Platelet-Rich Plasma Lysate-Incorporating Gelatin Hydrogel as a Scaffold for Bone Reconstruction
Abstract
:1. Introduction
2. Materials and Methods
2.1. Rat MSC Expansion and Differentiation Assay
2.2. PRP Lysate Preparation
2.3. PRP Standardization and Growth Factors Quantification
2.4. Gtn–HPA Gel Formation
3. Experimental Protocols
3.1. Core-Ring 3D Assembly Migration Assay (n = 6) [32]
3.2. Assessing Cell Osteogenic Differentiation in PRP-Lysate-Infused Cell-Seeded Hydrogel
3.3. The Release Profile of PDGF-BB from Gels Incorporating PRP Lysate and Recombinant PDGF-BB
- (1)
- 2% Gtn–HPA blank hydrogel;
- (2)
- PRP lysate preparation I incorporated into Gtn–HPA;
- (3)
- PRP lysate preparation II incorporated into Gtn–HPA;
- (4)
- Recombinant rat PDGF-BB (50 ng/mL) incorporated into Gtn–HPA.
4. Results
4.1. Cell Migration into the Gtn–HPA Hydrogel
4.2. Osteogenic Differentiation of MSCs in the Gtn–HPA Hydrogel
4.3. ELISA Release Assay
5. Discussion
6. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Contribution to the Field Statement
References
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Nadra, M.; Niu, W.; Kurisawa, M.; Rousson, D.; Spector, M. Platelet-Rich Plasma Lysate-Incorporating Gelatin Hydrogel as a Scaffold for Bone Reconstruction. Bioengineering 2022, 9, 513. https://doi.org/10.3390/bioengineering9100513
Nadra M, Niu W, Kurisawa M, Rousson D, Spector M. Platelet-Rich Plasma Lysate-Incorporating Gelatin Hydrogel as a Scaffold for Bone Reconstruction. Bioengineering. 2022; 9(10):513. https://doi.org/10.3390/bioengineering9100513
Chicago/Turabian StyleNadra, Meral, Wanting Niu, Motoichi Kurisawa, Dominique Rousson, and Myron Spector. 2022. "Platelet-Rich Plasma Lysate-Incorporating Gelatin Hydrogel as a Scaffold for Bone Reconstruction" Bioengineering 9, no. 10: 513. https://doi.org/10.3390/bioengineering9100513