Individual and Combined Cytotoxic Effects of Co-Occurring Fumonisin Family Mycotoxins on Porcine Intestinal Epithelial Cell
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals
2.2. Cell Culture and Treatments
2.3. Cell Viability Assay
2.4. Statistical Analysis
3. Results
3.1. Effect of Fumonisins on Cell Viability in IPEC Cells
3.2. Combined Toxicity of Fumonisins in IPEC Cells
3.3. The Mitigation of FB-Induced Cytotoxicity by Natural Substances in IPEC Cells
4. Discussion
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
Abbreviations
FBs | Fumonisin Bs |
FB1 | Fumonisin B1 |
FB2 | Fumonisin B2 |
FB3 | Fumonisin B3 |
CCK-8 | Cell Counting Kit-8 |
DRI | Dose reduction index |
GES-1 | Human gastric epithelial cell line |
IPEC | Porcine intestinal epithelial cells |
ISP-1 | Myriocin |
ID | Inhibitory does |
RVT | Resveratrol |
References
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Dm (μM) | M | r | |
---|---|---|---|
FB1 | 11.3967 | −1.0286 | 0.9968 |
FB2 | 28.9756 | −0.4604 | 0.9699 |
FB3 | 37.2595 | −1.1314 | 0.9955 |
FB1-FB2 | 8.0753 | −0.4398 | 0.9719 |
FB1-FB3 | 11.5549 | −0.6251 | 0.9795 |
FB2-FB3 | 24.1793 | −0.5507 | 0.9859 |
FB1-FB2-FB3 | 9.3569 | −0.7275 | 0.9756 |
Fumonisin | Joint Ratio | ID10 | ID25 | ID50 | ID75 | ID90 |
---|---|---|---|---|---|---|
Combination Index | ||||||
FB1-FB2 | 5:3 | 0.08 | 0.21 | 0.76 | 3.02 | 12.43 |
FB1-FB3 | 5:2 | 0.33 | 0.61 | 1.14 | 2.13 | 3.99 |
FB2-FB3 | 3:2 | 0.59 | 0.52 | 0.68 | 1.37 | 3.47 |
FB1-FB2-FB3 | 5:3:2 | 0.70 | 0.72 | 0.98 | 1.47 | 2.92 |
Fumonisin | Joint Ratio | ID10 | ID25 | ID50 | ID75 | ID90 |
---|---|---|---|---|---|---|
Dose Reduction Index | ||||||
FB1 | 5:3 | 24.64 | 5.89 | 1.41 | 0.34 | 0.08 |
FB2 | 25.55 | 22.85 | 20.42 | 18.26 | 16.33 | |
FB1 | 5:2 | 3.39 | 1.83 | 0.99 | 0.53 | 0.29 |
FB3 | 33.61 | 16.46 | 8.06 | 3.95 | 1.93 | |
FB2 | 3:2 | 8.95 | 6.05 | 4.09 | 2.77 | 1.87 |
FB3 | 17.92 | 6.44 | 2.31 | 0.83 | 0.30 | |
FB1 | 5:3:2 | 2.95 | 1.89 | 1.22 | 0.78 | 0.37 |
FB2 | 84.41 | 42.33 | 17.63 | 7.34 | 3.06 | |
FB3 | 29.26 | 17.07 | 9.96 | 5.81 | 2.35 |
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Yu, S.; Zou, L.; Zhao, J.; Zhu, Y. Individual and Combined Cytotoxic Effects of Co-Occurring Fumonisin Family Mycotoxins on Porcine Intestinal Epithelial Cell. Foods 2023, 12, 2555. https://doi.org/10.3390/foods12132555
Yu S, Zou L, Zhao J, Zhu Y. Individual and Combined Cytotoxic Effects of Co-Occurring Fumonisin Family Mycotoxins on Porcine Intestinal Epithelial Cell. Foods. 2023; 12(13):2555. https://doi.org/10.3390/foods12132555
Chicago/Turabian StyleYu, Song, Lianpeng Zou, Jiawei Zhao, and Yiping Zhu. 2023. "Individual and Combined Cytotoxic Effects of Co-Occurring Fumonisin Family Mycotoxins on Porcine Intestinal Epithelial Cell" Foods 12, no. 13: 2555. https://doi.org/10.3390/foods12132555