Association of Urinary Glyphosate with All-Cause Mortality and Cardiovascular Mortality among Adults in NHANES 2013–2018: Role of Alkaline Phosphatase
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Design and Participants
2.2. Measurements of Glyphosate Exposure
2.3. Ascertainment of CVD Outcomes
2.4. Measurements of Serum ALP
2.5. Covariates
2.6. Statistical Analysis
3. Results
3.1. Baseline Characteristics of Selected Individuals
3.2. Urinary Glyphosate Exposure, All-Cause Mortality and CVD Mortality
3.3. Role of ALP in the Associations of Glyphosate with All-Cause Mortality and CVD Mortality
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Characteristics | Overall (N = 4031) | Females (N = 2048) | Males (N = 1983) | p |
---|---|---|---|---|
Age (years), Mean (SD) | 49.2 (17.5) | 48.9 (17.3) | 49.4 (17.6) | 0.360 |
PIR | 2.554 (1.634) | 2.516 (1.621) | 2.593 (1.646) | 0.154 |
Glyphosate (ng/mL), Mean (SD) | 0.498 (0.573) | 0.468 (0.542) | 0.529 (0.602) | 0.001 |
Creatinine (mg/dL), Mean (SD) | 123.48 (79.74) | 106.10 (72.00) | 141.43 (83.30) | <0.001 |
Race, frequency (percentage) | 0.238 | |||
Non-Hispanic Black | 806 (20.0%) | 420 (20.5%) | 386 (19.5%) | |
Non-Hispanic White | 1613 (40.0%) | 799 (39.0%) | 814 (41.0%) | |
Other Hispanic | 424 (10.5%) | 232 (11.3%) | 192 (9.7%) | |
Other Race | 1188 (29.5%) | 597 (29.1%) | 591 (29.8%) | |
Education, frequency (percentage) | 0.001 | |||
Less than high school | 800 (19.9%) | 367 (18.0%) | 433 (21.9%) | |
High school or equivalent | 901 (22.4%) | 440 (21.5%) | 461 (23.3%) | |
Some college or more | 2320 (57.8%) | 1236 (60.5%) | 1084 (54.8%) | |
BMI, frequency (percentage) | <0.001 | |||
<25 (kg/m2) | 1118 (27.9%) | 597 (29.3%) | 521 (26.5%) | |
≥25 (kg/m2)–<30 (kg/m2) | 1261 (31.5%) | 552 (27.1%) | 709 (36.0%) | |
≥30 (kg/m2) | 1627 (40.6%) | 890 (43.6%) | 737 (37.5%) | |
Alcohol exposure, frequency (percentage) | <0.001 | |||
Heavy | 418 (10.9%) | 114 (5.9%) | 304 (16.0%) | |
Moderate | 1607 (42.1%) | 762 (39.6%) | 845 (44.6%) | |
No | 1794 (47.0%) | 1048 (54.5%) | 746 (39.4%) | |
Nicotine exposure, frequency (percentage) | <0.001 | |||
≥LLOD | 2627 (67.0%) | 1254 (62.8%) | 1373 (71.4%) | |
<LLOD | 1292 (33.0%) | 743 (37.2%) | 549 (28.6%) | |
Physical activity, frequency (percentage) | <0.001 | |||
No | 2262 (56.1%) | 1279 (62.5%) | 983 (49.6%) | |
Moderate | 855 (21.2%) | 479 (23.4%) | 376 (19.0%) | |
Vigorous | 914 (22.7%) | 290 (14.2%) | 624 (31.5%) | |
Hypertension, frequency (percentage) | 0.099 | |||
Yes | 1723 (42.7%) | 849 (41.5%) | 874 (44.1%) | |
No | 2308 (57.3%) | 1199 (58.5%) | 1109 (55.9%) | |
Mortality, frequency (percentage) | 0.005 | |||
Assumed alive | 3807 (94.4%) | 1955 (95.5%) | 1852 (93.4%) | |
Assumed death | 224 (5.6%) | 93 (4.5%) | 131 (6.6%) | |
Cerebrovascular cause of death | 62 (1.5%) | 16 (0.8%) | 46 (2.3%) |
Weighted Cox Regression with Missing Values of Covariates Imputed | Unweighted Cox Regression with Missing Values of Covariates Imputed | Weighted Cox Regression with Missing Value Deleted | Unweighted Cox Regression with Missing Value Deleted | |||||
---|---|---|---|---|---|---|---|---|
HR (95%CI) | p | HR (95%CI) | p | HR (95%CI) | p | HR (95%CI) | p | |
All-cause mortality | ||||||||
Model 1 | 1.77 (1.46 to 2.16) | <0.001 | 1.67 (1.41 to 1.98) | <0.001 | 1.86 (1.54 to 2.24) | <0.001 | 1.71 (1.42 to 2.07) | <0.001 |
Model 2 | 1.61 (1.31 to 1.97) | <0.001 | 1.52 (1.28 to 1.80) | 0.012 | 1.67 (1.37 to 2.03) | <0.001 | 1.55 (1.28 to 1.88) | <0.001 |
Model 3 | 1.29 (1.05 to 1.59) | 0.017 | 1.15 (1.02 to 1.32) | 0.036 | 1.35 (1.08 to 1.69) | 0.008 | 1.17 (1.01 to 1.37) | 0.045 |
CVD mortality | ||||||||
Model 1 | 1.84 (1.42 to 2.38) | <0.001 | 1.67 (1.23 to 2.27) | 0.001 | 1.80 (1.38 to 2.35) | <0.001 | 1.70 (1.25 to 2.32) | 0.001 |
Model 2 | 1.67 (1.28 to 2.19) | <0.001 | 1.51 (1.11 to 2.06) | 0.009 | 1.60 (1.19 to 2.15) | 0.002 | 1.53 (1.12 to 2.09) | 0.007 |
Model 3 | 1.32 (1.02 to 1.70) | 0.042 | 1.12 (0.82 to 1.52) | 0.460 | 1.24 (0.87 to 1.78) | 0.234 | 1.11 (0.81 to 1.53) | 0.498 |
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Gao, Y.; Shu, S.; Zhang, D.; Wang, P.; Yu, X.; Wang, Y.; Yu, Y. Association of Urinary Glyphosate with All-Cause Mortality and Cardiovascular Mortality among Adults in NHANES 2013–2018: Role of Alkaline Phosphatase. Toxics 2024, 12, 559. https://doi.org/10.3390/toxics12080559
Gao Y, Shu S, Zhang D, Wang P, Yu X, Wang Y, Yu Y. Association of Urinary Glyphosate with All-Cause Mortality and Cardiovascular Mortality among Adults in NHANES 2013–2018: Role of Alkaline Phosphatase. Toxics. 2024; 12(8):559. https://doi.org/10.3390/toxics12080559
Chicago/Turabian StyleGao, Yongyue, Shuge Shu, Di Zhang, Pu Wang, Xiangyu Yu, Yucheng Wang, and Yongquan Yu. 2024. "Association of Urinary Glyphosate with All-Cause Mortality and Cardiovascular Mortality among Adults in NHANES 2013–2018: Role of Alkaline Phosphatase" Toxics 12, no. 8: 559. https://doi.org/10.3390/toxics12080559