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Article

Comparison of Solid-Phase Extraction Sorbents for Monitoring the In Vivo Intestinal Survival and Digestion of Kappa-Casein-Derived Caseinomacropeptide

1
Department of Food Science & Technology, Oregon State University, Corvallis, OR 97331, USA
2
Nutrition Program, School of Biological and Population Health Sciences, College of Public Health and Human Sciences, Oregon State University, Corvallis, OR 97331, USA
*
Author to whom correspondence should be addressed.
Foods 2023, 12(2), 299; https://doi.org/10.3390/foods12020299
Submission received: 6 December 2022 / Revised: 31 December 2022 / Accepted: 5 January 2023 / Published: 8 January 2023
(This article belongs to the Section Food Engineering and Technology)

Abstract

Kappa-casein-derived caseinomacropeptide (CMP)—a 64-amino-acid peptide—is released from kappa-casein after rennet treatment and is one of the major peptides in whey protein isolate (WPI). CMP has anti-inflammatory and antibacterial activities. It also has two major amino acid sequences with different modifications, including glycosylation, phosphorylation, and oxidation. To understand the potential biological role of CMP within the human body, there is a need to examine the extent to which CMP and CMP-derived fragments survive across the digestive tract, where they can exert these functions. In this study, three solid-phase extraction (SPE) methods—porous graphitized carbon (PGC), hydrophilic interaction liquid chromatography (HILIC), and C18 chromatography—were evaluated to determine which SPE sorbent is the most efficient to extract intact CMP and CMP-derived peptides from WPI and intestinal digestive samples prior to LC-MS/MS acquisition. The C18 SPE sorbent was the most efficient in extracting intact CMP and CMP-derived peptides from WPI, whereas the PGC SPE sorbent was the most efficient in extracting CMP-derived peptides from intestinal digesta samples.
Keywords: Kappa-casein-derived caseinomacropeptide; solid-phase extraction; LC-MS/MS; jejunal fluid; whey protein isolate; C18; PGC; HILIC and solid-phase extraction Kappa-casein-derived caseinomacropeptide; solid-phase extraction; LC-MS/MS; jejunal fluid; whey protein isolate; C18; PGC; HILIC and solid-phase extraction

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MDPI and ACS Style

Qu, Y.; Kim, B.-J.; Koh, J.; Dallas, D.C. Comparison of Solid-Phase Extraction Sorbents for Monitoring the In Vivo Intestinal Survival and Digestion of Kappa-Casein-Derived Caseinomacropeptide. Foods 2023, 12, 299. https://doi.org/10.3390/foods12020299

AMA Style

Qu Y, Kim B-J, Koh J, Dallas DC. Comparison of Solid-Phase Extraction Sorbents for Monitoring the In Vivo Intestinal Survival and Digestion of Kappa-Casein-Derived Caseinomacropeptide. Foods. 2023; 12(2):299. https://doi.org/10.3390/foods12020299

Chicago/Turabian Style

Qu, Yunyao, Bum-Jin Kim, Jeewon Koh, and David C. Dallas. 2023. "Comparison of Solid-Phase Extraction Sorbents for Monitoring the In Vivo Intestinal Survival and Digestion of Kappa-Casein-Derived Caseinomacropeptide" Foods 12, no. 2: 299. https://doi.org/10.3390/foods12020299

APA Style

Qu, Y., Kim, B.-J., Koh, J., & Dallas, D. C. (2023). Comparison of Solid-Phase Extraction Sorbents for Monitoring the In Vivo Intestinal Survival and Digestion of Kappa-Casein-Derived Caseinomacropeptide. Foods, 12(2), 299. https://doi.org/10.3390/foods12020299

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