Vasorelaxant Effect and Blood Pressure Reduction Potential of Pitaya Juice Concentrate (Stenocereus huastecorum) Associated with Calcium Channel Blockade
Abstract
:1. Introduction
2. Materials and Methods
2.1. Reagents
2.2. Isolation of PJC
2.3. Guide for Use and Care of the Experimental Model
2.4. Ex Vivo: Measurement of the Vasoactive Response
2.4.1. Experimental Protocol
2.4.2. Isolation of Smooth Muscle Cells from the Thoracic Aorta and Mesenteric Artery for Patch-Clamp Recording
2.4.3. Electrophysiological Recordings
2.5. In Vivo Studies: Measurement of the Vasoactive Response
2.5.1. Experimental Protocols
2.5.2. PJC Effect in SHR and WKY Rats
2.6. Phytochemical Analysis
2.6.1. PJC Fractionation
2.6.2. Bioactive Compounds Profiling by High-Performance Liquid Chromatography Equipped with a Diode Array Detector (HPLC-PDA)
2.6.3. Characterization by UV-Vis Spectroscopy
2.6.4. NMR Analysis of PJC and F1
2.7. Statistical Analyses
3. Results
3.1. PJC-Induced Relaxation of Isolated Thoracic Aortic Rings
3.2. Role of the Endothelium in the Vascular Response to PJC
3.3. Role of K+ Channel in the Vascular Response to PJC
3.4. Role of Cav Channels in the Vascular Response to PJC
3.5. PJC Fractions Relaxed Isolated Thoracic Aorta and Mesenteric Artery Rings
3.6. Cav Channels in the Vascular Response To F1 from PJC
3.7. PJC Induces Antihypertensive Effects in SHRs
3.8. PJC and F1 Profiling
3.9. Characterization of PJC and F1 by UV-Vis Spectroscopy
3.10. NMR Analysis of F1
4. Discussion
5. Conclusions
6. Study Limitations
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
References
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Ramírez-Rodríguez, Y.; Espinosa-Tanguma, R.; Valle-Aguilera, J.R.; Rodríguez-Menchaca, A.A.; Saderi, N.; Salgado-Delgado, R.; Bautista, E.; Garcés, L.; Ramírez, V.; Robledo-Márquez, K.; et al. Vasorelaxant Effect and Blood Pressure Reduction Potential of Pitaya Juice Concentrate (Stenocereus huastecorum) Associated with Calcium Channel Blockade. Foods 2024, 13, 2631. https://doi.org/10.3390/foods13162631
Ramírez-Rodríguez Y, Espinosa-Tanguma R, Valle-Aguilera JR, Rodríguez-Menchaca AA, Saderi N, Salgado-Delgado R, Bautista E, Garcés L, Ramírez V, Robledo-Márquez K, et al. Vasorelaxant Effect and Blood Pressure Reduction Potential of Pitaya Juice Concentrate (Stenocereus huastecorum) Associated with Calcium Channel Blockade. Foods. 2024; 13(16):2631. https://doi.org/10.3390/foods13162631
Chicago/Turabian StyleRamírez-Rodríguez, Yadira, Ricardo Espinosa-Tanguma, Juan Roberto Valle-Aguilera, Aldo A. Rodríguez-Menchaca, Nadia Saderi, Roberto Salgado-Delgado, Elihú Bautista, Luis Garcés, Victoria Ramírez, Karina Robledo-Márquez, and et al. 2024. "Vasorelaxant Effect and Blood Pressure Reduction Potential of Pitaya Juice Concentrate (Stenocereus huastecorum) Associated with Calcium Channel Blockade" Foods 13, no. 16: 2631. https://doi.org/10.3390/foods13162631