Determination of Cyclopropenoid Fatty Acids in Ewe Milk Fat by GC-MS after Intravenous Administration of Sterculic Acid
Abstract
:1. Introduction
2. Materials and Methods
2.1. Reagents, Milk Samples, and Standards
2.2. Derivatization, Identification, and Quantification of Sterculic Acid by GC-MS
2.2.1. Sterculic Acid Standard Derivatization
2.2.2. Ewe Milk Fat Extraction and Derivatization
2.2.3. GC-MS Analysis
3. Results
3.1. GC-MS Determination of Sterculic Acid Chemically Synthesized Standard
3.2. GC-MS Analysis of FAMEs in Ewe Milk Fat
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Relative (%) 1 | ||||||||
---|---|---|---|---|---|---|---|---|
Compound | M+ (m/z) | RT (min) | pH = 5 | pH = 7 | ||||
Sterculene | 266 | 16.2 | 12.4 | ± | 17.0 | 3.6 | ± | 2.7 |
Sterculic acid | 366 | 18.0 | 78.4 | ± | 9.4 | 86.3 | ± | 4.5 |
Dihydrosterculic acid | 368 | 18.2 | 2.0 | ± | 1.3 | 3.7 | ± | 0.4 |
Sterculic acid isomer | 366 | 18.4 | 7.2 | ± | 6.3 | 6.3 | ± | 1.7 |
Timepoints | Sterculic Acid Concentration (mg/kg Total Fat) | |||||
---|---|---|---|---|---|---|
Ewe 1 | Ewe 2 | Ewe 3 | Ewe 4 | Ewe 5 | Ewe 6 | |
1st day-pre-treatment | <LOD 2 | <LOD | <LOD | <LOD | <LOD | <LOD |
5th day-pre-treatment | <LOD | <LOD | <LOD | <LOD | <LOD | <LOD |
4th day-treatment | 500 ± 40 | 760 ± 60 | 394 ± 1 | 500 ± 20 | 573 ± 23 | 654.1 ± 0.3 |
5th day-treatment | 620 ± 50 | 740 ± 70 | 555 ± 47 | 580 ± 40 | 620 ± 36 | 825.9 ± 0.1 |
1st day-post-treatment | 210 ± 33 | 281 ± 48 | 219 ± 4 | 296 ± 7 | 256 ± 5 | 309 ± 11 |
5th day-post-treatment | <LOD | <LOD | <LOD | <LOQ 3 | <LOQ | <LOQ |
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Lolli, V.; Toral, P.G.; Caligiani, A.; Gómez-Cortés, P. Determination of Cyclopropenoid Fatty Acids in Ewe Milk Fat by GC-MS after Intravenous Administration of Sterculic Acid. Foods 2020, 9, 901. https://doi.org/10.3390/foods9070901
Lolli V, Toral PG, Caligiani A, Gómez-Cortés P. Determination of Cyclopropenoid Fatty Acids in Ewe Milk Fat by GC-MS after Intravenous Administration of Sterculic Acid. Foods. 2020; 9(7):901. https://doi.org/10.3390/foods9070901
Chicago/Turabian StyleLolli, Veronica, Pablo G. Toral, Augusta Caligiani, and Pilar Gómez-Cortés. 2020. "Determination of Cyclopropenoid Fatty Acids in Ewe Milk Fat by GC-MS after Intravenous Administration of Sterculic Acid" Foods 9, no. 7: 901. https://doi.org/10.3390/foods9070901