Hydrogen Sulfide Improves Postharvest Quality of Okra (Abelmoschus esculentus (L.) Moench) Pods by Enhancing Antioxidant Capacity and Delaying Lignification
Abstract
:1. Introduction
2. Materials and Methods
2.1. Experimental Design
2.2. Determination of Weight Loss Rate and Hardness
2.3. Determination of Ascorbic Acid, Soluble Protein, Protopectin, and Soluble Pectin Contents
2.4. Determination of Lignin and Cellulose Contents
2.5. Determination of Malondialdehyde (MDA), Superoxide Anion (O2·−), and Hydrogen Peroxide (H2O2) Contents
2.6. Determination of Catalase (CAT), Ascorbic Acid Peroxidase (APX), and Superoxide Dismutase (SOD) Activities
2.7. Determination of Phenylalanine Ammonialyase (PAL), Cinnamyl Alcohol Dehydrogenase (CAD), 4-Coumarate/CoA Ligase (4CL), Cinnamate 4-Hydroxylase (C4H), and Peroxidase (POD) Activities
2.8. RNA Extraction and Sequencing
2.9. Analysis of Sequencing Data
2.10. Statistical Analysis
3. Results
3.1. Effects of H2S on the Appearance of Okra Pods
3.2. Effects of H2S on Weight Loss Rate and Hardness of Okra Pods
3.3. Effects of H2S on the Contents of Ascorbic Acid, Soluble Protein, Protopectin, and Soluble Pectin in Okra Pods
3.4. Effects of H2S on the Contents of Lignin and Cellulose Content in Okra Pods
3.5. Effects of H2S on the Contents of H2O2, O2·−, and MDA in Okra Pods
3.6. Effects of H2S on the Activities of CAT, APX, SOD in Okra Pods
3.7. Effects of H2S on the Activities of PAL, CAD, 4CL, C4H, and POD in Okra Pods
3.8. Transcriptome Profile of Postharvest Okra Pods RNA Libraries
3.9. Functional Enrichment of DEGs
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Luo, W.; Chen, T.; Gong, X.; Chen, J.; Zhou, W.; Li, J. Hydrogen Sulfide Improves Postharvest Quality of Okra (Abelmoschus esculentus (L.) Moench) Pods by Enhancing Antioxidant Capacity and Delaying Lignification. Foods 2024, 13, 2617. https://doi.org/10.3390/foods13162617
Luo W, Chen T, Gong X, Chen J, Zhou W, Li J. Hydrogen Sulfide Improves Postharvest Quality of Okra (Abelmoschus esculentus (L.) Moench) Pods by Enhancing Antioxidant Capacity and Delaying Lignification. Foods. 2024; 13(16):2617. https://doi.org/10.3390/foods13162617
Chicago/Turabian StyleLuo, Weihua, Tinghui Chen, Xiao Gong, Jingjing Chen, Wei Zhou, and Jihua Li. 2024. "Hydrogen Sulfide Improves Postharvest Quality of Okra (Abelmoschus esculentus (L.) Moench) Pods by Enhancing Antioxidant Capacity and Delaying Lignification" Foods 13, no. 16: 2617. https://doi.org/10.3390/foods13162617