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Article

Improving Bioactive Characteristics of Small Diameter Polytetrafluoroethylene Stent Grafts by Electrospinning: A Comparative Hemocompatibility Study

1
Department of Thoracic and Cardiovascular Surgery, University Hospital Tuebingen, Calwerstraße 7/1, 72076 Tuebingen, Germany
2
Department of Diagnostic and Interventional Radiology, University Hospital Tuebingen, Hoppe-Seyler-Strasse 3, 72076 Tuebingen, Germany
3
LimFlow SA, 15 Rue Traversière, 75012 Paris, France
4
Department of Surgery, Northwest Clinics, 1815 JD Alkmaar, The Netherlands
*
Author to whom correspondence should be addressed.
Bioengineering 2023, 10(4), 411; https://doi.org/10.3390/bioengineering10040411
Submission received: 12 February 2023 / Revised: 11 March 2023 / Accepted: 21 March 2023 / Published: 26 March 2023
(This article belongs to the Special Issue Engineering Biodegradable-Implant Materials, 2nd Edition)

Abstract

Polytetrafluoroethylene (PTFE) is a commonly used biomaterial for the manufacturing of vascular grafts and several strategies, such as coatings, have been explored to improve the hemocompatibility of small-diameter prostheses. In this study, the hemocompatibility properties of novel stent grafts covered with electrospun PTFE (LimFlow Gen-1 and LimFlow Gen-2) were compared with uncoated and heparin-coated PTFE grafts (Gore Viabahn®) using fresh human blood in a Chandler closed-loop system. After 60 min of incubation, the blood samples were examined hematologically and activation of coagulation, platelets, and the complement system were analyzed. In addition, the adsorbed fibrinogen on the stent grafts was measured and the thrombogenicity was assessed by SEM. Significantly lower adsorption of fibrinogen was measured on the surface of heparin-coated Viabahn than on the surface of the uncoated Viabahn. Furthermore, LimFlow Gen-1 stent grafts showed lower fibrinogen adsorption than the uncoated Viabahn®, and the LimFlow Gen-2 stent grafts showed comparable fibrinogen adsorption as the heparin-coated Viabahn®. SEM analysis revealed no sign of thrombus formation on any of the stent surfaces. LimFlow Gen-2 stent grafts covered with electrospun PTFE exhibited bioactive characteristics and revealed improved hemocompatibility in terms of reduced adhesion of fibrinogen, activation of platelets, and coagulation (assessed by β-TG and TAT levels) similar to heparin-coated ePTFE prostheses. Thus, this study demonstrated improved hemocompatibility of electrospun PTFE. The next step is to conduct in vivo studies to confirm whether electrospinning-induced changes to the PTFE surface can reduce the risk of thrombus formation and provide clinical benefits.
Keywords: polytetrafluoroethylene stent graft; electrospinning; hemocompatibility polytetrafluoroethylene stent graft; electrospinning; hemocompatibility

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MDPI and ACS Style

Avci-Adali, M.; Grözinger, G.; Cabane, V.; Schreve, M.; Wendel, H.P. Improving Bioactive Characteristics of Small Diameter Polytetrafluoroethylene Stent Grafts by Electrospinning: A Comparative Hemocompatibility Study. Bioengineering 2023, 10, 411. https://doi.org/10.3390/bioengineering10040411

AMA Style

Avci-Adali M, Grözinger G, Cabane V, Schreve M, Wendel HP. Improving Bioactive Characteristics of Small Diameter Polytetrafluoroethylene Stent Grafts by Electrospinning: A Comparative Hemocompatibility Study. Bioengineering. 2023; 10(4):411. https://doi.org/10.3390/bioengineering10040411

Chicago/Turabian Style

Avci-Adali, Meltem, Gerd Grözinger, Vincent Cabane, Michiel Schreve, and Hans Peter Wendel. 2023. "Improving Bioactive Characteristics of Small Diameter Polytetrafluoroethylene Stent Grafts by Electrospinning: A Comparative Hemocompatibility Study" Bioengineering 10, no. 4: 411. https://doi.org/10.3390/bioengineering10040411

APA Style

Avci-Adali, M., Grözinger, G., Cabane, V., Schreve, M., & Wendel, H. P. (2023). Improving Bioactive Characteristics of Small Diameter Polytetrafluoroethylene Stent Grafts by Electrospinning: A Comparative Hemocompatibility Study. Bioengineering, 10(4), 411. https://doi.org/10.3390/bioengineering10040411

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