A New Regulatory Network Controls Chilling Injury in Peach Fruit by γ-Aminobutyric Acid
Round 1
Reviewer 1 Report
Dear Authors
In general, the experiments were well performed and the manuscript is well written providing new and interesting information. Thus, the manuscript could be suitable by taking into account the following comments:
Consider modifying the title to better reflect the contents of the paper. You should include the scientific names and cultivar.
Keywords should not include words in the title of the article please revise.
The commercial maturity of peach fruit should be incorporated carefully.
Under which conditions fruits were transferred to lab.
Why did you choose 5 mmol L-1 GABA for the treatment and didn't use other concentration?
Kind regards
Author Response
Dear Reviewer：
Thank you very much for taking the valuable time to review the manuscript and giving valuable comments to improve the quality of the manuscript. These comments were very helpful and the revisions made as a result of them have improved greatly the manuscript. We have endeavored to address all of these comments.
Please find enclosed revised manuscript and a list of the reviewer’s points together with our revisions (with highlight in red color) or responses. We thank you so much for your time and supporting our efforts in the improvement of the manuscript.
Yours sincerely,
Chunbo Song, Zhejiang Wanli University, Ningbo 315100
The People’s Republic of China
Email: [email protected]
Author Response File: 
Author Response.doc
Reviewer 2 Report
It is very well established that exogenous GABA application induces cold tolerance in fruits due to the regulation of polyamines and proline levels and maintains the postharvest quality traits. Therefore, I suggest authors should highlight in the introduction section how this work contributes to filling the knowledge gap in this area. In my opinion, the novelty of this work lies in the evaluation of the regulation of these pathways at the molecular level by the regulation of key gene’s transcript levels. Also indicate what is known about the regulation of the transcription of these genes by the effect of treatments aimed to reduce chilling injury in peach or other fruits.
Please revise the English redaction of the abstract.
The title could be restructured to indicate the study's molecular approach, since my first impression when reading the title was that the study findings may have been already described.
M&M
2.3. Chilling injury symptoms include flesh browning, L* does not reflect the tonality, therefore, a better strategy may be to evaluate de total change of color with a formula that combines L*, and the a* and b* coordinates. At least monitoring changes in the hue angle may bring interesting information on flesh tonality during storage.
2.9. Include at least which genes are going to be evaluated in this study. All response variables have to be well-defined and described.
2.10. Indicate how correlations were performed in this section.
I would prefer the term “unpaired t-test” as indicated in 2.10 instead of “unmatched”
The results of the study seem sound and the conclusions are well supported by the findings.
Author Response
Dear Reviewer：
Thank you very much for taking the valuable time to review the manuscript and giving valuable comments to improve the quality of the manuscript. These comments were very helpful and the revisions made as a result of them have improved greatly the manuscript. We have endeavored to address all of these comments.
Please find enclosed revised manuscript and a list of the reviewer’s points together with our revisions (with highlight in red color) or responses. We thank you so much for your time and supporting our efforts in the improvement of the manuscript.
Yours sincerely,
Chunbo Song, Zhejiang Wanli University, Ningbo 315100
The People’s Republic of China
Email: [email protected]
Author Response File: Author Response.doc
