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Open AccessArticle
Perlecan: An Islet Basement Membrane Protein with Protective Anti-Inflammatory Characteristics
by
Daniel Brandhorst
Daniel Brandhorst 1,2,*,†,
Heide Brandhorst
Heide Brandhorst 1,2,†,
Samuel Acreman
Samuel Acreman 1
and
Paul R. V. Johnson
Paul R. V. Johnson 1,2
1
Islet Transplant Research Group, Nuffield Department of Surgical Sciences, University of Oxford, Oxford OX3 9DU, UK
2
Oxford Consortium for Islet Transplantation, Oxford Centre for Diabetes, Endocrinology, and Metabolism (OCDEM), Churchill Hospital, University of Oxford, Oxford OX3 7LE, UK
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this study.
Bioengineering 2024, 11(8), 828; https://doi.org/10.3390/bioengineering11080828 (registering DOI)
Submission received: 28 May 2024
/
Revised: 7 August 2024
/
Accepted: 10 August 2024
/
Published: 13 August 2024
Abstract
Throughout the isolation process, human islets are subjected to destruction of the islet basement membrane (BM) and reduced oxygen supply. Reconstruction of the BM represents an option to improve islet function and survival post-transplant and may particularly be relevant for islet encapsulation devices and scaffolds. In the present study, we assessed whether Perlecan, used alone or combined with the BM proteins (BMPs) Collagen-IV and Laminin-521, has the ability to protect isolated human islets from hypoxia-induced damage. Islets isolated from the pancreas of seven different organ donors were cultured for 4–5 days at 2% oxygen in plain CMRL (sham-treated controls) or in CMRL supplemented with BMPs used either alone or in combination. Postculture, islets were characterized regarding survival, in vitro function and production of chemokines and reactive oxygen species (ROS). Individually added BMPs significantly doubled islet survival and increased in vitro function. Combining BMPs did not provide a synergistic effect. Among the tested BMPs, Perlecan demonstrated the significantly strongest inhibitory effect on chemokine and ROS production when compared with sham-treatment (p < 0.001). Perlecan may be useful to improve islet survival prior to and after transplantation. Its anti-inflammatory potency should be considered to optimise encapsulation and scaffolds to protect isolated human islets post-transplant.
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MDPI and ACS Style
Brandhorst, D.; Brandhorst, H.; Acreman, S.; Johnson, P.R.V.
Perlecan: An Islet Basement Membrane Protein with Protective Anti-Inflammatory Characteristics. Bioengineering 2024, 11, 828.
https://doi.org/10.3390/bioengineering11080828
AMA Style
Brandhorst D, Brandhorst H, Acreman S, Johnson PRV.
Perlecan: An Islet Basement Membrane Protein with Protective Anti-Inflammatory Characteristics. Bioengineering. 2024; 11(8):828.
https://doi.org/10.3390/bioengineering11080828
Chicago/Turabian Style
Brandhorst, Daniel, Heide Brandhorst, Samuel Acreman, and Paul R. V. Johnson.
2024. "Perlecan: An Islet Basement Membrane Protein with Protective Anti-Inflammatory Characteristics" Bioengineering 11, no. 8: 828.
https://doi.org/10.3390/bioengineering11080828
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