---|---|
Sedimentation | Natural gravity sedimentation relies on the particle size of microalgae cells and the density difference of culture environment to harvest; suitable for large biomass and fast sedimentation rate. | Simple; Inexpensive | Affected by cell morphology, not applicable to small-diameter and low-density algae | The filamentous Spirulina platensis having a sedimentation velocity of 0.64 m/h. | [201] |
The diatom Amphora having a velocity of 2.91 m/h. | [202] | ||||
Monoraphidium sp. can be harvested after 24 h with a yield of 98%. | [203] | ||||
Coagulation- Flocculation | Coagulation and flocculation employ chemical (coagulant, zeta potential and pH) or physicochemical (e.g., hydrodynamics) principles to promote cell aggregation and form large particles for separation purposes | Efficient; Inexpensive | Possible coagulant contamination | At high pH, Fe3+, Ca2+ and Mg2+ induced coagulation of C. reinhardtii at <5 mM with >90% biomass harvesting efficiency. | [204] |
Adjusted pH to 9.5 induced coagulation of Chaetoceros calcitrans with 89% of cells were harvested. | [205] | ||||
Centrifugation | Centrifugal method uses acceleration to harvest cells. Various types of centrifugal equipment can be used to harvest microalgae, such as spiral plate centrifuge, decanter centrifuge, disk stack centrifuge, and hydrocyclone. | Efficient; No chemical pollution | High energy consumption; Expensive; Affected by algae morphology | A low biomass harvest efficiency of approx. 50% at 9000× g for Helical A. platensis filaments. | [206] |
A harvest efficiency of 99.3% achieved at 3000× g for 10 min for S. obliquus cells. | [207] | ||||
Flotation | Flotation is a method to transfer microalgae to the surface of culture medium by introducing bubbles (air or ozone), and then collect microalgae by skimming. | Efficient | High energy consumption | Using 3.8 L flotation cell and dissolved air flotation, the harvest efficiency reaches 91%. | [208] |
The heat-induced flotation of Scenedesmus dimorphus at 85 °C, with a harvest efficiency around 80%. | [209] | ||||
Membrane filtration | Membrane filtration can be employed as dead-end or tangential flow filtration mode with membrane pore size varied from 0.1 μm to 10 μm for microfiltration and a few nanometers to 0.1 μm for ultrafiltration membrane respectively. | Pollution-free | Easy to be corroded by medium; Blockages need to be cleaned | Driven by gravity, A. platensis cultures collected using 5 μm nylon membrane with over 90% harvest rate. | [206] |
In the harvesting of Arthrospira sp. with ceramic microfiltration and ultrafiltration membranes, fluxes of 230 L m−2 h−1 and 93 L m−2 h−1 reported, respectively. | [210] | ||||
Drying | The water content of microalgae can be reduced to 10%. There are many drying methods, such as sun-drying, freeze-drying, oven-drying, spray drying, and drum drying. | Lower moisture content; Efficient | Long time; High energy consumption; Uneconomical (except sun drying) | Sun drying is done under sunlight, usually at 18–27 °C; the efficiency is 400–1200 mmol m−2 s−1; takes 2–3 days. | [211] |
Oven drying is done using hot air, usually at 60 °C, takes 12 h. | [211] |
Method | Description | Advantage | Disadvantage | Ref. |
---|---|---|---|---|
Chemical Method | ||||
Hydrothermal | Hydrothermal pretreatment is based on cell wall rupture due to internal pressure build-up from the heating, and hydrolysis of cell wall components by steam explosion, autoclave and water bath treatment. | Unrestricted moisture content; Suitable for low value targets; No chemical reagent; Simple operation | High temperature may oxidize and degrade lipids and other bioactives; High energy consumption | [224,225] |
Acid/Alkaline treatment | Inorganic acid or alkaline solution is used to catalyze and promote hydrolysis processes as an improved version of hydrothermal pretreatment | Efficient; Simple operation | Enhance the soluble chemical oxygen demand; Degradation of sensitive compounds | [226,227] |
Oxidative pretreatment | Strong oxidant (such as ozone or hydrogen peroxide)is used togenerate hydroxyl radicals (OH-) that attack and disrupt the cell walls of microalgae. | Efficient; Suitable for the preparation of biofuels | Destroy highly oxidizable compounds | [228,229,230] |
Physical method | ||||
Pressing | A mechanical force is used to demolish the thick membrane of microalgae and release the oil content. Screw press, extruder, and biomass spraying are the main means of the mechanical pressing. | High purity of the target products; No chemical pollution | Require highdryness of the biomass | [231] |
Bead beating | The membrane of microalgae is disrupted by the action of fast-moving spinning beads. | Simple equipment; Efficient; Wide application range | Need cooling equipment; high temperature destabilize target compounds; Emulsification of products | [232] |
High-pressure homogenization (HPH) | HPH is typically used for emulsification but is also suitable for a large-scale disruption of microalgae cells. | Efficient; High biomass concentration; Reduction of viscosity | Product emulsification affects subsequent extraction | [233,234,235] |
Ultrasonication | Highpressure bubbles and their cavitation generate shock waves, producing high shear forces. | Simple; Suitable for combination with other methods | Oxidation target product; Affect fatty acid chain length; Low efficiency | [236,237] |
Pulsed Electric Field | An intense electric field for very short durations (pulses)applied to microalgae cells to induces reversible or irreversible pores creation (electroporation) on the cell membranes to aid their disruption. | Suitable for freshwater microalgae; Gentle | Low efficiency; Additional steps to remove salt (cost up); Not applicable to marine microalgae | [238,239,240] |
Other novel pretreatment methods | ||||
Enzymatic methods | It is a specific pretreatment method, and requires high selectivity of suitable enzymeson the cell wall structure and composition of a special typeof algae. | High specificity; Mild reaction conditions; Low energy consumption | High enzyme cost; Short process time | [232,241,242] |
CO2 explosion | It pressurizes CO2 inside the cell and increases intracellular gas concentration, leading to excessive expansion and cell rupture. Other non-reactive gasses such as N2 are also used. | Prevent degradation of target products; Efficient | High-cost | [243,244] |
Electricity-based methods | High voltage electric discharges (HVED) utilizes electrodes of needle-plate geometry to deliver high voltage pulses to microalgae suspensions. HVED additionally induces thermal and mechanical effects to the cells due to cavitation and shockwave formation. Non-thermal plasma is another electricity-based method where a needle-to-plate electrode geometry is placed in an argon filled reactor. | No chemical pollution; High extraction rate | Not suitable for extraction of unsaturated fatty acids | [233,245,246] |
Osmotic shock | A week-long pretreatment in which microalgae cells are broken up due to the density difference between cytoplasm and high salt solution. | Simple; High extraction rate | Time consuming | [247,248] |
Ionic liquids | Ionic liquids form a large number of hydrogen bonds that interact with polymers such as cellulose, and destroy the original hydrogen bonds in cellulose and break the cell wall. | High extraction rate; Room temperature | Loss of ions over time; Potential ions pollution | [249,250] |
Viral cell lysis | Virus-assisted cell disruption is a novel method that appeals for low energy consumption. | No chemical pollution | Unknown control factors | [251,252] |
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Ren, X.; Liu, Y.; Fan, C.; Hong, H.; Wu, W.; Zhang, W.; Wang, Y. Production, Processing, and Protection of Microalgal n-3 PUFA-Rich Oil. Foods 2022, 11, 1215. https://doi.org/10.3390/foods11091215
Ren X, Liu Y, Fan C, Hong H, Wu W, Zhang W, Wang Y. Production, Processing, and Protection of Microalgal n-3 PUFA-Rich Oil. Foods. 2022; 11(9):1215. https://doi.org/10.3390/foods11091215
Chicago/Turabian StyleRen, Xiang, Yanjun Liu, Chao Fan, Hao Hong, Wenzhong Wu, Wei Zhang, and Yanwen Wang. 2022. "Production, Processing, and Protection of Microalgal n-3 PUFA-Rich Oil" Foods 11, no. 9: 1215. https://doi.org/10.3390/foods11091215
APA StyleRen, X., Liu, Y., Fan, C., Hong, H., Wu, W., Zhang, W., & Wang, Y. (2022). Production, Processing, and Protection of Microalgal n-3 PUFA-Rich Oil. Foods, 11(9), 1215. https://doi.org/10.3390/foods11091215