This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Open AccessCommunication
A Bioreactor for Celullarised Membrane Culture and Delivery under Sterile Conditions
by
Ainitze Gereka
Ainitze Gereka 1,
Uzuri Urtaza
Uzuri Urtaza 1,
Pablo Larreategi
Pablo Larreategi 1,
Felipe Prosper
Felipe Prosper 2,
Enrique José Andreu
Enrique José Andreu 2,* and
Ane Miren Zaldua
Ane Miren Zaldua 1,*
1
Health Specialization, Leartiker S. Coop., 48270 Markina-Xemein, Spain
2
Cell Therapy Area, Clinica Universidad de Navarra, 31008 Pamplona, Spain
*
Authors to whom correspondence should be addressed.
Bioengineering 2024, 11(8), 785; https://doi.org/10.3390/bioengineering11080785 (registering DOI)
Submission received: 25 June 2024
/
Revised: 24 July 2024
/
Accepted: 24 July 2024
/
Published: 2 August 2024
Abstract
A novel, user-friendly bioreactor for the cultivation of cellularised membranes for tissue engineering has been successfully designed, manufactured, and validated. This bioreactor features a culture vessel and a cover, the latter equipped with one or more sidewalls to ensure airtightness in two distinct zones, thereby maintaining sterile conditions. The cover, designed to integrate seamlessly with the culture vessel, includes several ports compatible with commercial connectors. This design allows the introduction of cells and culture medium without requiring the opening of the cover, thus preserving sterility. Additionally, the cover is equipped with flanges that effectively press the membrane against the bottom surface of the culture vessel, preventing it from shrinking or shifting. This ensures that cells can properly adhere to the membrane and proliferate. Manufactured under Good Manufacturing Practice (GMP) conditions, the bioreactor supports cultivation in optimal aseptic environments, thereby preventing external contamination. This feature is critical for the safe transportation of cultivated tissue to clinical settings. Validation tests have confirmed the bioreactor’s excellent performance, endorsing its suitability for intended applications in tissue engineering.
Share and Cite
MDPI and ACS Style
Gereka, A.; Urtaza, U.; Larreategi, P.; Prosper, F.; Andreu, E.J.; Zaldua, A.M.
A Bioreactor for Celullarised Membrane Culture and Delivery under Sterile Conditions. Bioengineering 2024, 11, 785.
https://doi.org/10.3390/bioengineering11080785
AMA Style
Gereka A, Urtaza U, Larreategi P, Prosper F, Andreu EJ, Zaldua AM.
A Bioreactor for Celullarised Membrane Culture and Delivery under Sterile Conditions. Bioengineering. 2024; 11(8):785.
https://doi.org/10.3390/bioengineering11080785
Chicago/Turabian Style
Gereka, Ainitze, Uzuri Urtaza, Pablo Larreategi, Felipe Prosper, Enrique José Andreu, and Ane Miren Zaldua.
2024. "A Bioreactor for Celullarised Membrane Culture and Delivery under Sterile Conditions" Bioengineering 11, no. 8: 785.
https://doi.org/10.3390/bioengineering11080785
Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details
here.
Article Metrics
Article metric data becomes available approximately 24 hours after publication online.