Livestock-Associated Methicillin-Resistant Staphylococcus aureus (MRSA) in Purulent Subcutaneous Lesions of Farm Rabbits
Abstract
:1. Introduction
2. Materials and Methods
2.1. Samples and Bacterial Isolates
2.2. Antimicrobial Susceptibility Testing
2.3. Antibiotic Resistance Genes and Virulence Factors
2.4. Molecular Typing
3. Results
4. Discussion
4.1. Characterization of CC97 MRSA Isolates
4.2. Characterization of Non-CC97 MRSA Isolates
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
- Branco, M.; Monnerot, M.; Ferrand, N.; Templeton, A.R. Postglacial dispersal of the european rabbit (Oryctolagus cuniculus) on the iberian peninsula reconstructed from nested clade and mismatch analyses of mitochondrial dna genetic variation. Evolution (N. Y.) 2002, 56, 792–803. [Google Scholar]
- Brown, S.C.; Wells, K.; Roy-Dufresne, E.; Campbell, S.; Cooke, B.; Cox, T.; Fordham, D.A. Models of spatiotemporal variation in rabbit abundance reveal management hotspots for an invasive species. Ecol. Appl. 2020. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Rampin, F.; Piccirillo, A.; Schiavon, E.; Poppi, L.; Grilli, G. Detection of pathological lesions in slaughtered rabbits. Ital. J. Anim. Sci. 2008, 7, 105–111. [Google Scholar] [CrossRef]
- Hussain, M.A.; Dawson, C.O. Economic Impact of Food Safety Outbreaks on Food Businesses. Foods (Basel, Switzerland) 2013, 2, 585–589. [Google Scholar] [CrossRef] [Green Version]
- Jenni, L.; Janne, L. Compliance in slaughterhouses and control measures applied by official veterinarians. Food Control 2016, 68, 133–138. [Google Scholar] [CrossRef]
- Hermans, K.; Devriese, L.A.; Haesebrouck, F. Rabbit staphylococcosis: Difficult solutions for serious problems. Vet. Microbiol. 2003, 91, 57–64. [Google Scholar] [CrossRef]
- Rodríguez-Calleja, J.M.; García-López, I.; Santos, J.A.; Otero, A.; García-Lopez, M.-L. Molecular and phenotypic typing of Staphylococcus aureus isolates from rabbit meat. Res. Microbiol. 2006, 157, 496–502. [Google Scholar] [CrossRef]
- Gonzalez, C.D.; Ledo, C.; Cela, E.; Stella, I.; Xu, C.; Ojeda, D.S.; Frenette, P.S.; Gómez, M.I. The good side of inflammation: Staphylococcus aureus proteins SpA and Sbi contribute to proper abscess formation and wound healing during skin and soft tissue infections. Biochim. Biophys. Acta Mol. Basis Dis. 2019, 1865, 2657–2670. [Google Scholar] [CrossRef]
- Traverso, S.D.; da Cunha, L.; Fernandes, J.C.T.; Loretti, A.P.; Rhoden, A.; Wunder, E., Jr.; Driemeier, D. Mastite com lesões sistêmicas por Staphylococus aureus subesp. aureus em coelhos. Ciência Rural 2003, 33, 373–376. [Google Scholar] [CrossRef]
- Dalle Zotte, A. Perception of rabbit meat quality and major factors influencing the rabbit carcass and meat quality. Livest. Prod. Sci. 2002, 75, 11–32. [Google Scholar] [CrossRef]
- Haag, A.F.; Fitzgerald, J.R.; Penadés, J.R. Staphylococcus aureus in Animals. In Gram-Positive Pathogens; ASM Press: Dulles, VA, USA, 2019; pp. 731–746. [Google Scholar]
- Kadariya, J.; Smith, T.C.; Thapaliya, D. Staphylococcus aureus and Staphylococcal Food-Borne Disease: An Ongoing Challenge in Public Health. Biomed Res. Int. 2014, 2014, 827965. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Fisher, E.L.; Otto, M.; Cheung, G.Y.C. Basis of Virulence in Enterotoxin-Mediated Staphylococcal Food Poisoning. Front. Microbiol. 2018, 9, 436. [Google Scholar] [CrossRef] [PubMed]
- Yoon, H.J.; Choi, J.Y.; Lee, K.; Yong, D.; Kim, J.M.; Song, Y.G. Accessory gene regulator group polymorphisms in methicillin-resistant Staphylococcus aureus: An association with clinical significance. Yonsei Med. J. 2007, 48, 176–183. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Das, R.; Sebastian, S.; Kapil, A.; Dhawan, B. Decline of Nosocomial Methicillin-Resistant Staphylococcus aureus Skin and Soft Tissue Infections in an Indian Tertiary Hospital: Hope for the Future. J. Clin. Diagn. Res. 2017, 11, DL01–DL02. [Google Scholar] [CrossRef]
- Bengtsson-Palme, J.; Kristiansson, E.; Larsson, D.G.J. Environmental factors influencing the development and spread of antibiotic resistance. FEMS Microbiol. Rev. 2018, 42, fux053. [Google Scholar] [CrossRef]
- Feltrin, F.; Alba, P.; Kraushaar, B.; Ianzano, A.; Argudín, M.A.; Di Matteo, P.; Porrero, M.C.; Aarestrup, F.M.; Butaye, P.; Franco, A.; et al. A Livestock-Associated, Multidrug-Resistant, Methicillin-Resistant Staphylococcus aureus Clonal Complex 97 Lineage Spreading in Dairy Cattle and Pigs in Italy. Appl. Environ. Microbiol. 2016, 82, 816–821. [Google Scholar] [CrossRef] [Green Version]
- Elstrøm, P.; Grøntvedt, C.A.; Gabrielsen, C.; Stegger, M.; Angen, Ø.; Åmdal, S.; Enger, H.; Urdahl, A.M.; Jore, S.; Steinbakk, M.; et al. Livestock-Associated MRSA CC1 in Norway; Introduction to Pig Farms, Zoonotic Transmission, and Eradication. Front. Microbiol. 2019, 10, 139. [Google Scholar] [CrossRef] [Green Version]
- CLSI Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing; Clinical and Laboratory Standards Institute: Wayne, PA, USA, 2017. [Google Scholar]
- Silva, V.; Almeida, F.; Silva, A.; Correia, S.; Carvalho, J.A.; Castro, A.P.; Ferreira, E.; Manageiro, V.; Caniça, M.; Igrejas, G.; et al. First report of linezolid-resistant cfr-positive methicillin-resistant Staphylococcus aureus (MRSA) in humans in Portugal. J. Glob. Antimicrob. Resist. 2019, 17, 323–325. [Google Scholar] [CrossRef]
- Yu, F.; Liu, Y.; Lv, J.; Qi, X.; Lu, C.; Ding, Y.; Li, D.; Liu, H.; Wang, L. Antimicrobial susceptibility, virulence determinant carriage and molecular characteristics of Staphylococcus aureus isolates associated with skin and soft tissue infections. Braz. J. Infect. Dis. 2015, 19, 614–622. [Google Scholar] [CrossRef] [Green Version]
- Jarraud, S.; Mougel, C.; Thioulouse, J.; Lina, G.; Meugnier, H.; Forey, F.; Etienne, J.; Vandenesch, F.; Nesme, X. Relationships between Staphylococcus aureus Genetic Background, Virulence Factors, agr Groups (Alleles), and Human Disease. Infect. Immun. 2002, 70, 631–641. [Google Scholar] [CrossRef] [Green Version]
- Lina, G.; Piemont, Y.; Godail-Gamot, F.; Bes, M.; Peter, M.-O.; Gauduchon, V.; Vandenesch, F.; Etienne, J. Involvement of Panton-Valentine Leukocidin—Producing Staphylococcus aureus in Primary Skin Infections and Pneumonia. Clin. Infect. Dis. 1999, 29, 1128–1132. [Google Scholar] [CrossRef] [PubMed]
- van Wamel, W.J.B.; Rooijakkers, S.H.M.; Ruyken, M.; van Kessel, K.P.M.; van Strijp, J.A.G. The innate immune modulators staphylococcal complement inhibitor and chemotaxis inhibitory protein of Staphylococcus aureus are located on beta-hemolysin-converting bacteriophages. J. Bacteriol. 2006, 188, 1310–1315. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Shopsin, B.; Mathema, B.; Alcabes, P.; Said-Salim, B.; Lina, G.; Matsuka, A.; Martinez, J.; Kreiswirth, B.N. Prevalence of Specificity Groups among Staphylococcus aureus Strains Colonizing Children and Their Guardians. J. Clin. Microbiol. 2003, 41, 456–459. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Zhang, K.; McClure, J.-A.; Elsayed, S.; Louie, T.; Conly, J.M. Novel Multiplex PCR Assay for Characterization and Concomitant Subtyping of Staphylococcal Cassette Chromosome mec Types I to V in Methicillin-Resistant Staphylococcus aureus. J. Clin. Microbiol. 2005, 43, 5026–5033. [Google Scholar] [CrossRef] [Green Version]
- Enright, M.C.; Day, N.P.; Davies, C.E.; Peacock, S.J.; Spratt, B.G. Multilocus sequence typing for characterization of methicillin-resistant and methicillin-susceptible clones of Staphylococcus aureus. J. Clin. Microbiol. 2000, 38, 1008–1015. [Google Scholar] [CrossRef] [Green Version]
- Harmsen, D.; Claus, H.; Witte, W.; Rothgänger, J.; Claus, H.; Turnwald, D.; Vogel, U. Typing of Methicillin-Resistant Staphylococcus aureus in a University Hospital Setting by Using Novel Software for spa Repeat Determination and Database Management. J. Clin. Microbiol. 2003, 41, 5442–5448. [Google Scholar] [CrossRef] [Green Version]
- Corpa, J.M.; Hermans, K.; Haesebrouck, E. Main pathologies associated with Staphylococcus aureus infections in rabbits: A review. World Rabbit Sci. 2010, 17. [Google Scholar] [CrossRef] [Green Version]
- Agnoletti, F.; Mazzolini, E.; Bacchin, C.; Bano, L.; Berto, G.; Rigoli, R.; Muffato, G.; Coato, P.; Tonon, E.; Drigo, I. First reporting of methicillin-resistant Staphylococcus aureus (MRSA) ST398 in an industrial rabbit holding and in farm-related people. Vet. Microbiol. 2014, 170, 172–177. [Google Scholar] [CrossRef]
- Moreno-Grúa, E.; Pérez-Fuentes, S.; Muñoz-Silvestre, A.; Viana, D.; Fernández-Ros, A.B.; Sanz-Tejero, C.; Corpa, J.M.; Selva, L. Characterization of Livestock-Associated Methicillin-Resistant Staphylococcus aureus Isolates Obtained From Commercial Rabbitries Located in the Iberian Peninsula. Front. Microbiol. 2018, 9, 1812. [Google Scholar] [CrossRef] [Green Version]
- Silva, V.; Pereira, J.E.; Maltez, L.; Ferreira, E.; Manageiro, V.; Caniça, M.; Capelo, J.L.; Igrejas, G.; Poeta, P. Diversity of methicillin-resistant staphylococci among wild Lepus granatensis: First detection of mecA-MRSA in hares. FEMS Microbiol. Ecol. 2020, 96. [Google Scholar] [CrossRef]
- Cuny, C.; Wieler, L.; Witte, W. Livestock-Associated MRSA: The Impact on Humans. Antibiotics 2015, 4, 521–543. [Google Scholar] [CrossRef] [PubMed]
- Spoor, L.E.; McAdam, P.R.; Weinert, L.A.; Rambaut, A.; Hasman, H.; Aarestrup, F.M.; Kearns, A.M.; Larsen, A.R.; Skov, R.L.; Fitzgerald, J.R. Livestock Origin for a Human Pandemic Clone of Community-Associated Methicillin-Resistant Staphylococcus aureus. MBio 2013, 4, e00356-13. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Conceição, T.; de Lencastre, H.; Aires-de-Sousa, M. Healthy Bovines as Reservoirs of Major Pathogenic Lineages of Staphylococcus aureus in Portugal. Microb. Drug Resist. 2017, 23, 845–851. [Google Scholar] [CrossRef] [PubMed]
- Boswihi, S.S.; Udo, E.E.; Mathew, B.; Noronha, B.; Verghese, T.; Tappa, S.B. Livestock-Associated Methicillin-Resistant Staphylococcus aureus in Patients Admitted to Kuwait Hospitals in 2016–2017. Front. Microbiol. 2020, 10, 2912. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Merz, A.; Stephan, R.; Johler, S. Genotyping and DNA microarray based characterization of Staphylococcus aureus isolates from rabbit carcasses. Meat Sci. 2016, 112, 86–89. [Google Scholar] [CrossRef]
- van der Mee-Marquet, N.L.; Corvaglia, A.; Haenni, M.; Bertrand, X.; Franck, J.-B.; Kluytmans, J.; Girard, M.; Quentin, R.; François, P. Emergence of a novel subpopulation of CC398 Staphylococcus aureus infecting animals is a serious hazard for humans. Front. Microbiol. 2014, 5, 652. [Google Scholar] [CrossRef]
- Mitra, S.D.; Velu, D.; Bhuvana, M.; Krithiga, N.; Banerjee, A.; Shome, R.; Rahman, H.; Ghosh, S.K.; Shome, B.R. Staphylococcus aureus spa type t267, clonal ancestor of bovine subclinical mastitis in India. J. Appl. Microbiol. 2013, 114, 1604–1615. [Google Scholar] [CrossRef]
- Antri, K.; Akkou, M.; Bouchiat, C.; Bes, M.; Martins-Simoes, P.; Dauwalder, O.; Tristan, A.; Meugnier, H.; Rasigade, J.-P.; Etienne, J.; et al. High levels of Staphylococcus aureus and MRSA carriage in healthy population of Algiers revealed by additional enrichment and multisite screening. Eur. J. Clin. Microbiol. Infect. Dis. 2018, 37, 1521–1529. [Google Scholar] [CrossRef]
- Shore, A.C.; Lazaris, A.; Kinnevey, P.M.; Brennan, O.M.; Brennan, G.I.; Connell, B.; Feßler, A.T.; Schwarz, S.; Coleman, D.C. First Report of cfr-Carrying Plasmids in the Pandemic Sequence Type 22 Methicillin-Resistant Staphylococcus aureus Staphylococcal Cassette Chromosome mec Type IV Clone. Antimicrob. Agents Chemother. 2016, 60, 3007–3015. [Google Scholar] [CrossRef] [Green Version]
- Guo, D.; Liu, Y.; Han, C.; Chen, Z.; Ye, X. Phenotypic and molecular characteristics of methicillin-resistant and methicillin-susceptible Staphylococcus aureus isolated from pigs: Implication for livestock-association markers and vaccine strategies. Infect. Drug Resist. 2018, 11, 1299–1307. [Google Scholar] [CrossRef] [Green Version]
- Jaglic, Z.; Vlkova, H.; Bardon, J.; Michu, E.; Cervinkova, D.; Babak, V. Distribution, Characterization and Genetic Bases of Erythromycin Resistance in Staphylococci and Enterococci Originating from Livestock. Zoonoses Public Health 2012, 59, 202–211. [Google Scholar] [CrossRef] [PubMed]
- Roberts, M.C. Update on macrolide–lincosamide–streptogramin, ketolide, and oxazolidinone resistance genes. FEMS Microbiol. Lett. 2008, 282, 147–159. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- El-Ashker, M.; Gwida, M.; Monecke, S.; El-Gohary, F.; Ehricht, R.; Elsayed, M.; Akinduti, P.; El-Fateh, M.; Maurischat, S. Antimicrobial resistance pattern and virulence profile of S. aureus isolated from household cattle and buffalo with mastitis in Egypt. Vet. Microbiol. 2020, 240, 108535. [Google Scholar] [CrossRef] [PubMed]
- Khemiri, M.; Abbassi, M.S.; Couto, N.; Mansouri, R.; Hammami, S.; Pomba, C. Genetic characterisation of Staphylococcus aureus isolated from milk and nasal samples of healthy cows in Tunisia: First report of ST97-t267-agrI-SCCmec V MRSA of bovine origin in Tunisia. J. Glob. Antimicrob. Resist. 2018, 14, 161–165. [Google Scholar] [CrossRef]
- Tan, L.; Li, S.R.; Jiang, B.; Hu, X.M.; Li, S. Therapeutic Targeting of the Staphylococcus aureus Accessory Gene Regulator (agr) System. Front. Microbiol. 2018, 9, 55. [Google Scholar] [CrossRef]
- Chini, V.; Dimitracopoulos, G.; Spiliopoulou, I. Occurrence of the Enterotoxin Gene Cluster and the Toxic Shock Syndrome Toxin 1 Gene among Clinical Isolates of Methicillin-Resistant Staphylococcus aureus Is Related to Clonal Type Group. J. Clin. Microbiol. 2006, 44, 1881–1883. [Google Scholar] [CrossRef] [Green Version]
- Alba, P.; Feltrin, F.; Cordaro, G.; Porrero, M.C.; Kraushaar, B.; Argudín, M.A.; Nykäsenoja, S.; Monaco, M.; Stegger, M.; Aarestrup, F.M.; et al. Livestock-Associated Methicillin Resistant and Methicillin Susceptible Staphylococcus aureus Sequence Type (CC)1 in European Farmed Animals: High Genetic Relatedness of Isolates from Italian Cattle Herds and Humans. PLoS ONE 2015, 10, e0137143. [Google Scholar] [CrossRef] [Green Version]
- Becker, K.; Schaumburg, F.; Fegeler, C.; Friedrich, A.W.; Köck, R. Staphylococcus aureus from the German general population is highly diverse. Int. J. Med. Microbiol. 2017, 307, 21–27. [Google Scholar] [CrossRef]
- Santos, V.; Gomes, A.; Ruiz-Ripa, L.; Mama, O.M.; Sabença, C.; Sousa, M.; Silva, V.; Sousa, T.; Vieira-Pinto, M.; Igrejas, G.; et al. Methicillin-Resistant Staphylococcus aureus CC398 in Purulent Lesions of Piglets and Fattening Pigs in Portugal. Microb. Drug Resist. 2020. [Google Scholar] [CrossRef]
- Senok, A.C.; Somily, A.M.; Slickers, P.; Raji, M.A.; Garaween, G.; Shibl, A.; Monecke, S.; Ehricht, R. Investigating a rare methicillin-resistant Staphylococcus aureus strain: First description of genome sequencing and molecular characterization of CC15-MRSA. Infect. Drug Resist. 2017, 10, 307–315. [Google Scholar] [CrossRef] [Green Version]
- Silva, V.; Almeida, F.; Carvalho, J.A.; Castro, A.P.; Ferreira, E.; Manageiro, V.; Tejedor-Junco, M.T.; Caniça, M.; Igrejas, G.; Poeta, P. Emergence of community-acquired methicillin-resistant Staphylococcus aureus EMRSA-15 clone as the predominant cause of diabetic foot ulcer infections in Portugal. Eur. J. Clin. Microbiol. Infect. Dis. 2019, 39, 179–186. [Google Scholar] [CrossRef] [PubMed]
- Cuny, C.; Layer, F.; Hansen, S.; Werner, G.; Witte, W. Nasal Colonization of Humans with Occupational Exposure to Raw Meat and to Raw Meat Products with Methicillin-Susceptible and Methicillin-Resistant Staphylococcus aureus. Toxins (Basel) 2019, 11, 190. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Mama, O.M.; Morales, L.; Ruiz-Ripa, L.; Zarazaga, M.; Torres, C. High prevalence of multidrug resistant S. aureus-CC398 and frequent detection of enterotoxin genes among non-CC398 S. aureus from pig-derived food in Spain. Int. J. Food Microbiol. 2020, 320, 108510. [Google Scholar] [CrossRef] [PubMed]
- Wulf, M.; Voss, A. MRSA in livestock animals—An epidemic waiting to happen? Clin. Microbiol. Infect. 2008, 14, 519–521. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Petersen, A.; Stegger, M.; Heltberg, O.; Christensen, J.; Zeuthen, A.; Knudsen, L.K.; Urth, T.; Sorum, M.; Schouls, L.; Larsen, J.; et al. Epidemiology of methicillin-resistant Staphylococcus aureus carrying the novel mecC gene in Denmark corroborates a zoonotic reservoir with transmission to humans. Clin. Microbiol. Infect. 2013, 19, E16–E22. [Google Scholar] [CrossRef] [Green Version]
- Kadlec, K.; Feßler, A.T.; Hauschild, T.; Schwarz, S. Novel and uncommon antimicrobial resistance genes in livestock-associated methicillin-resistant Staphylococcus aureus. Clin. Microbiol. Infect. 2012, 18, 745–755. [Google Scholar] [CrossRef] [Green Version]
- Soares, G.M.S.; Figueiredo, L.C.; Faveri, M.; Cortelli, S.C.; Duarte, P.M.; Feres, M. Mechanisms of action of systemic antibiotics used in periodontal treatment and mechanisms of bacterial resistance to these drugs. J. Appl. Oral Sci. 2012, 20, 295–309. [Google Scholar] [CrossRef] [Green Version]
- Argudín, M.A.; Cariou, N.; Salandre, O.; Le Guennec, J.; Nemeghaire, S.; Butaye, P. Genotyping and antimicrobial resistance of Staphylococcus aureus isolates from diseased turkeys. Avian Pathol. 2013, 42, 572–580. [Google Scholar] [CrossRef] [Green Version]
- Sommer, M.O.A.; Church, G.M.; Dantas, G. The human microbiome harbors a diverse reservoir of antibiotic resistance genes. Virulence 2010, 1, 299–303. [Google Scholar] [CrossRef] [Green Version]
- Petinaki, E.; Spiliopoulou, I. Methicillin-resistant Staphylococcus aureus among companion and food-chain animals: Impact of human contacts. Clin. Microbiol. Infect. 2012, 18, 626–634. [Google Scholar] [CrossRef] [Green Version]
Isolates | Resistance | Virulence Factors | Molecular Characterization | ||||
---|---|---|---|---|---|---|---|
Phenotype | Genotype | IEC Type | Other Genes | ST/CC | spa-type | agr-type | |
VS2745 | FOX, PEN, TET, ERY, DA, CIP | mecA, tet(K), erm(C), vgaB | - | hlB, cna | ST2855/CC97 | t1190 | III |
VS2746 | FOX, PEN, TET, ERY, DA | mecA, tet(K), erm(C) | - | hlB | ST2855/CC97 | t1190 | III |
VS2747 | FOX, PEN, ERY, DA, CIP | mecA, erm(C) | - | hlB, cna | ST2855/CC97 | t1190 | III |
VS2748 | FOX, PEN, TET, ERY, DA, CN, TOB, CIP | mecA, tet(K), erm(B), erm(C), aac(6′)-Ie-aph(2″)-Ia | B | hlB, cna | ST105/CC5 | t002 | III |
VS2749 | FOX, PEN, TET, ERY, DA, CIP | mecA, tet(K), erm(C) | - | hlB, cna | ST2855/CC97 | t1190 | III |
VS2750 | FOX, PEN, TET, ERY, DA, CN, TOB, CIP | mecA, tet(K), erm(C), aac(6′)-Ie-aph(2″)-Ia | - | hlB, cna | ST2855/CC97 | t2802 | III |
VS2751 | FOX, PEN, TET, ERY, DA, CN, TOB, CIP | mecA, tet(K), erm(C), aac(6′)-Ie-aph(2″)-Ia | - | hlB | ST2855/CC97 | t2802 | III |
VS2752 | FOX, PEN, TET, ERY, DA, CN, TOB, CIP | mecA, tet(K), erm(C), aac(6′)-Ie-aph(2″)-Ia | - | hlB, cna | ST5/CC5 | t002 | III |
VS2753 | FOX, PEN, ERY, DA, TOB | mecA, erm(C), vgaA | - | hlB, cna | ST2855/CC97 | t2802 | III |
VS2754 | FOX, PEN, TET, ERY, DA, CN, CIP | mecA, tet(K), erm(C), aac(6′)-Ie-aph(2″)-Ia | - | hlB, cna | ST5/CC5 | t1094 | III |
VS2755 | FOX, PEN, ERY, DA, FD | mecA, erm(B), erm(C) | - | hlB, cna | ST5/CC5 | t002 | III |
VS2756 | FOX, PEN, ERY, DA, CIP, FD | mecA, erm(B), erm(C), msr(A/B) | C | hlB, etA, cna | ST582/CC15 | t084 | III |
VS2757 | FOX, PEN, ERY, DA, CIP, FD, | mecA, erm(C) | B | hlB, cna | ST5/CC5 | t002 | III |
VS2758 | FOX, PEN, ERY, DA, CN, TOB, CIP | mecA, erm(C), aac(6′)-Ie-aph(2″)-Ia | - | hlB | ST2855/CC97 | t2802 | III |
VS2759 | FOX, PEN, TET, ERY, DA, CIP, FD | mecA, tet(K), tet(L), erm(B), msr(A/B), linB | - | cna | ST22/CC22 | t032 | III |
VS2760 | FOX, PEN, TET, ERY, DA, CN, TOB, CIP | mecA, tet(K), erm(C), vgaB, aac(6′)-Ie-aph(2″)-Ia | - | hlB, cna | ST1/CC1 | t1491 | III |
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Silva, V.; de Sousa, T.; Gómez, P.; Sabença, C.; Vieira-Pinto, M.; Capita, R.; Alonso-Calleja, C.; Torres, C.; Capelo, J.L.; Igrejas, G.; et al. Livestock-Associated Methicillin-Resistant Staphylococcus aureus (MRSA) in Purulent Subcutaneous Lesions of Farm Rabbits. Foods 2020, 9, 439. https://doi.org/10.3390/foods9040439
Silva V, de Sousa T, Gómez P, Sabença C, Vieira-Pinto M, Capita R, Alonso-Calleja C, Torres C, Capelo JL, Igrejas G, et al. Livestock-Associated Methicillin-Resistant Staphylococcus aureus (MRSA) in Purulent Subcutaneous Lesions of Farm Rabbits. Foods. 2020; 9(4):439. https://doi.org/10.3390/foods9040439
Chicago/Turabian StyleSilva, Vanessa, Telma de Sousa, Paula Gómez, Carolina Sabença, Madalena Vieira-Pinto, Rosa Capita, Carlos Alonso-Calleja, Carmen Torres, José L. Capelo, Gilberto Igrejas, and et al. 2020. "Livestock-Associated Methicillin-Resistant Staphylococcus aureus (MRSA) in Purulent Subcutaneous Lesions of Farm Rabbits" Foods 9, no. 4: 439. https://doi.org/10.3390/foods9040439