A Nanostructured Protein Filtration Device for Possible Use in the Treatment of Alzheimer’s Disease—Concept and Feasibility after In Vivo Tests
Abstract
:1. Introduction
2. Materials and Methods
2.1. Development of the Nanoporous Ceramic Filter
2.2. Development of the CSF Filtration Device
2.3. Device Implantation on Mice
2.4. Nanoporous Ceramic Filter Assessment after a 4-Week Trial Period
3. Results
3.1. Development and Characterization of the Functionalized Nanoporous Ceramic Filter
3.2. Analysis of the Nanoporous Ceramic Filter after the 4-Week In Vivo Treatment
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Parameter | Units | Average Values |
---|---|---|
Ceramic filter thickness | µm | 48 |
Pore diameter | nm | 17 (±1.7) |
Pore period | nm | 42 (±4.2) |
Pore density | 1/cm2 | 3.2 × 1010 (±20%) |
Porosity | % | 12 |
Molecular Weight Cut-Off (MWCO) | kDa | 140 |
(Aβ) Diffusivity | % | 100 |
Element | Figure 12a | Figure 12b | Figure 12c | Figure 12d |
---|---|---|---|---|
C | 6.62 | 41.25 | 70.9 | 77.68 |
O | 36.82 | 23.13 | 9.25 | 7.23 |
Na | 0 | 0.04 | 0.42 | 0.46 |
Al | 51.43 | 31.89 | 14.49 | 10.22 |
Si | 0.17 | 0.08 | 0.46 | 0.42 |
P | 0.08 | 0.03 | 0.61 | 0.67 |
S | 3.7 | 2.61 | 1.85 | 1.28 |
Cl | 0 | 0.06 | 0.72 | 0.81 |
Ca | 0.04 | 0.02 | 0.55 | 0.36 |
Au | 0.96 | 0.88 | 0.77 | 0.79 |
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Schreiner, T.G.; Menéndez-González, M.; Adam, M.; Popescu, B.O.; Szilagyi, A.; Stanciu, G.D.; Tamba, B.I.; Ciobanu, R.C. A Nanostructured Protein Filtration Device for Possible Use in the Treatment of Alzheimer’s Disease—Concept and Feasibility after In Vivo Tests. Bioengineering 2023, 10, 1303. https://doi.org/10.3390/bioengineering10111303
Schreiner TG, Menéndez-González M, Adam M, Popescu BO, Szilagyi A, Stanciu GD, Tamba BI, Ciobanu RC. A Nanostructured Protein Filtration Device for Possible Use in the Treatment of Alzheimer’s Disease—Concept and Feasibility after In Vivo Tests. Bioengineering. 2023; 10(11):1303. https://doi.org/10.3390/bioengineering10111303
Chicago/Turabian StyleSchreiner, Thomas Gabriel, Manuel Menéndez-González, Maricel Adam, Bogdan Ovidiu Popescu, Andrei Szilagyi, Gabriela Dumitrita Stanciu, Bogdan Ionel Tamba, and Romeo Cristian Ciobanu. 2023. "A Nanostructured Protein Filtration Device for Possible Use in the Treatment of Alzheimer’s Disease—Concept and Feasibility after In Vivo Tests" Bioengineering 10, no. 11: 1303. https://doi.org/10.3390/bioengineering10111303