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Open AccessArticle
In Vitro Bioassay for Damage-Associated Molecular Patterns Arising from Injured Oral Cells
by
Layla Panahipour
Layla Panahipour 1
,
Chiara Micucci
Chiara Micucci 1,
Benedetta Gelmetti
Benedetta Gelmetti 1 and
Reinhard Gruber
Reinhard Gruber 1,2,3,*
1
Department of Oral Biology, University Clinic of Dentistry, Medical University of Vienna, 1090 Vienna, Austria
2
Department of Periodontology, School of Dental Medicine, University of Bern, 3010 Bern, Switzerland
3
Austrian Cluster for Tissue Regeneration, 1200 Vienna, Austria
*
Author to whom correspondence should be addressed.
Bioengineering 2024, 11(7), 687; https://doi.org/10.3390/bioengineering11070687 (registering DOI)
Submission received: 29 May 2024
/
Revised: 27 June 2024
/
Accepted: 1 July 2024
/
Published: 5 July 2024
Abstract
Gingival fibroblasts are a significant source of paracrine signals required to maintain periodontal homeostasis and to mediate pathological events linked to periodontitis and oral squamous cell carcinomas. Among the potential paracrine signals are stanniocalcin-1 (STC1), involved in oxidative stress and cellular survival; amphiregulin (AREG), a growth factor that mediates the cross-talk between immune cells and epithelial cells; chromosome 11 open reading frame 96 (C11orf96) with an unclear biologic function; and the inflammation-associated prostaglandin E synthase (PTGES). Gingival fibroblasts increasingly express these genes in response to bone allografts containing remnants of injured cells. Thus, the gene expression might be caused by the local release of damage-associated molecular patterns arising from injured cells. The aim of this study is consequently to use the established gene panel as a bioassay to measure the damage-associated activity of oral cell lysates. To this aim, we have exposed gingival fibroblasts to lysates prepared from the squamous carcinoma cell lines TR146 and HSC2, oral epithelial cells, and gingival fibroblasts. We report here that all lysates significantly increased the transcription of the entire gene panel, supported for STC1 at the protein level. Blocking TGF-β receptor 1 kinase with SB431542 only partially reduced the forced expression of STC1, AREG, and C11orf96. SB431542 even increased the PTGES expression. Together, these findings suggest that the damage signals originating from oral cells can change the paracrine activity of gingival fibroblasts. Moreover, the expression panel of genes can serve as a bioassay for testing the biocompatibility of materials for oral application.
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MDPI and ACS Style
Panahipour, L.; Micucci, C.; Gelmetti, B.; Gruber, R.
In Vitro Bioassay for Damage-Associated Molecular Patterns Arising from Injured Oral Cells. Bioengineering 2024, 11, 687.
https://doi.org/10.3390/bioengineering11070687
AMA Style
Panahipour L, Micucci C, Gelmetti B, Gruber R.
In Vitro Bioassay for Damage-Associated Molecular Patterns Arising from Injured Oral Cells. Bioengineering. 2024; 11(7):687.
https://doi.org/10.3390/bioengineering11070687
Chicago/Turabian Style
Panahipour, Layla, Chiara Micucci, Benedetta Gelmetti, and Reinhard Gruber.
2024. "In Vitro Bioassay for Damage-Associated Molecular Patterns Arising from Injured Oral Cells" Bioengineering 11, no. 7: 687.
https://doi.org/10.3390/bioengineering11070687
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