Isolation, Identification and Antibacterial Characteristics of Lacticaseibacillus rhamnosus YT
Abstract
:1. Introduction
2. Materials and Methods
2.1. Strains and Growth Conditions
2.2. Measurement of Antibacterial Activity
2.3. Screening of LAB with Antibacterial Activity
2.4. Molecular Identification
2.5. Growth and Antibacterial Curve of the Supernatant
2.6. Viable Count Method
2.7. Co-Incubation with Indicator Bacteria Assay
2.8. Sensitivity of Antimicrobial Activity
2.9. Statistical Analysis
3. Results and Discussion
3.1. Screening and Identification of LAB Strain with Antibacterial Ability
3.2. Antibacterial Characteristics of the CFS from L. rhamnosus YT (CFS-YT)
3.2.1. Growth Kinetics and Antimicrobial Activity of the CFS-YT
3.2.2. The Characteristics of the Antimicrobial Activity of CFS-YT
3.3. Antibacterial Characteristics of the L. rhamnosus YT Cells (Cs-YT)
3.3.1. Antibacterial Activity of Cs-YT
3.3.2. Mode of Action
3.3.3. Factors Affecting the Activity of Cs-YT
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Strain | Inhibitory Zone against Indicator Strains (mm) | |||||
---|---|---|---|---|---|---|
B. subtilis | S. aureus | B. cereus | S. enterica | E. coli | P. brenneri | |
2 | ++ | ++ | + | + | ++ | ++ |
4 | ++ | + | - | +++ | +++ | +++ |
6 | + | ++ | - | + | + | +++ |
7 | ++ | +++ | +++ | +++ | +++ | +++ |
9 | + | ++ | + | + | +++ | +++ |
10 | + | + | + | + | ++ | ++ |
11 | ++ | +++ | + | + | +++ | +++ |
18 | - | - | - | - | - | - |
21 | ++ | ++ | +++ | ++ | ++ | ++ |
25 | + | ++ | + | + | ++ | ++ |
27 | + | + | + | + | ++ | +++ |
31 | + | + | + | ++ | ++ | ++ |
32 | - | - | - | - | - | - |
33 | + | + | - | + | ++ | ++ |
34 | - | - | - | - | - | - |
35 | + | + | + | + | ++ | ++ |
44 | ++ | ++ | ++ | + | ++ | ++ |
46 | + | ++ | + | + | ++ | ++ |
53 | - | - | - | - | - | - |
55 | ++++ | ++++ | +++ | +++ | ++++ | ++++ |
57 | - | ++ | + | + | +++ | ++ |
58 | ++++ | ++++ | ++++ | ++++ | ++++ | ++++ |
61 | - | - | - | - | - | + |
65 | + | + | - | - | ++ | +++ |
66 | + | ++ | + | + | ++ | ++ |
69 | ++ | + | ++ | ++ | ++ | +++ |
71 | + | + | + | + | +++ | ++ |
74 | ++ | + | +++ | + | +++ | ++ |
79 | ++ | ++ | ++ | - | +++ | +++ |
82 | - | ++ | + | + | +++ | ++++ |
85 | + | ++ | ++ | + | ++ | +++ |
87 | ++ | ++ | + | - | +++ | +++ |
88 | + | - | + | + | ++ | ++ |
89 | + | + | + | + | ++ | +++ |
96 | + | +++ | ++ | + | ++ | ++++ |
97 | ++ | ++ | ++ | + | +++ | +++ |
98 | - | - | - | - | - | - |
99 | - | + | + | ++ | ++ | +++ |
103 | + | + | + | + | + | + |
105 | + | + | + | ++ | ++ | +++ |
106 | ++ | ++ | ++ | + | +++ | +++ |
111 | ++ | ++ | ++ | + | ++ | ++ |
113 | +++ | ++ | ++ | + | ++++ | ++++ |
116 | ++ | ++ | ++ | + | + | ++ |
117 | +++ | +++ | + | + | +++ | +++ |
118 | ++ | ++ | - | ++ | ++ | +++ |
145 | ++ | ++ | ++ | - | ++ | ++ |
146 | + | + | + | ++ | ++ | ++ |
147 | ++ | + | + | + | +++ | +++ |
148 | + | + | + | + | ++ | ++ |
154 | ++ | +++ | ++ | + | +++ | ++ |
393 | + | + | + | + | ++ | + |
p-1-3-2 | ++ | - | - | + | ++ | ++ |
p-1-3-9 | +++ | ++ | ++ | ++ | ++++ | ++ |
p-1-7-4 | +++ | ++ | ++ | ++ | ++++ | ++ |
p-3-3-6 | ++ | ++ | + | ++ | +++ | ++ |
p-11-7-6 | - | - | - | - | - | - |
p-12-3-2 | ++++ | +++ | ++++ | ++++ | ++++ | ++++ |
p-13-7-11 | ++ | +++ | ++ | ++ | +++ | ++ |
p-14-3-2 | + | ++ | ++ | ++ | +++ | +++ |
p-14-7-3 | - | + | + | ++ | ++ | +++ |
M-4-3-1 | ++++ | ++++ | ++++ | ++++ | +++ | ++++ |
M-14-3-1 | ++ | ++ | ++ | + | + | ++ |
M17-6-3-2 | +++ | +++ | ++ | + | ++ | ++ |
S-1-3-1 | ++ | +++ | ++ | + | +++ | ++ |
S-3-3-1 | + | ++ | +++ | - | - | ++ |
S-5-3-2 | ++ | ++ | ++ | + | ++ | ++ |
S-11-3-1 | ++ | + | - | ++ | ++ | ++ |
S-13-7-2 | ++ | - | ++ | ++ | ++ | ++ |
S-14-7-5 | ++ | ++ | ++ | + | ++ | +++ |
L-4-3-2 | ++ | + | - | ++ | ++ | +++ |
L-11-7-2 | ++ | - | + | ++ | ++ | +++ |
L-13-7-7 | ++ | + | + | ++ | ++ | +++ |
L-14-7-3 | ++ | + | + | ++ | ++ | +++ |
Y-1 | ++++ | ++++ | +++ | ++++ | ++++ | ++++ |
Y-2 | ++ | ++ | - | ++ | ++ | +++ |
Y-3 | ++ | + | + | ++ | ++ | ++ |
Y-4 | + | + | + | + | ++ | ++++ |
Y-5 | +++ | ++ | ++ | +++ | ++++ | ++++ |
YT | ++++ | ++++ | ++++ | ++++ | ++++ | ++++ |
Grx10 | ++ | ++ | ++ | + | ++ | ++ |
Grx19 | ++ | + | + | ++ | +++ | +++ |
V9 | ++ | ++ | ++ | ++ | - | + |
LH100-1 | + | + | - | + | +++ | +++ |
LV108 | +++ | + | + | ++ | ++ | ++ |
R3 | +++ | - | + | + | + | + |
90-57 | ++ | + | + | ++ | +++ | +++ |
St KY | + | + | + | + | ++ | ++ |
S7 | ++ | + | - | + | ++ | - |
087-3 | + | + | + | ++ | ++ | ++ |
1.12.8 | ++ | + | - | ++ | ++ | ++ |
987-3 | - | - | - | - | - | - |
937-MQ | + | ++ | ++ | ++ | +++ | +++ |
975-1 | + | ++ | ++ | ++ | ++ | ++ |
XPL | + | + | + | ++ | ++ | ++ |
XPL-1-4 | - | - | - | - | - | - |
K01-4 | ++ | ++ | ++ | +++ | +++ | +++ |
JXR | +++ | - | + | + | + | +++ |
12-529 | + | + | - | + | ++ | ++ |
LD | +++ | ++ | + | ++ | ++ | +++ |
5-7-7-2 | ++ | ++ | + | + | ++ | ++ |
9-10-1-10 | + | - | + | ++ | ++ | + |
Strains | Initial pH of CFS | Inhibitory Zone of CFS against Indicator Strains (mm) | Inhibitory Zone of CFS with pH 5.0 against Indicator Strains (mm) | ||
---|---|---|---|---|---|
B. subtilis | S. enterica | B. subtilis | S. enterica | ||
Y-1 | 4.01 ± 0.01 a | 14.50 ± 0.41 c | 16.11 ± 0.08 e | 8.20 ± 0.41 b | 8.89 ± 0.57b c |
YT | 3.86 ± 0.03 c | 19.33 ± 0.47 a | 23.50 ± 0.41 a | 10.66 ± 0.29 a | 13.13 ± 0.19 a |
55 | 3.92 ± 0.02 b | 18.73 ± 0.21 a | 17.76 ± 0.20 d | 10.50 ± 0.41 a | 11.50 ± 0.41 b |
58 | 3.83 ± 0.03 c | 17.50 ± 0.08 b | 21.50 ± 0.41 b | 10.47 ± 0.37 a | 11.50 ± 0.40 b |
M-4-3-1 | 3.77 ± 0.01 d | 19.17 ± 0.24 a | 19.33 ± 0.24 c | 10.34 ± 0.29 a | 9.33 ± 0.24 c |
p-12-3-2 | 3.86 ± 0.03 c | 18.50 ± 0.41 a | 17.77 ± 0.21 d | 10.07 ± 0.09 a | 11.33 ± 0.27 b |
Strain | Inhibitory Zone (mm) | |
---|---|---|
B. subtilis | S. enterica | |
Y-1 | - | - |
YT | 12.00 ± 0.25 a | 12.50 ± 0.25 a |
55 | - | - |
58 | - | 10.12 ± 0.03 b |
M-4-3-1 | - | - |
p-12-3-2 | - | - |
pH | Diameter of Inhibitory Zone (mm) | |
---|---|---|
B. subtilis | S. enterica | |
3.0 | 18.56 ± 0.08 a | 22.50 ± 0.41 a |
4.0 | 10.10 ± 0.29 b | 12.49 ± 0.15 b |
5.0 | 3.72 ± 0.21 c | 5.87 ± 0.27 c |
6.0 | - | - |
7.0 | - | - |
8.0 | - | - |
9.0 | - | - |
Indicator Strains | Diameter of Inhibitory Zone (mm) |
---|---|
B. subtilis | 7.63 ± 0.85 b |
S. aureus | 8.00 ± 0.82 ab |
B. cereus | 8.13 ± 1.31 ab |
S. enterica | 9.13 ± 1.65 ab |
E. coli | 8.38 ± 0.95 ab |
P. brenneri | 9.88 ± 1.18 a |
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Guan, C.; Li, F.; Yu, P.; Chen, X.; Yin, Y.; Chen, D.; Gu, R.; Zhang, C.; Pang, B. Isolation, Identification and Antibacterial Characteristics of Lacticaseibacillus rhamnosus YT. Foods 2024, 13, 2706. https://doi.org/10.3390/foods13172706
Guan C, Li F, Yu P, Chen X, Yin Y, Chen D, Gu R, Zhang C, Pang B. Isolation, Identification and Antibacterial Characteristics of Lacticaseibacillus rhamnosus YT. Foods. 2024; 13(17):2706. https://doi.org/10.3390/foods13172706
Chicago/Turabian StyleGuan, Chengran, Feng Li, Peng Yu, Xuan Chen, Yongqi Yin, Dawei Chen, Ruixia Gu, Chenchen Zhang, and Bo Pang. 2024. "Isolation, Identification and Antibacterial Characteristics of Lacticaseibacillus rhamnosus YT" Foods 13, no. 17: 2706. https://doi.org/10.3390/foods13172706