Psidium guajava Seed Oil Reduces the Severity of Colitis Induced by Dextran Sulfate Sodium by Modulating the Intestinal Microbiota and Restoring the Intestinal Barrier
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials and Reagents
2.2. Experimental Methods
2.2.1. Recording of Body Weight, Disease Activity Index, and Organ Indexes
2.2.2. Morphologic Observation of Colon Tissue
2.2.3. Measurement of Serum Biochemical Indicator
2.2.4. Determination of mRNA Expression Levels of Related Factors in Colonic Tissues
2.2.5. Determination of Short-Chain Fatty Acids in the Cecum
2.2.6. 16S rRNA Sequencing of the Mouse Gut Microbiome
2.2.7. Statistical Analysis
3. Results and Discussion
3.1. Effect of Psidium guajava Seed Oil on Body Weight and DAI Scores of Colitis Mice
3.2. Effect of Psidium guajava Seed Oil on Colonic Organ Index in Colitis Mice
3.3. Effect of Psidium guajava Seed Oil on the Morphology of Colon Tissue in Mice with Colitis
3.4. Effects of Psidium guajava Seed Oil on the Colonic Tissue Structure of Mice with Colitis
3.5. Effects of Psidium guajava Seed Oil on the Ultrastructure of Colon in Colitis Mice
3.6. Effect of Psidium guajava Seed Oil on Inflammatory Factors in Serum and Colonic Tissues of Mice
3.7. Effect of Psidium guajava Seed Oil on the Expression Level of Colon Related mRNA Genes
3.8. Effect of Guarana Acid Oil on the Composition of Intestinal Microbiota of Mice with Colitis
3.8.1. Visualization of TKSO Distribution in Fecal Samples of Colitis Mice
3.8.2. Effect of TKSO on Diversity in Colony Mice
3.8.3. Composition and Abundance Analysis at the Gate Level
3.8.4. Composition and Abundance Analysis at the Genus Level
3.8.5. Correlation Analysis of Short-Chain Fatty Acids, Colon Biochemical Indicators and Intestinal Microbiota
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Composition | C16:0 | C18:0 | C18:1 | C18:2 | C18:3 | C22:2 |
---|---|---|---|---|---|---|
Relative content (%) | 6.016 | 3.291 | 22.448 | 33.177 | 31.761 | 0.559 |
Retain time (min) | 16.514 | 20.64 | 21.568 | 23.107 | 30.497 | 30.846 |
Gene | Primer Sequence | |
---|---|---|
Forward Primer | Reverse Primer | |
IL-4 | CATGGCGTCCCTTCTCCTGTG | GTTGTCATCCTGCTCTTCTTTCTCG |
IL-6 | GTGGTATCCTCTGTGAAGTCTCCTC | TTCTTGGGACTGATGCTGGTGAC |
TNF-α | GTGGTTTGTGAGTGTGAGGGTCTG | CGCTCTTCTGTCTACTGAACTTCG |
Occludin | CCTCTGACCTTGAGTGTGGATGAC | CCTCTGACCTTGAGTGTGGATGAC |
Claudin-1 | GTGTCCTACTTTCCTGCTCCTGTC | AGAAGGTGTTGGCTTGGGATAAGG |
β-actin | TTGTAGAAGGTGTGGTGCCAGATC | GATGGTGGGAATGGGTCAGAAGG |
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Zhang, H.; Shen, G.; Lu, H.; Jiang, C.; Hu, W.; Jiang, Q.; Xiang, X.; Wang, Z.; Chen, L. Psidium guajava Seed Oil Reduces the Severity of Colitis Induced by Dextran Sulfate Sodium by Modulating the Intestinal Microbiota and Restoring the Intestinal Barrier. Foods 2024, 13, 2668. https://doi.org/10.3390/foods13172668
Zhang H, Shen G, Lu H, Jiang C, Hu W, Jiang Q, Xiang X, Wang Z, Chen L. Psidium guajava Seed Oil Reduces the Severity of Colitis Induced by Dextran Sulfate Sodium by Modulating the Intestinal Microbiota and Restoring the Intestinal Barrier. Foods. 2024; 13(17):2668. https://doi.org/10.3390/foods13172668
Chicago/Turabian StyleZhang, Hanwen, Guoxin Shen, Hongling Lu, Chenkai Jiang, Wenjun Hu, Qihong Jiang, Xingwei Xiang, Zongxing Wang, and Lin Chen. 2024. "Psidium guajava Seed Oil Reduces the Severity of Colitis Induced by Dextran Sulfate Sodium by Modulating the Intestinal Microbiota and Restoring the Intestinal Barrier" Foods 13, no. 17: 2668. https://doi.org/10.3390/foods13172668