Purification and Identification of Novel Dipeptidyl Peptidase IV Inhibitory Peptides Derived from Bighead Carp (Hypophthalmichthys nobilis)
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Bighead Carp Skin Collagen Peptides’ Preparation
2.2.1. Protein Recovery Rate
2.2.2. Preparation of Collagen
2.2.3. SDS-PAGE
2.3. Hydrolysis of Collagen and Measurement of Molecular Weight Distribution
2.3.1. Hydrolysis of Collagen
2.3.2. Measurement of the Molecular Weight Distribution
2.4. Isolation and Purification of Collagen Peptides
2.4.1. Ultrafiltration of Hydrolysates
2.4.2. RP-HPLC Purification
2.5. Determination of DPP-IV Inhibitory Activity
2.6. Identification of Peptide Sequences by Nano-HPLC-MS/MS
2.7. Molecular Docking
2.8. Synthesis of Peptides
2.9. Determination of DPP-IV Inhibition Pattern
2.10. Prediction of Toxicity and Digestibility
2.11. Statistical Analysis
3. Results and Discussion
3.1. SDS-PAGE, Recovery Rate, and Molecular Weight Distribution of Prepared Bighead Carp Skin Collagen
3.2. DPP-IV Inhibitory Activity of Fractions after Purification
3.2.1. RP-HPLC of DPP-IV Inhibitory Peptides
3.2.2. DPP-IV Inhibitory Activity of Fractions
3.3. Identification and Analysis of Peptide Sequences
3.3.1. Nano-HPLC-MS/MS Spectra of Collagen Peptides
3.3.2. Molecular Docking of Collagen Peptides
3.4. DPP-IV Inhibitory Activity of Synthetic Peptides
3.5. DPP-IV Inhibitory Pattern
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Peptide | Docking Score | Hydrophobicity (kcal/mol) | Binding Atoms | Distance (Å) | Interaction |
---|---|---|---|---|---|
IPI (control) | −105.877 | +5.80 | TYR48 ASP545 GLN527 LYS544 | 2.75 2.31 2.23 2.50 | Hydrogen bond |
N/A | N/A | alkyl bonds | |||
GMTGP | −146.637 | +9.92 | ASP545 LYS544 | 2.93 2.10 | Hydrogen bond |
TRP629 TRP629 HIS740 | 4.60 5.26 5.27 | alkyl bonds | |||
GPPGLG | −131.363 | +10.38 | LEU561 TYR48 | 2.83 2.08 | Hydrogen bond |
LEU561 HIS748 TYR752 | 4.63 4.62 5.27 | alkyl bonds | |||
PGLVG | −153.308 | +8.63 | GLU205 GLU205 GLU205 GLU206 ASN710 TYR547 ARG125 | 2.88 2.90 3.07 1.98 2.20 2.73 2.19 | Hydrogen bond |
PHE357 TYR666 TYR662 TYR547 | 4.07 5.18 4.90 4.01 | alkyl bonds | |||
PGF | −149.957 | +7.48 | GLU206 GLU205 | 1.66 2.56 | Hydrogen bond |
VAL656 PHE357 | 5.22 4.68 | alkyl bonds | |||
PGPP | −138.128 | +9.47 | SER209 ARG125 ASN710 | 1.90 2.76 2.75 | Hydrogen bond |
TYR666 TYR666 VAL711 VAL656 TYR662 | 4.91 5.37 5.23 4.34 4.26 | alkyl bonds | |||
PGSP | −137.463 | +9.79 | SER209 GLU205 | 1.28 2.05 | Hydrogen bond |
VAL656 TYR666 TYR662 | 4.88 4.91 4.38 | alkyl bonds | |||
VYP | −143.971 | +6.87 | TYR662 HIS740 SER630 | 6.77 4.72 4.25 | Hydrogen bond |
VAL656 TYR547 | 7.25 6.10 | alkyl bonds | |||
FVA | −154.863 | +6.23 | ASN710 ASN710 GLU206 GLU205 SER209 ARG125 | 4.79 5.27 5.21 4.82 3.99 4.84 | Hydrogen bond |
TYR666 PHE357 VAL656 | 5.21 5.89 6.96 | alkyl bonds | |||
PPGF | −154.322 | +7.62 | ARG125 ARG125 | 5.75 5.91 | Hydrogen bond |
VAL711 TYR666 TYR662 | 6.24 5.88 5.58 | alkyl bonds |
Peptide Sequences | Allergen FP | ToxinPred | Peptide Cutter | Molecular Weight (Da) | IC50 (nM) |
---|---|---|---|---|---|
VYP | Probable allergen | Non-toxin | 2 | 377.19 | 398.87 ± 47.43 a |
FVA | Probable non-allergen | Non-toxin | 1 | 335.18 | 402.02 ± 5.28 a |
PPGF | Probable non-allergen | Non-toxin | 4 | 416.21 | 4.63 ± 1.56 b |
PGLVG | Probable non-allergen | Non-toxin | 3 | 441.26 | 110.20 ± 43.06 c |
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Zheng, H.; Zhao, L.; Xie, Y.; Tan, Y. Purification and Identification of Novel Dipeptidyl Peptidase IV Inhibitory Peptides Derived from Bighead Carp (Hypophthalmichthys nobilis). Foods 2024, 13, 2644. https://doi.org/10.3390/foods13172644
Zheng H, Zhao L, Xie Y, Tan Y. Purification and Identification of Novel Dipeptidyl Peptidase IV Inhibitory Peptides Derived from Bighead Carp (Hypophthalmichthys nobilis). Foods. 2024; 13(17):2644. https://doi.org/10.3390/foods13172644
Chicago/Turabian StyleZheng, Hanzhi, Leyan Zhao, Yushuo Xie, and Yuqing Tan. 2024. "Purification and Identification of Novel Dipeptidyl Peptidase IV Inhibitory Peptides Derived from Bighead Carp (Hypophthalmichthys nobilis)" Foods 13, no. 17: 2644. https://doi.org/10.3390/foods13172644