Optimization and Validation of Analytical Methodology for Determination of Pesticides in Grape, Must and Wine Samples with QuEChERS Extraction and Gas Chromatography–Mass Spectrometry

Sykalia, Dionysia L.; Trantopoulos, Epameinondas P.; Tsoutsi, Charoula S.; Albanis, Triantafyllos A.

doi:10.3390/beverages10030053

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Optimization and Validation of Analytical Methodology for Determination of Pesticides in Grape, Must and Wine Samples with QuEChERS Extraction and Gas Chromatography–Mass Spectrometry

by

Dionysia L. Sykalia

¹,

Epameinondas P. Trantopoulos

¹,

Charoula S. Tsoutsi

^1,2,*

and

Triantafyllos A. Albanis

^1,2

¹

Department of Chemistry, University of Ioannina, 45110 Ioannina, Greece

²

Institute of Environment and Sustainable Development, University Research Center of Ioannina (URCI), 45110 Ioannina, Greece

^*

Author to whom correspondence should be addressed.

Beverages 2024, 10(3), 53; https://doi.org/10.3390/beverages10030053

Submission received: 16 May 2024 / Revised: 25 June 2024 / Accepted: 27 June 2024 / Published: 3 July 2024

(This article belongs to the Section Wine, Spirits and Oenological Products)

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Abstract

This study is focused on the optimization and application of an analytical methodology for the determination of 15 selected pesticides in three substrates during the vinification process. QuEChERS extraction was used followed by GC-MS to develop a simple and rapid method for the determination of these compounds. The optimized methodology was validated, providing for all selected pesticides excellent linearity, recoveries ranging between 60.9% and 95.0% and LOQs lower than 50 ng g⁻¹ f for all substrates. The developed methodology was tested on real samples of grapes, must and wine obtained from a winery in the Epirus region. The results indicated the existence of some of the selected pesticides at comparatively low levels in contrast to the valid MRLs.

Keywords: QuEChERS; gas chromatography–mass spectrometry; pesticides; vinification process; analytical methodology

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MDPI and ACS Style

Sykalia, D.L.; Trantopoulos, E.P.; Tsoutsi, C.S.; Albanis, T.A. Optimization and Validation of Analytical Methodology for Determination of Pesticides in Grape, Must and Wine Samples with QuEChERS Extraction and Gas Chromatography–Mass Spectrometry. Beverages 2024, 10, 53. https://doi.org/10.3390/beverages10030053

AMA Style

Sykalia DL, Trantopoulos EP, Tsoutsi CS, Albanis TA. Optimization and Validation of Analytical Methodology for Determination of Pesticides in Grape, Must and Wine Samples with QuEChERS Extraction and Gas Chromatography–Mass Spectrometry. Beverages. 2024; 10(3):53. https://doi.org/10.3390/beverages10030053

Chicago/Turabian Style

Sykalia, Dionysia L., Epameinondas P. Trantopoulos, Charoula S. Tsoutsi, and Triantafyllos A. Albanis. 2024. "Optimization and Validation of Analytical Methodology for Determination of Pesticides in Grape, Must and Wine Samples with QuEChERS Extraction and Gas Chromatography–Mass Spectrometry" Beverages 10, no. 3: 53. https://doi.org/10.3390/beverages10030053

APA Style

Sykalia, D. L., Trantopoulos, E. P., Tsoutsi, C. S., & Albanis, T. A. (2024). Optimization and Validation of Analytical Methodology for Determination of Pesticides in Grape, Must and Wine Samples with QuEChERS Extraction and Gas Chromatography–Mass Spectrometry. Beverages, 10(3), 53. https://doi.org/10.3390/beverages10030053

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Optimization and Validation of Analytical Methodology for Determination of Pesticides in Grape, Must and Wine Samples with QuEChERS Extraction and Gas Chromatography–Mass Spectrometry

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